1887

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Volume 98, Issue 1

Interleukin-1 receptor antagonist: an early immunomodulatory cytokine induced by porcine reproductive and respiratory syndrome virus Free

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) infection poorly induces pro-inflammatory cytokines (IL-1, IL-6 and TNF-α) and type I IFN production during the early phase of infection. Our microarray analysis indicated strong upregulation of the gene in type 2 PRRSV -infected monocyte-derived dendritic cells. Interleukin-1 receptor antagonist (IL-1Ra) is an early inhibitory cytokine that suppresses pro-inflammatory cytokines and T-lymphocyte responses. To investigate the induction of IL-1Ra by PRRSV, monocyte-derived dendritic cells were cultured with type 2 PRRSV or other swine viruses. PRRSV increased both gene expression and IL-1Ra protein production in the culture. The enhanced production of IL-1Ra was further confirmed in PRRSV-cultured PBMC and PRRSV-exposed pigs by flow cytometry. Myeloid cell population appeared to be the major IL-1Ra producer both and . In contrast to the type 2 PRRSV, the highly pathogenic (HP)- PRRSV did not upregulate gene expression . To determine the kinetics of PRRSV-induced gene expression in relation to other pro-inflammatory cytokine genes, PRRSV-negative pigs were vaccinated with a commercially available type 2 modified-live PRRS vaccine or intranasally inoculated with HP-PRRSV. In modified-live PRRS vaccine pigs, upregulation of , but not and , gene expression was observed from 2 days post- vaccination. Consistent with the findings, upregulation of gene expression was not observed in the HP-PRRSV-infected pigs throughout the experiment. This study identified IL-1Ra as an early immunomodulatory mediator that could be involved in the immunopathogenesis of PRRSV infections.

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  • Accepted:
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Keyword(s): immunomodulatory cytokine , Interleukin-1 receptor antagonist and PRRSV
© 2017 The Authors
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2017-01-01
2024-04-25
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Interleukin-1 receptor antagonist: an early immunomodulatory cytokine induced by porcine reproductive and respiratory syndrome virus
J. Gen. Virol. 98, 77 (2017); https://doi.org/10.1099/jgv.0.000665
/content/journal/jgv/10.1099/jgv.0.000665
/content/journal/jgv/10.1099/jgv.0.000665
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