1887

Journal of General Virology header logo

Volume 97, Issue 12

Genetic mutations in live infectious bronchitis vaccine viruses following single or dual infection of tracheal organ cultures Free

Abstract

Despite regular co-vaccination of two different strains of live infectious bronchitis vaccine viruses, little is known about possible mutations in these viruses following vaccination. As an alternative to chicks, this study used an infection model to identify single-nucleotide polymorphisms (SNPs) within the part-S1 gene of two live infectious bronchitis virus vaccine strains (793B and Massachusetts) following single or dual inoculation onto tracheal organ cultures. Results indicate that viral titres reduced over the duration of the study; conversely, the amount of detected infectious bronchitis virus genome increased. Results demonstrate a greater number of non-synonymous SNPs in both vaccine strains when they are co-inoculated, compared with the single inoculations. The influence of the increased SNP and hydrophobic properties of the translated proteins on the vaccine viruses’ virulence is unknown.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): amino acid , Infectious bronchitis vaccine viruses , single nucleotide polymorphism and tracheal organ culture
© 2016 The Authors
Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000628
2016-12-16
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/97/12/3232.html?itemId=/content/journal/jgv/10.1099/jgv.0.000628&mimeType=html&fmt=ahah

References

  1. Abolnik C. 2015; Genomic and single nucleotide polymorphism analysis of infectious bronchitis coronavirus. Infect Genet Evol 32:416–424 [View Article][PubMed]
     [Google Scholar]
  2. Awad F., Baylis M., Ganapathy K. 2014; Detection of variant infectious bronchitis viruses in broiler flocks in Libya. Int J Vet Sci Med 2:78–82 [View Article]
     [Google Scholar]
  3. Awad F., Forrester A., Baylis M., Lemiere S., Jones R., Ganapathy K. 2015; Immune responses and interactions following simultaneous application of live Newcastle disease, infectious bronchitis and avian metapneumovirus vaccines in specific-pathogen-free chicks. Res Vet Sci 98:127–133 [View Article][PubMed]
     [Google Scholar]
  4. Awad F., Hutton S., Forrester A., Baylis M., Ganapathy K. 2016; Heterologous live infectious bronchitis virus vaccination in day-old commercial broiler chicks: clinical signs, ciliary health, immune responses and protection against variant infectious bronchitis viruses. Avian Pathol 45:169–177 [View Article][PubMed]
     [Google Scholar]
  5. Cavanagh D., Davis P. J., Mockett A. P. 1988; Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes. Virus Res 11:141–191 [View Article][PubMed]
     [Google Scholar]
  6. Cavanagh D., Mawditt K., Britton P., Naylor C. J. 1999; Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broilers using type-specific polymerase chain reactions. Avian Pathol 28:593–605 [View Article]
     [Google Scholar]
  7. Cavanagh D., Picault J. P., Gough R., Hess M., Mawditt K., Britton P. 2005; Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs. Avian Pathol 34:20–25 [View Article][PubMed]
     [Google Scholar]
  8. Chhabra R., Forrester A., Lemiere S., Awad F., Chantrey J., Ganapathy K. 2015; Mucosal, cellular, and humoral immune responses induced by different live infectious bronchitis virus vaccination regimes and protection conferred against infectious bronchitis virus Q1 strain. Clin Vaccine Immunol 22:1050–1059 [View Article][PubMed]
     [Google Scholar]
  9. Cook J. K., Darbyshire J. H., Peters R. W. 1976; The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus. Arch Virol 50:109–118 [View Article][PubMed]
     [Google Scholar]
  10. Cook J. K., Ellis M. M., Dolby C. A., Holmes H. C., Finney P. M., Huggins M. B. 1989; A live attenuated turkey rhinotracheitis virus vaccine. 1. Stability of the attenuated strain. Avian Pathol 18:511–522 [View Article][PubMed]
     [Google Scholar]
  11. Cook J. K., Huggins M. B., Orbell S. J., Mawditt K., Cavanagh D. 2001; Infectious bronchitis virus vaccine interferes with the replication of avian pneumovirus vaccine in domestic fowl. Avian Pathol 30:233–242 [View Article][PubMed]
     [Google Scholar]
  12. De Wit J. J., Brandao P., Torres C. A., Koopman R., Villarreal L. Y. 2015; Increased level of protection of respiratory tract and kidney by combining different infectious bronchitis virus vaccines against challenge with nephropathogenic Brazilian genotype subcluster 4 strains. Avian Pathol 44:352–357 [View Article][PubMed]
     [Google Scholar]
  13. Dolz R., Pujols J., Ordóñez G., Porta R., Majó N. 2008; Molecular epidemiology and evolution of avian infectious bronchitis virus in Spain over a fourteen-year period. Virology 374:50–59 [View Article]
     [Google Scholar]
  14. Domingo E., Holland J. J. 1997; RNA virus mutations and fitness for survival. Annu Rev Microbiol 51:151–178 [View Article][PubMed]
     [Google Scholar]
