1887

Abstract

A disease outbreak was reported in adult koi, Cyprinus carpio koi, from a fish farm in Kerala, India, during June 2015. The clinical signs were observed only in recently introduced adult koi, and an existing population of fish did not show any clinical signs or mortality. Microscopic examination of wet mounts from the gills of affected koi revealed minor infestation of Dactylogyrus sp. in a few koi. In bacteriological studies, only opportunistic bacteria were isolated from the gills of affected fish. The histopathological examination of the affected fish revealed necrotic changes in gills and, importantly, virus particles were demonstrated in cytoplasm of gill epithelial cells in transmission electron microscopy. The tissue samples from affected koi were negative for common viruses reported from koi viz. cyprinid herpesvirus 3, spring viraemia of carp virus, koi ranavirus and red sea bream iridovirus in PCR screening. However, gill tissue from affected koi carp was positive for carp edema virus (CEV) in the first step of nested PCR, and sequencing of PCR amplicons confirmed infection with CEV. No cytopathic effect was observed in six fish cell lines following inoculation of filtered tissue homogenate prepared from gills of affected fish. In bioassay, the symptoms could be reproduced by inoculation of naive koi with filtrate from gill tissue homogenate of CEV-positive fish. Subsequently, screening of koi showing clinical signs similar to koi sleepy disease from different locations revealed that CEV infection was widespread. To our knowledge, this is the first report of infection with CEV in koi from India.

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2016-12-16
2019-10-23
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References

