1887

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Volume 98, Issue 4

Anti-melanization mechanism of the white spot syndrome viral protein, WSSV453, via interaction with shrimp proPO-activating enzyme, proPPAE2 Free

Abstract

Inhibition of the host melanization reaction, activated by the prophenoloxidase activating (proPO) system, is one of the crucial evasion strategies of pathogens. Recently, the shrimp pathogen, white spot syndrome virus (WSSV), was found to inhibit melanization in the shrimp . The viral protein WSSV453 was previously shown to interact with PO-activating enzyme 2 (PPAE2) and reported to be involved in suppressing the shrimp melanization response after WSSV infection. Here, we characterized how WSSV453 inhibits melanization. WSSV453 is a non-structural viral protein, which was first detected in shrimp haemocytes at 6 hours post-infection (hpi) by WSSV and in shrimp plasma at 24 hpi. We produced recombinant proteins for three components of the proPO system: proPPAE2, proPO1 and proPO2. Functional assays showed that active PPAE2 processed proPO1 and 2 to produce functional PO. Incubation of WSSV453 with proPPAE2 dose-dependently reduced PPAE2 activity toward PO1 or PO2. In contrast, WSSV453 had no effect on activated PPAE2. The addition of active PPAE2 to WSSV-infected shrimp plasma at day 2 post-infection also rescued PO activity. Taken together, these results indicate that the anti-melanization activity of WSSV is due to WSSV453, which interacts with proPPAE2 and interferes with its activation to active PPAE2.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): melanization , Peneaus monodon , prophenoloxidase and white spot syndrome virus
© 2017 The Authors
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2017-04-01
2024-04-18
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Anti-melanization mechanism of the white spot syndrome viral protein, WSSV453, via interaction with shrimp proPO-activating enzyme, PmproPPAE2
J. Gen. Virol. 98, 769 (2017); https://doi.org/10.1099/jgv.0.000729
/content/journal/jgv/10.1099/jgv.0.000729
/content/journal/jgv/10.1099/jgv.0.000729
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