1887

Abstract

Bat species represent natural reservoirs for a number of high-consequence human pathogens. The present study investigated the diversity of polyomaviruses (PyVs) in Zambian insectivorous and fruit bat species. We describe the complete genomes from four newly proposed African bat PyV species employing the recently recommended criteria provided by the Study Group of the International Committee on Taxonomy of Viruses. A comprehensive phylogenetic and recombination analysis was performed to determine genetic relationships and the distribution of recombination events in PyV from mammalian and avian species. The novel species of PyV from Zambian bats segregated with members of the genera and , forming monophyletic clades with bat and non-human primate PyVs. and segregated in a clade with South American bat PyV species, Old World monkey and chimpanzee PyVs and (New Jersey PyV). Interestingly, the newly described Egyptian fruit bat PyV, tentatively named , had the highest nucleotide sequence identity to species of PyV from Indonesian fruit bats, and was most closely related to New World monkey PyVs. The distribution of recombination events in PyV genomes was non-random: recombination boundaries existed in the intergene region between VP1 and LTAg and also at the 3′ end of VP2/3 in the structural genes, whereas infrequent recombination was present within the LTAg gene. These findings indicate that recombination within the LTAg gene has been negatively selected against during polyomaviral evolution and support the recent proposal for taxonomic classification based on LTAg to define novel PyV species.

Keyword(s): bat , hot spot , LTAg , polyomavirus , recombination and Zambia
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2017-04-01
2024-12-13
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