1887

Abstract

Reoviruses (family ) infect vertebrate and invertebrate hosts with clinical effects ranging from inapparent to lethal. Here, we describe the discovery and characterization of (LMBRV), found during investigation of a mortality event in wild largemouth bass () in 2015 in WI, USA. LMBRV has spherical virions of approximately 80 nm diameter containing 10 segments of linear dsRNA, aligning it with members of the genus , which infect mammals and birds, rather than members of the genus , which contain 11 segments and infect teleost fishes. LMBRV is only between 24 % and 68 % similar at the amino acid level to its closest relative, (PRV), the putative cause of heart and skeletal muscle inflammation of farmed salmon. LMBRV expands the known diversity and host range of its lineage, which suggests that an undiscovered diversity of related pathogenic reoviruses may exist in wild fishes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000568
2016-10-13
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/97/10/2482.html?itemId=/content/journal/jgv/10.1099/jgv.0.000568&mimeType=html&fmt=ahah

References

  1. Abascal F., Zardoya R., Telford M. J. 2010; TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations. Nucleic Acids Res 38:W7–W13 [View Article][PubMed]
    [Google Scholar]
  2. Attoui H., Becnel J., Belaganahalli S., Bergoin M., Brussaard C., Chappell J., Ciarlet M., del Vas M., Dermody T. et al. 2012; Part II: The Viruses – the double stranded RNA viruses – family Reoviridae . In Virus Taxonomy: Classification and Nomenclature: Ninth Report of the International Committee on Taxonomy of Viruses pp. 541–637 Edited by King A. M. Q., Adams M. J., Lefowitz E. J. London, UK: Academic Press;
    [Google Scholar]
  3. Auguste A. J., Kaelber J. T., Fokam E. B., Guzman H., Carrington C. V., Erasmus J. H., Kamgang B., Popov V. L., Jakana J. et al. 2015; A newly isolated reovirus has the simplest genomic and structural organization of any reovirus. J Virol 89:676–687 [View Article][PubMed]
    [Google Scholar]
  4. Blindheim S., Nylund A., Watanabe K., Plarre H., Erstad B., Nylund S. 2015; A new aquareovirus causing high mortality in farmed Atlantic halibut fry in Norway. Arch Virol 160:91–102 [View Article][PubMed]
    [Google Scholar]
  5. Chen Z. Y., Gao X. C., Zhang Q. Y. 2015; Whole-genome analysis of a novel fish reovirus (MsRev) discloses aquareovirus genomic structure relationship with host in saline environments. Viruses 7:4282–4302 [View Article][PubMed]
    [Google Scholar]
  6. Dhama K., Chauhan R. S., Mahendran M., Malik S. V. 2009; Rotavirus diarrhea in bovines and other domestic animals. Vet Res Commun 33:1–23 [View Article][PubMed]
    [Google Scholar]
  7. Finstad O. W., Dahle M. K., Lindholm T. H., Nyman I. B., Løvoll M., Wallace C., Olsen C. M., Storset A. K., Rimstad E. 2014; Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes. Vet Res 45:35 [View Article][PubMed]
    [Google Scholar]
  8. Garseth A. H., Ekrem T., Biering E. 2013a; Phylogenetic evidence of long distance dispersal and transmission of piscine reovirus (PRV) between farmed and wild atlantic salmon. PLoS One 8:e82202 [CrossRef]
    [Google Scholar]
  9. Garseth Å. H., Fritsvold C., Opheim M., Skjerve E., Biering E. 2013b; Piscine reovirus (PRV) in wild Atlantic salmon, Salmo salar L., and sea-trout, Salmo trutta L., in Norway. J Fish Dis 36:483–493 [View Article]
    [Google Scholar]
  10. Garver K. A., Marty G. D., Cockburn S. N., Richard J., Hawley L. M., Müller A., Thompson R. L., Purcell M. K., Saksida S. 2016; Piscine reovirus, but not Jaundice syndrome, was transmissible to Chinook salmon, Oncorhynchus tshawytscha (Walbaum), Sockeye salmon, Oncorhynchus nerka (Walbaum), and Atlantic salmon, Salmo salar L. J Fish Dis 39:117–128 [View Article][PubMed]
    [Google Scholar]
  11. Katoh K., Misawa K., Kuma K., Miyata T. 2002; mafft: a novel method for rapid multiple sequence alignment based on fast fourier transform. Nucleic Acids Res 30:3059–3066 [View Article][PubMed]
