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Journal of General Virology header logo Journal of General Virology

Volume 98, Issue 4

155R is a novel structural protein of bovine adenovirus type 3, but it is not essential for virus replication No Access

Abstract

Bovine adenovirus (AdV) type 3 (BAdV-3) E1 region shares functional homology with E1 of human AdV type C5. Sequence analysis of the BAdV-3 E1 region revealed the presence of a novel 155R ORF that is not observed in other AdVs, on the lower strand antiparallel to a portion of the E1B region. The 155R gene products in BAdV-3-infected cells were identified by Northern blot, reverse transcriptase PCR followed by sequencing and Western blot analysis using the155R-specific antibody. 155R seems to be a late protein and is present in purified BAdV-3 particles. Replication kinetics of BAdV mutants with either one (BAdV/155R/mt1) or two (BAdV/155R/mt2) stop codons in the 155R ORF were comparable to those of BAdV-3, indicating that 155R is not essential for virus replication in cell culture. These results suggest that 155R-deleted BAdV-3 vectors could be generated in a cell line that fully complements BAdV-3 E1 functions.

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Keyword(s): 155R protein , adenoviral novel structural protein and bovine adenovirus type 3
© 2017 Purdue University
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/content/journal/jgv/10.1099/jgv.0.000707
2017-04-01
2025-04-19
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/content/journal/jgv/10.1099/jgv.0.000707
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155R is a novel structural protein of bovine adenovirus type 3, but it is not essential for virus replication
J. Gen. Virol. 98, 749 (2017); https://doi.org/10.1099/jgv.0.000707
/content/journal/jgv/10.1099/jgv.0.000707
/content/journal/jgv/10.1099/jgv.0.000707
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