RT Journal Article SR Electronic(1) A1 Suzuki, Tohru A1 Shibahara, Tomoyuki A1 Yamaguchi, Ryosaku A1 Nakade, Keisuke A1 Yamamoto, Takehisa A1 Miyazaki, Ayako A1 Ohashi, SeiichiYR 2016 T1 Pig epidemic diarrhoea virus S gene variant with a large deletion non-lethal to colostrum-deprived newborn piglets JF Journal of General Virology, VO 97 IS 8 SP 1823 OP 1828 DO https://doi.org/10.1099/jgv.0.000513 PB Microbiology Society, SN 1465-2099, AB We previously identified a third porcine epidemic diarrhoea virus (PEDV) S variant with a large deletion of 582 nucleotides in the 5′ terminal region of the S gene, in addition to the North American type and the S INDELs type. To investigate the pathogenicity of this variant, TTR-2/JPN/2014, we performed experimental infection using colostrum-deprived piglets and compared the results with those from the North American type PEDV, OKN-1/JPN/2013. Fifteen newborn piglets were divided into two groups of 7–8 piglets each and inoculated orally with one of PEDV isolates maintained at the eighth passage in Vero cell culture. Although all PEDV-inoculated piglets showed acute watery diarrhoea, lethality clearly differed between both PEDV-inoculated groups. Moreover, there were differences in virus distribution and lesions on the intestines between the two PEDV-inoculated groups. Therefore, our data suggest that the OKN-1/JPN/2013 PEDV isolate is virulent, whereas the TTR-2/JPN/2014 PEDV isolate is avirulent., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000513