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Volume 97, Issue 9

Attenuation and protection efficacy of ORF C gene-deleted recombinant of infectious laryngotracheitis virus Free

Abstract

Infectious laryngotracheitis (ILT) is a highly contagious respiratory disease of chickens caused by infectious laryngotracheitis virus (ILTV). The disease is controlled by the use of live-attenuated vaccines. Previously we reported the complete nucleotide sequence of the ILTV vaccine strain (TCO) and identified a nonsense mutation in the gene encoding the ORF C protein. This suggested that the ORF C protein might be associated with viral virulence. To investigate this, an ILTV recombinant with a deletion in the gene encoding ORF C was constructed using the genome of the virulent United States Department of Agriculture (USDA) challenge strain (USDAch). Compared to the parental virus, the ΔORF C recombinant replicated in chicken kidney (CK) cells with similar kinetics and generated similar titres. This demonstrated that the ORF C deletion had no deleterious effects on replication efficacy . In chickens, the recombinant induced only minor microscopic tracheal lesions when inoculated via the intra-tracheal/ocular route, while the parental strain induced moderate to severe microscopic tracheal lesions, even though virus load in the tracheas were comparable. Groups of chickens vaccinated via eye-drop with the ∆ORFC-ILTV were protected to levels comparable to those elicited by TCO vaccination. To our knowledge, this is the first report that demonstrates the suitability of ∆ORFC as a live-attenuated vaccine to prevent the losses caused by ILTV.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Attenuation , Infectious laryngotracheitis virus (ILTV) and Open reading frame (ORF C)
© Microbiology Society
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2016-09-01
2024-04-23
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Attenuation and protection efficacy of ORF C gene-deleted recombinant of infectious laryngotracheitis virus
J. Gen. Virol. 97, 2352 (2016); https://doi.org/10.1099/jgv.0.000521
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