1887

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Volume 97, Issue 8

Exposure of rhesus monkeys to cowpox virus Brighton Red by large-particle aerosol droplets results in an upper respiratory tract disease Free

Abstract

We previously demonstrated that small-particle (0.5–3.0 µm) aerosol infection of rhesus monkeys () with cowpox virus (CPXV)-Brighton Red (BR) results in fulminant respiratory tract disease characterized by severe lung parenchymal pathology but only limited systemic virus dissemination and limited classic epidermal pox-like lesion development ( ). Based on these results, and to further develop CPXV as an improved model of human smallpox, we evaluated a novel large-particle aerosol (7.0–9.0 µm) exposure of rhesus monkeys to CPXV-BR and monitored for respiratory tract disease by serial computed tomography (CT). As expected, the upper respiratory tract and large airways were the major sites of virus-induced pathology following large-particle aerosol exposure. Large-particle aerosol CPXV exposure of rhesus macaques resulted in severe upper airway and large airway pathology with limited systemic dissemination.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): aerosol , large-particle aerosol , nonhuman primate model , orthopoxvirus , smallpox and variola virus
2016
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2016-08-01
2022-01-22
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Exposure of rhesus monkeys to cowpox virus Brighton Red by large-particle aerosol droplets results in an upper respiratory tract disease
J. Gen. Virol. 97, 1942 (2016); https://doi.org/10.1099/jgv.0.000501
/content/journal/jgv/10.1099/jgv.0.000501
/content/journal/jgv/10.1099/jgv.0.000501
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