%0 Journal Article %A Grant, Clare F. J. %A Carr, B. Veronica %A Singanallur, Nagendrakumar B. %A Morris, Jacqueline %A Gubbins, Simon %A Hudelet, Pascal %A Ilott, Martin %A Charreyre, Catherine %A Vosloo, Wilna %A Charleston, Bryan %T The B-cell response to foot-and-mouth-disease virus in cattle following vaccination and live-virus challenge %D 2016 %J Journal of General Virology, %V 97 %N 9 %P 2201-2209 %@ 1465-2099 %R https://doi.org/10.1099/jgv.0.000517 %K B cell %K T cell %K FMDV %K viral immunology %K T-independent %I Microbiology Society, %X Antibodies play a pivotal role against viral infection, and maintenance of protection is dependent on plasma and memory B-cells. Understanding antigen-specific B-cell responses in cattle is essential to inform future vaccine design. We have previously defined T-cell-dependent and -independent B-cell responses in cattle, as a prelude to investigating foot-and-mouth-disease-virus (FMDV)-specific B-cell responses. In this study, we have used an FMDV O-serotype vaccination (O1-Manisa or O SKR) and live-virus challenge (FMDV O SKR) to investigate the homologous and heterologous B-cell response in cattle following both vaccination and live-virus challenge. The FMDV O-serotype vaccines were able to induce a cross-reactive plasma-cell response, specific for both O1-Manisa and O SKR, post-vaccination. Post-FMDV O SKR live-virus challenge, the heterologous O1-Manisa vaccination provided cross-protection against O SKR challenge and cross-reactive O SKR-specific plasma cells were induced. However, vaccination and live-virus challenge were not able to induce a detectable FMDV O-serotype-specific memory B-cell response in any of the cattle. The aim of new FMDV vaccines should be to induce memory responses and increased duration of immunity in cattle. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000517