1887

Abstract

Viral covert mortality disease (VCMD) has caused serious losses to shrimp aquaculture in China in recent years and the ridgetail white prawn Exopalaemon carinicauda has been suspected to be one important factor in perpetuating the high prevalence of covert mortality nodavirus (CMNV) infections due to its perennial presence in shrimp farming ponds and water from natural habitats. Experiments were carried out to determine the possibility of vertical transmission of CMNV in E. carinicauda in this study. CMNV infection in gonads, fertilized eggs and larvae was investigated by using the methods of reverse transcription nested PCR (nRT-PCR), in situ hybrization (ISH) and transmission electron microscopy (TEM). The ovarian tissue and testis tissue of artificially infected parental E. carinicauda were proved to be CMNV-positive by nRT-PCR. Fertilized eggs were also found to be CMNV-positive by nRT-PCR whether the fertilized eggs originated from the CMNV-positive female broodstock mated with the CMNV-negative male broodstock, or they originated from the CMNV-negative female broodstock mated with the CMNV-positive males. The results of ISH indicated that the positive signals were evident in the oocytes within ovarian tissue and nauplii. By TEM analysis, CMNV virions were observed in oogonia, oocytes, spermatocytes, fertilized eggs and nauplii. The presence of CMNV in fertilized eggs and larvae indicates that CMNV can transmit vertically via sperm and oocytes in E. carinicauda, which highlights the high probability of vertical transmission of CMNV in the main species of cultured shrimp and prawns.

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2017-04-28
2019-10-18
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References

