1887

Abstract

Group A rotavirus is a major cause of diarrhoea in humans, especially in young children. Bats also harbour group A rotaviruses, but the genetic backgrounds of bat rotavirus strains are usually distinct from those of human rotavirus strains. We identified a new strain of group A rotavirus in the intestinal contents of a horseshoe bat in Zambia. Whole genome sequencing revealed that the identified virus, named RVA/Bat-wt/ZMB/LUS12-14/2012/G3P[3], possessed the genotype constellation G3-P[3]-I3-R2-C2-M3-A9-N2-T3-E2-H3. Several genome segments of LUS12-14 were highly similar to those of group A rotaviruses identified from humans, cows and antelopes, indicating interspecies transmission of rotaviruses between bats and other mammals with possible multiple genomic reassortment events.

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2016-10-13
2020-04-04
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References

  1. Beres L. K., Tate J. E., Njobvu L., Chibwe B., Rudd C., Guffey M. B., Stringer J. S., Parashar U. D., Chilengi R.. 2016; A preliminary assessment of rotavirus vaccine effectiveness in Zambia. Clin Infect Dis62:S175–S182 [CrossRef][PubMed]
    [Google Scholar]
  2. Darissa O., Willingmann P., Adam G.. 2010; Optimized approaches for the sequence determination of double-stranded RNA templates. J Virol Methods169:397–403 [CrossRef][PubMed]
    [Google Scholar]
  3. De Grazia S., Martella V., Giammanco G. M., Gòmara M. I., Ramirez S., Cascio A., Colomba C., Arista S.. 2007; Canine-origin G3P[3] rotavirus strain in child with acute gastroenteritis. Emerg Infect Dis13:1091–1093[CrossRef]
    [Google Scholar]
  4. Esona M. D., Mijatovic-Rustempasic S., Conrardy C., Tong S., Kuzmin I. V., Agwanda B., Breiman R. F., Banyai K., Niezgoda M. et al. 2010; Reassortant group A rotavirus from straw-colored fruit bat (Eidolon helvum). Emerg Infect Dis16:1844–1852[CrossRef]
    [Google Scholar]
  5. Fujii Y., Shimoike T., Takagi H., Murakami K., Todaka-Takai R., Park Y., Katayama K.. 2012; Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction. Microbiol Immunol56:630–638 [CrossRef][PubMed]
    [Google Scholar]
  6. Guo D., Liu J., Lu Y., Yuan D., Jiang Q., Lin H., Li C., Si C., Qu L.. 2012; Full genomic analysis of rabbit rotavirus G3P[14] strain N5 in China: identification of a novel VP6 genotype. Infect Genet Evol12:1567–1576[CrossRef]
    [Google Scholar]
  7. He B., Yang F., Yang W., Zhang Y., Feng Y., Zhou J., Xie J., Feng Y., Bao X. et al. 2013; Characterization of a novel G3P[3] rotavirus isolated from a lesser horseshoe bat: a distant relative of feline/canine rotaviruses. J Virol87:12357–12366 [CrossRef][PubMed]
    [Google Scholar]
  8. Jere K. C., Mlera L., O'Neill H. G., Peenze I., van Dijk A. A.. 2012; Whole genome sequence analyses of three African bovine rotaviruses reveal that they emerged through multiple reassortment events between rotaviruses from different mammalian species. Vet Microbiol159:245–250 [CrossRef][PubMed]
    [Google Scholar]
  9. Kumar S., Stecher G., Tamura K.. 2016; mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  10. Li K., Lin X. D., Huang K. Y., Zhang B., Shi M., Guo W. P., Wang M. R., Wang W., Xing J. G. et al. 2016; Identification of novel and diverse rotaviruses in rodents and insectivores, and evidence of cross-species transmission into humans. Virology494:168–177 [CrossRef][PubMed]
    [Google Scholar]
  11. Maes P., Matthijnssens J., Rahman M., Van Ranst M.. 2009; RotaC: a web-based tool for the complete genome classification of group A rotaviruses. BMC Microbiol9:238 [CrossRef][PubMed]
    [Google Scholar]
  12. Matthijnssens J., Ciarlet M., Rahman M., Attoui H., Bányai K., Estes M. K., Gentsch J. R., Iturriza-Gómara M., Kirkwood C. D. et al. 2008; Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol153:1621–1629 [CrossRef][PubMed]
    [Google Scholar]
  13. Matthijnssens J., Potgieter C. A., Ciarlet M., Parreño V., Martella V., Bányai K., Garaicoechea L., Palombo E. A., Novo L. et al. 2009; Are human P[14] rotavirus strains the result of interspecies transmissions from sheep or other ungulates that belong to the mammalian order Artiodactyla?. J Virol83:2917–2929 [CrossRef][PubMed]
    [Google Scholar]
  14. Matthijnssens J., Ciarlet M., McDonald S. M., Attoui H., Bányai K., Brister J. R., Buesa J., Esona M. D., Estes M. K. et al. 2011a; Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol156:1397–1413[CrossRef]
    [Google Scholar]
  15. Matthijnssens J., De Grazia S., Piessens J., Heylen E., Zeller M., Giammanco G. M., Bányai K., Buonavoglia C., Ciarlet M. et al. 2011b; Multiple reassortment and interspecies transmission events contribute to the diversity of feline, canine and feline/canine-like human group A rotavirus strains. Infect Genet Evol11:1396–1406[CrossRef]
    [Google Scholar]
  16. Mpabalwani E. M., Simwaka C. J., Mwenda J. M., Mubanga C. P., Monze M., Matapo B., Parashar U. D., Tate J. E.. 2016; Impact of rotavirus vaccination on diarrheal hospitalizations in children aged <5 years in Lusaka, Zambia. Clin Infect Dis62:S183–S187 [CrossRef][PubMed]
    [Google Scholar]
  17. Pongsuwanna Y., Guntapong R., Chiwakul M., Tacharoenmuang R., Onvimala N., Wakuda M., Kobayashi N., Taniguchi K.. 2002; Detection of a human rotavirus with G12 and P[9] specificity in Thailand. J Clin Microbiol40:1390–1394[PubMed][CrossRef]
    [Google Scholar]
  18. Ramig R. F.. 2007; Systemic rotavirus infection. Expert Rev Anti Infect Ther5:591–612 [CrossRef][PubMed]
    [Google Scholar]
  19. Sasaki M., Setiyono A., Handharyani E., Rahmadani I., Taha S., Adiani S., Subangkit M., Sawa H., Nakamura I., Kimura T.. 2012; Molecular detection of a novel paramyxovirus in fruit bats from Indonesia. Virol J9:240 [CrossRef][PubMed]
    [Google Scholar]
  20. Sasaki M., Orba Y., Ueno K., Ishii A., Moonga L., Hang'ombe B. M., Mweene A. S., Ito K., Sawa H.. 2015; Metagenomic analysis of the shrew enteric virome reveals novel viruses related to human stool-associated viruses. J Gen Virol96:440–452 [CrossRef][PubMed]
    [Google Scholar]
  21. Xia L., Fan Q., He B., Xu L., Zhang F., Hu T., Wang Y., Li N., Qiu W. et al. 2014; The complete genome sequence of a G3P[10] Chinese bat rotavirus suggests multiple bat rotavirus inter-host species transmission events. Infect Genet Evol28:1–4 [CrossRef][PubMed]
    [Google Scholar]
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