@article{mbs:/content/journal/jgv/10.1099/jgv.0.000523, author = "Swett-Tapia, Cindy and Bogaert, Lies and de Jong, Pascal and van Hoek, Vladimir and Schouten, Theo and Damen, Irma and Spek, Dirk and Wanningen, Patrick and Radošević, Katarina and Widjojoatmodjo, Myra N. and Zahn, Roland and Custers, Jerome and Roy, Soumitra", title = "Recombinant measles virus incorporating heterologous viral membrane proteins for use as vaccines", journal= "Journal of General Virology", year = "2016", volume = "97", number = "9", pages = "2117-2128", doi = "https://doi.org/10.1099/jgv.0.000523", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000523", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "hemagglutinin", keywords = "respiratory syncitial virus", keywords = "mumps", keywords = "influenza", keywords = "recombinant measles", abstract = "Recombinant measles virus (rMV) vectors expressing heterologous viral membrane protein antigens are potentially useful as vaccines. Genes encoding the mumps virus haemagglutinin-neuraminidase (MuV-HN), the influenza virus haemagglutinin (Flu-HA) or the respiratory syncytial virus fusion (RSV-F) proteins were inserted into the genome of a live attenuated vaccine strain of measles virus. Additionally, in this case rMV with the MuV-HN or the influenza HA inserts, chimeric constructs were created that harboured the measles virus native haemagglutinin or fusion protein cytoplasmic domains. In all three cases, sucrose-gradient purified preparations of rMV were found to have incorporated the heterologous viral membrane protein on the viral membrane. The possible utility of rMV expressing RSV-F (rMV.RSV-F) as a vaccine was tested in a cotton rat challenge model. Vaccination with rMV.RSV-F efficiently induced neutralizing antibodies against RSV and protected animals from infection with RSV in the lungs.", }