@article{mbs:/content/journal/jgv/10.1099/jgv.0.000688, author = "Esona, Mathew D and Roy, Sunando and Rungsrisuriyachai, Kunchala and Sanchez, Jacqueline and Vasquez, Lina and Gomez, Virgen and Rios, Lourdes Aviles and Bowen, Michael D and Vazquez, Marietta", title = "Characterization of a triple-recombinant, reassortant rotavirus strain from the Dominican Republic", journal= "Journal of General Virology", year = "2017", volume = "98", number = "2", pages = "134-142", doi = "https://doi.org/10.1099/jgv.0.000688", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000688", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "rotavirus", keywords = "reassortment", keywords = "triple recombinant", abstract = "We report the genome of a novel human triple-recombinant G4P[6–8_R] mono-reassortant strain identified in a stool sample from the Dominican Republic during routine facility-based rotavirus strain surveillance. The strain was designated as RVA/Human-wt/DOM/2013840364/2013/G4P[6–8_R], with a genomic constellation of G4-P[6–8_R]-I1-R1-C1-M1-(A1-A8_R)-N1-(T1-T7_R)-E1-H1. Recombinant gene segments NSP1 and NSP3 were generated as a result of recombination between genogroup 1 rotavirus A1 human strain and a genotype A8 porcine strain and between genogroup 1 rotavirus T1 human strain and a genotype T7 bovine strain, respectively. Analyses of the RNA secondary structures of gene segment VP4, NSP1 and NSP3 showed that all the recombinant regions appear to start in a loop (single-stranded) region and terminate in a stem (double-stranded) structure. Also, the VP7 gene occupied lineage VII within the G4 genotypes consisting of mostly porcine or porcine-like G4 strains, suggesting the occurrence of reassortment. The remaining gene segments clustered phylogenetically with genogroup 1 strains. This exchange of whole or partial genetic materials between rotaviruses by recombination and reassortment contributes directly to their diversification, adaptation and evolution.", }