  1. Zhang QH. To be cautious of “bottom death” in the intensive farming of Pacific white shrimp. Sci Fish Farming 2004; 10: 48– 49
    [Google Scholar]
  2. Xing H. Discussion of the control measures for the “bottom death” (covert mortality disease) of Pacific white shrimp. China Fish 2004; 4: 88– 89
    [Google Scholar]
  3. Song SX, Zhuang SP. Measures for control of “bottom death” of Pacific white shrimp. Fish Sci Technol 2006; 6: 36– 39
    [Google Scholar]
  4. Xu ZJ, Ji F. Comprehensive control of the covert mortality disease of Pacific white shrimp. Fish Guide Rich 2009; 1: 60 (in Chinese)
    [Google Scholar]
  5. Gu SJ. Analysis of causes of the covert mortality disease of Pacific white shrimp and its control strategies. Sci Fish Farming 2012; 8: 62– 63
    [Google Scholar]
  6. Zhang Q, Liu Q, Liu S, Yang H, Liu S et al. A new nodavirus is associated with covert mortality disease of shrimp. J Gen Virol 2014; 95: 2700– 2709 [CrossRef] [PubMed]
    [Google Scholar]
  7. Thitamadee S, Prachumwat A, Srisala J, Jaroenlak P, Salachan PV et al. Review of current disease threats for cultivated penaeid shrimp in Asia. Aquaculture 2016; 452: 69– 87 [Crossref]
    [Google Scholar]
  8. Pooljun C, Direkbusarakom S, Chotipuntu P, Hirono I, Wuthisuthimethavee S. Development of a TaqMan real-time RT-PCR assay for detection of covert mortality nodavirus (CMNV) in penaeid shrimp. Aquaculture 2016; 464: 445– 450 [Crossref]
    [Google Scholar]
  9. Qian D, Shi Z, Zhang S, Cao Z, Liu W et al. Extra small virus-like particles (XSV) and nodavirus associated with whitish muscle disease in the giant freshwater prawn, Macrobrachium rosenbergii. J Fish Dis 2003; 26: 521– 527 [PubMed] [Crossref]
    [Google Scholar]
  10. Bonami JR, Shi Z, Qian D, Sri WJ. White tail disease of the giant freshwater prawn, Macrobrachium rosenbergii: separation of the associated virions and characterization of MrNV as a new type of nodavirus. J Fish Dis 2005; 28: 23– 31 [CrossRef] [PubMed]
    [Google Scholar]
  11. Senapin S, Phiwsaiya K, Gangnonngiw W, Briggs M, Sithigorngul P et al. Dual infections of IMNV and MrNV in cultivated Penaeus vannamei from Indonesia. Aquaculture 2013; 372–375: 70– 73 [Crossref]
    [Google Scholar]
  12. Naveenkumar S, Shekar M, Karunasagar I, Karunasagar I. Genetic analysis of RNA1 and RNA2 of Macrobrachium rosenbergii nodavirus (MrNV) isolated from India. Virus Res 2013; 173: 377– 385 [CrossRef] [PubMed]
    [Google Scholar]
  13. Sudhakaran R, Syed Musthaq S, Haribabu P, Mukherjee SC, Gopal C et al. Experimental transmission of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) in three species of marine shrimp (Penaeus indicus, Penaeus japonicus and Penaeus monodon). Aquaculture 2006; 257: 136– 141 [Crossref]
    [Google Scholar]
  14. Sudhakaran R, Haribabu P, Kumar SR, Sarathi M, Ahmed VP et al. Natural aquatic insect carriers of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV). Dis Aquat Org 2008; 79: 141– 145 [CrossRef] [PubMed]
    [Google Scholar]
  15. Wang XQ, Yan BL, Ma S, Dong SL. Study on the biology and cultural ecology of Exopalaemon carinicauda. Shandong Fisheries 2005; 22: 21– 23
    [Google Scholar]
  16. Wj X, Xie JJ, Shi H, Cw L. Hematodinium infections in cultured ridgetail white prawns, Exopalaemon carinicauda, in eastern China. Aquaculture 2010; 300: 25– 31 [Crossref]
    [Google Scholar]
  17. Sudhakaran R, Ishaq Ahmed VP, Haribabu P, Mukherjee SC, Sri Widada J et al. Experimental vertical transmission of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) from brooders to progeny in Macrobrachium rosenbergii and Artemia. J Fish Dis 2007; 30: 27– 35 [CrossRef] [PubMed]
    [Google Scholar]
  18. Cowley JA, Hall MR, Cadogan LC, Spann KM, Walker PJ. Vertical transmission of gill-associated virus (GAV) in the black tiger prawn Penaeus monodon. Dis Aquat Organ 2002; 50: 95– 104 [CrossRef] [PubMed]
    [Google Scholar]
  19. Zg L, Zhang CS, Fh L, Xiang JH. Histological study on the gonadal development of Exopalaemon carinicauda (Holthuis, 1950). J Fisheries China 2014; 38: 362– 370
    [Google Scholar]
  20. Bell TA, Lightner DV. A Handbook of Normal Penaeid Shrimp Histology Baton Rouge, LA: World Aquaculture Society; 1988
    [Google Scholar]
  21. Lightner DV. A Handbook of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultured Penaeid Shrimp Baton Rouge, LA: World Aquaculture Society; 1996
    [Google Scholar]
  22. Piette D, Hendrickx M, Willems E, Kemp CR, Leyns L. An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies. Nat Protoc 2008; 3: 1194– 1201 [CrossRef] [PubMed]
    [Google Scholar]
  23. Chen S, Zhang G, Shao C, Huang Q, Liu G et al. Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle. Nat Genet 2014; 46: 253– 260 [CrossRef] [PubMed]
    [Google Scholar]
  24. Graham L, Orenstein JM. Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research. Nat Protoc 2007; 2: 2439– 2450 [CrossRef] [PubMed]
    [Google Scholar]
  25. Panphut W, Senapin S, Sriurairatana S, Withyachumnarnkul B, Flegel TW. A novel integrase-containing element may interact with Laem–Singh virus (LSNV) to cause slow growth in giant tiger shrimp. BMC Vet Res 2011; 7: 18 [CrossRef] [PubMed]
    [Google Scholar]
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