  1. Adamek M., Jung-Schroers V., Hellmann J., Teitge F., Bergmann S. M., Runge M., Kleingeld D. W., Way K., Stone D. M., Steinhagen D..( 2016;). Concentration of carp edema virus (CEV) DNA in koi tissues affected by koi sleepy disease (KSD). . Dis Aquat Organ 119: 245–251. [CrossRef] [PubMed]
    [Google Scholar]
  2. Amita K., Oe M., Matoyama H., Yamaguchi N., Fukuda H..( 2002;). A survey of koi herpesvirus and carp edema virus in color carp cultured in Niigata Prefecture, Japan. . Fish Pathol 37: 197–198. [CrossRef]
    [Google Scholar]
  3. Ariel E..( 2005;). Ornamental fish as trans-boundary vectors of viral diseases. . In Diseases in Asian Aquaculture, pp. 103–112. Edited by Walker P., Lester R., Bondad-Reantaso M. G.. Manila:: Fish Health Section, Asian Fisheries Society;.
    [Google Scholar]
  4. Bercovier H., Fishman Y., Nahary R., Sinai S., Zlotkin A., Eyngor M., Gilad O., Eldar A., Hedrick R. P..( 2005;). Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis. . BMC Microbiol 5: 13. [CrossRef] [PubMed]
    [Google Scholar]
  5. George M. R., John K. R., Mansoor M. M., Saravanakumar R., Sundar P., Pradeep V..( 2015;). Isolation and characterization of a ranavirus from koi, Cyprinus carpio L., experiencing mass mortalities in India. . J Fish Dis 38: 389–403. [CrossRef] [PubMed]
    [Google Scholar]
  6. Haenen O., Way K., Stone D., Engelsma M..( 2013;). [Koi sleepy disease found for the first time in koi carps in The Netherlands]. . Tijdschr Diergeneeskd 5: 27–29 (in Dutch).
    [Google Scholar]
  7. Jung-Schroers V., Adamek M., Teitge F., Hellmann J., Bergmann S. M., Schütze H., Kleingeld D. W., Way K., Stone D. et al.( 2015;). Another potential carp killer? Carp edema virus disease in Germany. . BMC Vet Res 11: 114. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kurita J., Nakajima K., Hirono I., Aoki T..( 1998;). Polymerase chain reaction (PCR) amplification of DNA of red sea bream iridovirus (RSIV). . Fish Pathol 33: 17–23. [CrossRef]
    [Google Scholar]
  9. Lewisch E., Gorgoglione B., Way K., El-Matbouli M..( 2015;). Carp edema virus/koi sleepy disease: an emerging disease in Central-East Europe. . Transbound Emerg Dis 62: 6–12. [CrossRef] [PubMed]
    [Google Scholar]
  10. Magnadottir B..( 2010;). Immunological control of fish diseases. . Mar Biotechnol 12: 361–379. [CrossRef] [PubMed]
    [Google Scholar]
  11. Matras M., Borzym E., Stone D., Way K., Stachnik M., Maj-Paluch J., Palusińska M., Reichert M..( 2016;). Carp edema virus in Polish aquaculture — evidence of significant sequence divergence and a new lineage in common carp Cyprinus carpio (L.). . J Fish Dis. [CrossRef]
    [Google Scholar]
  12. Miyazaki T., Isshiki T., Katsuyuki H..( 2005;). Histopathological and electron microscopy studies on sleepy disease of koi Cyprinus carpio koi in Japan. . Dis Aquat Organ 65: 197–207. [CrossRef] [PubMed]
    [Google Scholar]
  13. Murakami Y., Shitanaka M., Toshida S., Matsuzato T..( 1976;). [Studies on mass mortality of juvenile carp: about mass mortality showing oedema]. . Bull Hiroshima Fresh Water Fish Exp Stn 19–33 (in Japanese).
    [Google Scholar]
  14. Ono S., Nagai A., Sugai N..( 1986;). A histopathological study on juvenile colorcarp, Cyprinus carpio, showing edema. . Fish Pathol 21: 167–175.[CrossRef]
    [Google Scholar]
  15. Oyamatsu T., Hata N., Yamada K., Sano T., Fukuda H..( 1997a;). An etiological study on mass mortality of cultured colour carp juveniles showing edema. . Fish Pathol 32: 81–88. [CrossRef]
    [Google Scholar]
  16. Oyamatsu T., Matoyama H., Yamamoto K., Fukuda H..( 1997b;). A trial for detection of carp edema virus by using polymerase chain reaction. . Aqua Sci 45: 247–251.
    [Google Scholar]
  17. Pretto T., Abbadi M., Panzarin V., Quartesan R., Manfrin A., Toffan A..( 2015;). Carp edema virus (CEV): first detection in Italy. . 17th International Conference on Diseases of Fish and Shellfish, Abstract and poster, p. 343. Las Palmas, Gran Canaria, Organized by European Association of Fish Pathologists.
    [Google Scholar]
  18. Stone D. M., Ahne W., Denham K. L., Dixon P. F., Liu C. T., Sheppard A. M., Taylor G. R., Way K..( 2003;). Nucleotide sequence analysis of the glycoprotein gene of putative spring viraemia of carp virus and pike fry rhabdovirus isolates reveals four genogroups. . Dis Aquat Organ 53: 203–210. [CrossRef] [PubMed]
    [Google Scholar]
  19. Swaminathan T. R., Lakra W. S., Gopalakrishnan A., Basheer V. S., Khushwaha B., Sajeela K. A..( 2010;). Development and characterization of a new epithelial cell line PSF from caudal fin of green chromide, Etroplus suratensis (Bloch, 1790). . In Vitro Cell Dev Biol Anim 46: 647–656. [CrossRef] [PubMed]
    [Google Scholar]
  20. Swaminathan T. R., Basheer V. S., Gopalakrishnan A., Rathore G., Chaudhary D. K., Kumar R., Jena J. K..( 2013;). Establishment of caudal fin cell lines from tropical ornamental fish Puntius fasciatus and Pristolepis fasciata endemic to the Western Ghats of India. . Acta Trop 128: 536–541. [CrossRef] [PubMed]
    [Google Scholar]
  21. Swaminathan T. R., Basheer V. S., Kumar R., Kathirvelpandian A., Sood N., Jena J. K..( 2015;). Establishment and characterization of fin-derived cell line from ornamental carp, Cyprinus carpio koi, for virus isolation in India. . In Vitro Cell Dev Biol Anim 51: 705–713. [CrossRef] [PubMed]
    [Google Scholar]
  22. Swaminathan T. R., Basheer V. S., Gopalakrishnan A., Sood N., Pradhan P. K..( 2016a;). A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864). . Cytotechnology 68: 515–523. [CrossRef] [PubMed]
    [Google Scholar]
  23. Swaminathan T. R., Kumar R., Jency P. M., Charan R., Syamkrishnan M. U., Basheer V. S., Sood N., Jena J. K..( 2016b;). A new fish cell line derived from the caudal fin of freshwater angelfish Pterophyllum scalare: development and characterization. . J Fish Biol 89: 1769–1781. [CrossRef]
    [Google Scholar]
  24. Tamura K., Dudley J., Nei M., Kumar S..( 2007;). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. . Mol Biol Evol 24: 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  25. Vesely T., Pokorova D., Reschova S., Piackova V..( 2015;). Detection of carp edema virus in common carp (Cyprinus carpio) and koi in the Czech Republic. . In 17th International Conference on Diseases of Fish and Shellfish, Abstract and poster, p. 346. Las Palmas, Gran Canaria, Organized by European Association of Fish Pathologists.
    [Google Scholar]
  26. Way K., Stone D..( 2013;). Emergence of carp edema virus-like (CEV-like) disease in the UK. . CEFAS Finfish News 15: 32–34.
    [Google Scholar]
  27. Whittington R. J., Chong R..( 2007;). Global trade in ornamental fish from an Australian perspective: the case for revised import risk analysis and management strategies. . Prev Vet Med 81: 92–116. [CrossRef] [PubMed]
    [Google Scholar]
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