RT Journal Article SR Electronic(1) A1 Grant, Clare F. J. A1 Carr, B. Veronica A1 Singanallur, Nagendrakumar B. A1 Morris, Jacqueline A1 Gubbins, Simon A1 Hudelet, Pascal A1 Ilott, Martin A1 Charreyre, Catherine A1 Vosloo, Wilna A1 Charleston, BryanYR 2016 T1 The B-cell response to foot-and-mouth-disease virus in cattle following vaccination and live-virus challenge JF Journal of General Virology, VO 97 IS 9 SP 2201 OP 2209 DO https://doi.org/10.1099/jgv.0.000517 PB Microbiology Society, SN 1465-2099, AB Antibodies play a pivotal role against viral infection, and maintenance of protection is dependent on plasma and memory B-cells. Understanding antigen-specific B-cell responses in cattle is essential to inform future vaccine design. We have previously defined T-cell-dependent and -independent B-cell responses in cattle, as a prelude to investigating foot-and-mouth-disease-virus (FMDV)-specific B-cell responses. In this study, we have used an FMDV O-serotype vaccination (O1-Manisa or O SKR) and live-virus challenge (FMDV O SKR) to investigate the homologous and heterologous B-cell response in cattle following both vaccination and live-virus challenge. The FMDV O-serotype vaccines were able to induce a cross-reactive plasma-cell response, specific for both O1-Manisa and O SKR, post-vaccination. Post-FMDV O SKR live-virus challenge, the heterologous O1-Manisa vaccination provided cross-protection against O SKR challenge and cross-reactive O SKR-specific plasma cells were induced. However, vaccination and live-virus challenge were not able to induce a detectable FMDV O-serotype-specific memory B-cell response in any of the cattle. The aim of new FMDV vaccines should be to induce memory responses and increased duration of immunity in cattle., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000517