RT Journal Article SR Electronic(1) A1 Swett-Tapia, Cindy A1 Bogaert, Lies A1 de Jong, Pascal A1 van Hoek, Vladimir A1 Schouten, Theo A1 Damen, Irma A1 Spek, Dirk A1 Wanningen, Patrick A1 Radošević, Katarina A1 Widjojoatmodjo, Myra N. A1 Zahn, Roland A1 Custers, Jerome A1 Roy, SoumitraYR 2016 T1 Recombinant measles virus incorporating heterologous viral membrane proteins for use as vaccines JF Journal of General Virology, VO 97 IS 9 SP 2117 OP 2128 DO https://doi.org/10.1099/jgv.0.000523 PB Microbiology Society, SN 1465-2099, AB Recombinant measles virus (rMV) vectors expressing heterologous viral membrane protein antigens are potentially useful as vaccines. Genes encoding the mumps virus haemagglutinin-neuraminidase (MuV-HN), the influenza virus haemagglutinin (Flu-HA) or the respiratory syncytial virus fusion (RSV-F) proteins were inserted into the genome of a live attenuated vaccine strain of measles virus. Additionally, in this case rMV with the MuV-HN or the influenza HA inserts, chimeric constructs were created that harboured the measles virus native haemagglutinin or fusion protein cytoplasmic domains. In all three cases, sucrose-gradient purified preparations of rMV were found to have incorporated the heterologous viral membrane protein on the viral membrane. The possible utility of rMV expressing RSV-F (rMV.RSV-F) as a vaccine was tested in a cotton rat challenge model. Vaccination with rMV.RSV-F efficiently induced neutralizing antibodies against RSV and protected animals from infection with RSV in the lungs., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000523