1887

Abstract

Rev, an important accessory protein of equine infectious anaemia virus (EIAV), induces the nuclear export of incompletely spliced viral mRNAs. Rev is translated from the mRNA through leaky scanning of the CUG. In this study, the function of the Kozak sequence at the beginning of the ORF was investigated. Deletion or attenuation of the Kozak sequence resulted in expression of an N-terminal 11  aa-truncated Rev in addition to WT Rev. Truncated Rev displayed weaker promotion of Gag expression and processing than WT Rev. Furthermore, EIAV rescued from an infectious molecular clone (pEIAVUK3) with Kozak attenuation exhibited decreased viral replication in host cells . These results provide a new understanding of the relationship between EIAV Rev expression and viral replication.

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2016-09-01
2019-10-15
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