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Volume 97, Issue 10

Expanding the host range of small insect RNA viruses: () infects and replicates in a human tissue culture cell line Free

Abstract

Tetraviruses are small, positive (+ve)-sense ssRNA viruses that infect the midgut cells of lepidopteran larvae. (PrV) is the only member of the family (previously ). PrV particles exhibit the characteristic tetraviral =4 icosahedral symmetry, but PrV is distinct from other tetraviruses with respect to genome organization and viral non-structural proteins. Currently, PrV is the only tetravirus known to infect and replicate in lepidopteran cell culture lines. In this report we demonstrate, using immunofluorescence microscopy, that PrV infects and replicates in a human tissue culture cell line (HeLa), producing infectious virus particles. We also provide evidence for PrV replication in insect, mammalian and plant cell-free systems. This study challenges the long-held view that tetraviruses have a narrow host range confined to one or a few lepidopteran species and highlights the need to consider the potential for apparently non-infectious viruses to be transferred to new hosts in the laboratory.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Carmotetraviridae , cross-kingdom transfer , HeLa cell line and infection
© 2016 The Authors
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2016-10-13
2024-04-23
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Expanding the host range of small insect RNA viruses: Providence virus (Carmotetraviridae) infects and replicates in a human tissue culture cell line
J. Gen. Virol. 97, 2763 (2016); https://doi.org/10.1099/jgv.0.000578
/content/journal/jgv/10.1099/jgv.0.000578
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