  15. Ducatez M. F., Martin A. M., Owoade A. A., Olatoye I. O., Alkali B. R., Maikano I., Snoeck C. J., Sausy A., Cordioli P., Muller C. P. 2009; Characterization of a new genotype and serotype of infectious bronchitis virus in Western Africa. J Gen Virol 90:2679–2685 [View Article][PubMed]
     [Google Scholar]
  16. Ganapathy K., Cox W. J., Gough R. E., Cargill P., Montiel E., Jones R. C. 2007; Protection in specific pathogen free chickens with live avian metapneumovirus and Newcastle disease virus vaccines applied singly or in combination. Avian Pathol 36:313–317 [View Article][PubMed]
     [Google Scholar]
  17. Ganapathy K., Ball C., Forrester A. 2015; Genotypes of infectious bronchitis viruses circulating in the Middle East between 2009 and 2014. Virus Res 210:198–204 [View Article][PubMed]
     [Google Scholar]
  18. Hodgson T., Casais R., Dove B., Britton P., Cavanagh D. 2004; Recombinant infectious bronchitis coronavirus Beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity. J Virol 78:13804–13811 [View Article][PubMed]
     [Google Scholar]
  19. Jackwood M. W. 2012; Review of infectious bronchitis virus around the world. Avian Dis 56:634–641 [View Article][PubMed]
     [Google Scholar]
  20. Jones R. C. 2010; Viral respiratory diseases (ILT, aMPV infections, IB): are they ever under control?. Br Poult Sci 51:1–11 [View Article][PubMed]
     [Google Scholar]
  21. Jones R. M., Ellis R. J., Cox W. J., Errington J., Fuller C., Irvine R. M., Wakeley P. R. 2011; Development and validation of RT-PCR tests for the detection and S1 genotyping of infectious bronchitis virus and other closely related gammacoronaviruses within clinical samples. Transbound Emerg Dis 58:411–420 [View Article][PubMed]
     [Google Scholar]
  22. Lai M. M., Cavanagh D. 1997; The molecular biology of coronaviruses. Adv Virus Res 48:1–100[PubMed] [CrossRef]
     [Google Scholar]
  23. Löndt B. Z., Brookes S. M., Kelly M. D., Nash B. J., Brown I. H. 2013; Failure to infect pigs co-housed with ducks or chickens infected experimentally with A/turkey/Turkey/1/2005 (H5N1) highly pathogenic avian influenza virus. Vet Microbiol 162:944–948 [View Article][PubMed]
     [Google Scholar]
  24. Raj G. D., Jones R. C. 1996; An in vitro comparison of the virulence of seven strains of infectious bronchitis virus using tracheal and oviduct organ cultures. Avian Pathol 25:649–662 [View Article][PubMed]
     [Google Scholar]
  25. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. Am J Epidemiol 27:493–497
     [Google Scholar]
  26. Roh H. J., Hilt D. A., Williams S. M., Jackwooda M. W. 2013; Evaluation of infectious bronchitis virus Arkansas-type vaccine failure in commercial broilers. Avian Dis 57:248–259 [View Article][PubMed]
     [Google Scholar]
  27. Roh H. J., Hilt D. A., Jackwood M. W. 2014; Detection of infectious bronchitis virus with the use of real-time quantitative reverse transcriptase-PCR and correlation with virus detection in embryonated eggs. Avian Dis 58:398–403 [View Article][PubMed]
     [Google Scholar]
  28. Schalk A., Hawn M. 1931; An apparently new respiratory disease of baby chicks. J Am Vet Med Assoc 78:413–422
     [Google Scholar]
  29. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  30. van Santen V. L., Toro H. 2008; Rapid selection in chickens of subpopulations within ArkDPI-derived infectious bronchitis virus vaccines. Avian Pathol 37:293–306 [View Article][PubMed]
     [Google Scholar]
  31. Villarreal L. Y., Sandri T. L., Souza S. P., Richtzenhain L. J., de Wit J. J., Brandao P. E. 2010; Molecular epidemiology of avian infectious bronchitis in Brazil from 2007 to 2008 in breeders, broilers, and layers. Avian Dis 54:894–898 [View Article][PubMed]
     [Google Scholar]
  32. Wang H. N., Wu Q. Z., Huang Y., Liu P. 1997; Isolation and identification of infectious bronchitis virus from chickens in Sichuan, China. Avian Dis 41:279–282 [View Article][PubMed]
     [Google Scholar]
  33. Winter C., Herrler G., Neumann U. 2008; Infection of the tracheal epithelium by infectious bronchitis virus is sialic acid dependent. Microbes Infect 10:367–373 [View Article][PubMed]
     [Google Scholar]
  34. Worthington K. J., Currie R. J., Jones R. C. 2008; A reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006. Avian Pathol 37:247–257 [View Article][PubMed]
     [Google Scholar]
  35. Zhao F., Han Z., Zhang T., Shao Y., Kong X., Ma H., Liu S. 2014; Genomic characteristics and changes of avian infectious bronchitis virus strain CK/CH/LDL/97I after serial passages in chicken embryos. Intervirology 57:319–330 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000628
Loading
Genetic mutations in live infectious bronchitis vaccine viruses following single or dual in vitro infection of tracheal organ cultures
J. Gen. Virol. 97, 3232 (2016); https://doi.org/10.1099/jgv.0.000628
/content/journal/jgv/10.1099/jgv.0.000628
/content/journal/jgv/10.1099/jgv.0.000628
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error