    [Google Scholar]
  12. Kibenge M. J., Iwamoto T., Wang Y., Morton A., Godoy M. G., Kibenge F. S. 2013; Whole-genome analysis of piscine reovirus (PRV) shows PRV represents a new genus in family Reoviridae and its genome segment S1 sequences group it into two separate sub-genotypes. Virol J 10:230 [View Article][PubMed]
    [Google Scholar]
  13. Kotloff K. L., Nataro J. P., Blackwelder W. C., Nasrin D., Farag T. H., Panchalingam S., Wu Y., Sow S. O., Sur D. et al. 2013; Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the global enteric multicenter study, GEMS): a prospective, case-control study. Lancet 382:209–222 [View Article][PubMed]
    [Google Scholar]
  14. Kumar S., Stecher G., Tamura K. 2016; mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  15. Lupiani B., Subramanian K., Samal S. K. 1995; Aquareoviruses. Annu Rev Fish Dis 5:175–208 [View Article]
    [Google Scholar]
  16. Løvoll M., Alarcón M., Bang Jensen B., Taksdal T., Kristoffersen A. B., Tengs T. 2012; Quantification of piscine reovirus (PRV) at different stages of Atlantic salmon Salmo salar production. Dis Aquat Organ 99:7–12 [View Article][PubMed]
    [Google Scholar]
  17. Marty G. D., Morrison D. B., Bidulka J., Joseph T., Siah A. 2015; Piscine reovirus in wild and farmed salmonids in British Columbia, Canada: 1974–2013. J Fish Dis 38:713–728 [View Article][PubMed]
    [Google Scholar]
  18. Olsen A. B., Hjortaas M., Tengs T., Hellberg H., Johansen R. 2015; First description of a new disease in rainbow trout (Oncorhynchus mykiss (Walbaum)) similar to heart and skeletal muscle inflammation (HSMI) and detection of a gene sequence related to piscine orthoreovirus (PRV). PLoS One 10:e0131638 [View Article][PubMed]
    [Google Scholar]
  19. Palacios G., Lovoll M., Tengs T., Hornig M., Hutchison S., Hui J., Kongtorp R. T., Savji N., Bussetti A. V. et al. 2010; Heart and skeletal muscle inflammation of farmed salmon is associated with infection with a novel reovirus. PLoS One 5:e11487 [View Article][PubMed]
    [Google Scholar]
  20. Philipp D. P., Ridgway M. S. 2002 Black Bass: Ecology, Conservation, and Management Bethesda, MD: American Fisheries Society;
    [Google Scholar]
  21. Picelli S., Faridani O. R., Björklund A. K., Winberg G., Sagasser S., Sandberg R. 2014; Full-length RNA-seq from single cells using smart-seq2. Nat Protoc 9:171–181 [View Article][PubMed]
    [Google Scholar]
  22. Pinto F. L., Lindblad P. 2010; A guide for in-house design of template-switch-based 5′ rapid amplification of cDNA ends systems. Anal Biochem 397:227–232 [View Article][PubMed]
    [Google Scholar]
  23. Seng E. K., Fang Q., Chang S. F., Ngoh G. H., Qin Q. W., Lam T. J., Sin Y. M. 2002; Characterisation of a pathogenic virus isolated from marine threadfin fish (Eleutheronema tetradactylus) during a disease outbreak. Aquaculture 214:1–18 [View Article]
    [Google Scholar]
  24. Siah A., Morrison D. B., Fringuelli E., Savage P., Richmond Z., Johns R., Purcell M. K., Johnson S. C., Saksida S. M. 2015; Piscine reovirus: genomic and molecular phylogenetic analysis from farmed and wild salmonids collected on the Canada/US pacific coast. PLoS One 10:e0141475 [View Article][PubMed]
    [Google Scholar]
  25. Talavera G., Castresana J. 2007; Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst Biol 56:564–577 [View Article][PubMed]
    [Google Scholar]
  26. USFWS AFS-FHS (US Fish and Wildlife Service and American Fisheries Society-Fish Health Section) ( 2014; Standard procedures for aquatic animal health inspections. In AFS-FHS. FHS Blue Book: Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens, 2014 edn. http://afs-fhs.org/bluebook/bluebook-index.php
    [Google Scholar]
  27. Yan X. Y., Wang Y., Xiong L. F., Jian J. C., Wu Z. H. 2014; Phylogenetic analysis of newly isolated grass carp reovirus. Springerplus 3:190 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000568
Loading
/content/journal/jgv/10.1099/jgv.0.000568
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error