%0 Journal Article %A Robertson, Stacy %A Parker, Alan L. %A Clarke, Carolyn %A Duffy, Margaret R. %A Alba, Raul %A Nicklin, Stuart A. %A Baker, Andrew H. %T Retargeting FX-binding-ablated HAdV-5 to vascular cells by inclusion of the RGD-4C peptide in hexon hypervariable region 7 and the HI loop %D 2016 %J Journal of General Virology, %V 97 %N 8 %P 1911-1916 %@ 1465-2099 %R https://doi.org/10.1099/jgv.0.000505 %K targeting %K coagulation factor X %K fibre %K hexon %K adenovirus %K gene therapy %I Microbiology Society, %X Recent studies have generated interest in the function of human adenovirus serotype 5 (HAdV-5) hexon:  factor X (FX) binding and subsequent hepatocyte transduction and interaction with the immune system. Here, we retargeted adenovirus serotype 5 vectors, ablated for FX interaction, by replacing amino acids in hexon HVR7 with RGD-4C or inserting the peptide into the fibre HI loop. These genetic modifications in the capsid were compatible with virus assembly, and could efficiently retarget transduction of the vector via the αvβ3/5 integrin-mediated pathway, but did not alter immune recognition by pre-existing human neutralizing anti-HAdV-5 antibodies or by natural antibodies in mouse serum. Thus, FX-binding-ablated HAdV-5 can be retargeted but remain sensitive to immune-mediated attack. These findings further refine HAdV-5-based vectors for human gene therapy and inform future vector development. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000505