1887

Abstract

In this study we investigated if human umbilical cord blood serum (CBS) is a suitable replacement for foetal bovine serum (FBS) in cultures of human hepatoma cell line Huh7.5, particularly regarding its capacity to maintain high growth rates, differentiation status and its ability to support robust hepatitis C virus (HCV) infection. Generally, CBS-cultured Huh7.5 cells remained comparable to FBS-cultured cells, and proliferated equally well. Albumin secretion, a hepatocyte differentiation marker, had increased 8x in CBS; however, most other hepatocyte markers we tested had not changed. Surprisingly, CBS-cultured cells were able to sustain very high levels of HCV production, and HCV infection in CBS-cultured cells did not induce cell lysis, which is typically seen in HCV-infected cells cultured in FBS. We discuss some of the differences between CBS, adult human serum and FBS that may explain the differences observed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000636
2016-12-16
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/97/12/3248.html?itemId=/content/journal/jgv/10.1099/jgv.0.000636&mimeType=html&fmt=ahah

References

  1. Ang L. P., Do T. P., Thein Z. M., Reza H. M., Tan X. W., Yap C., Tan D. T., Beuerman R. W. 2011; Ex vivo expansion of conjunctival and limbal epithelial cells using cord blood serum-supplemented culture medium. Invest Ophthalmol Vis Sci 52:6138–6147 [View Article][PubMed]
    [Google Scholar]
  2. Ayatollahi M., Soleimani M., Geramizadeh B., Imanieh M. H. 2011; Insulin-like growth factor 1 (IGF-I) improves hepatic differentiation of human bone marrow-derived mesenchymal stem cells. Cell Biol Int 35:1169–1176 [View Article][PubMed]
    [Google Scholar]
  3. Böhm F., Köhler U. A., Speicher T., Werner S. 2010; Regulation of liver regeneration by growth factors and cytokines. EMBO Mol Med 2:294–305 [View Article][PubMed]
    [Google Scholar]
  4. Lindenbach B. D., Meuleman P., Ploss A., Vanwolleghem T., Syder A. J., McKeating J. A., Lanford R. E., Feinstone S. M., Major M. E. et al. 2006; Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro . Proc Natl Acad Sci U S A 103:3805–3809 [View Article][PubMed]
    [Google Scholar]
  5. Mallon B. S., Park K. Y., Chen K. G., Hamilton R. S., McKay R. D. 2006; Toward xeno-free culture of human embryonic stem cells. Int J Biochem Cell Biol 38:1063–1075 [View Article][PubMed]
    [Google Scholar]
  6. Michalopoulos G. K. 2007; Liver regeneration. J Cell Physiol 213:286–300 [View Article][PubMed]
    [Google Scholar]
  7. Nakamura T., Sakai K., Nakamura T., Matsumoto K. 2011; Hepatocyte growth factor twenty years on: much more than a growth factor. J Gastroenterol Hepatol 26:188–202 [View Article][PubMed]
    [Google Scholar]
  8. Navone S. E., Marfia G., Nava S., Invernici G., Cristini S., Balbi S., Sangiorgi S., Ciusani E., Bosutti A. et al. 2013; Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival. Vasc Cell 5:10 [View Article][PubMed]
    [Google Scholar]
  9. Ornitz D. M., Itoh N. 2001; Fibroblast growth factors. Genome Biol 2:REVIEWS3005[PubMed] [CrossRef]
    [Google Scholar]
  10. Sainz B., Chisari F. V. 2006; Production of infectious hepatitis C virus by well-differentiated, growth-arrested human hepatoma-derived cells. J Virol 80:10253–10257 [View Article][PubMed]
    [Google Scholar]
  11. Shetty P., Bharucha K., Tanavde V. 2007; Human umbilical cord blood serum can replace fetal bovine serum in the culture of mesenchymal stem cells. Cell Biol Int 31:293–298 [View Article][PubMed]
    [Google Scholar]
  12. Steenbergen R. H., Joyce M. A., Thomas B. S., Jones D., Law J., Russell R., Houghton M., Tyrrell D. L. 2013; Human serum leads to differentiation of human hepatoma cells, restoration of VLDL secretion and a 1000-fold increase in HCV JFH-1 titers. Hepatology 58:1907–1917 [CrossRef]
    [Google Scholar]
  13. Steiling H., Wüstefeld T., Bugnon P., Brauchle M., Fässler R., Teupser D., Thiery J., Gordon J. I., Trautwein C., Werner S. 2003; Fibroblast growth factor receptor signalling is crucial for liver homeostasis and regeneration. Oncogene 22:4380–4388 [View Article][PubMed]
    [Google Scholar]
  14. Taub R. 2004; Liver regeneration: from myth to mechanism. Nat Rev Mol Cell Biol 5:836–847 [View Article][PubMed]
    [Google Scholar]
  15. Vajpayee R. B., Mukerji N., Tandon R., Sharma N., Pandey R. M., Biswas N. R., Malhotra N., Melki S. A. 2003; Evaluation of umbilical cord serum therapy for persistent corneal epithelial defects. Br J Ophthalmol 87:1312–1316 [View Article][PubMed]
    [Google Scholar]
  16. Vander Heiden M. G., Cantley L. C., Thompson C. B. 2009; Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science 324:1029–1033 [View Article][PubMed]
    [Google Scholar]
  17. Wakita T., Pietschmann T., Kato T., Date T., Miyamoto M., Zhao Z., Murthy K., Habermann A., Kräusslich H. G. et al. 2005; Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nat Med 11:791–796 [View Article][PubMed]
    [Google Scholar]
  18. Xia D., He H. Y., Lei Z. M., Zhang P. M., Guo Y. 2004; Effects of human umbilical cord serum on proliferation and insulin content of human fetal islet-like cell clusters. Hepatobiliary Pancreat Dis Int 3:144–148[PubMed]
    [Google Scholar]
  19. Yoon K. C., Jeong I. Y., Im S. K., Park Y. G., Kim H. J., Choi J. 2007a; Therapeutic effect of umbilical cord serum eyedrops for the treatment of dry eye associated with graft-versus-host disease. Bone Marrow Transplant 39:231–235 [View Article][PubMed]
    [Google Scholar]
  20. Yoon K. C., You I. C., Im S. K., Jeong T. S., Park Y. G., Choi J. 2007b; Application of umbilical cord serum eyedrops for the treatment of neurotrophic keratitis. Ophthalmology 114:1637–1642 [View Article][PubMed]
    [Google Scholar]
  21. Zhong J., Gastaminza P., Cheng G., Kapadia S., Kato T., Burton D. R., Wieland S. F., Uprichard S. L., Wakita T., Chisari F. V. 2005; Robust hepatitis C virus infection in vitro . Proc Natl Acad Sci U S A 102:9294–9299 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000636
Loading
/content/journal/jgv/10.1099/jgv.0.000636
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error