1887

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Volume 97, Issue 8

Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment Free

Abstract

Group C rotaviruses (RVC) are enteric pathogens of humans and animals. Whole-genome sequences are available only for few RVCs, leaving gaps in our knowledge about their genetic diversity. We determined the full-length genome sequence of two human RVCs (PR2593/2004 and PR713/2012), detected in Italy from hospital-based surveillance for rotavirus infection in 2004 and 2012. In the 11 RNA genomic segments, the two Italian RVCs segregated within separate intra-genotypic lineages showed variation ranging from 1.9 % (VP6) to 15.9 % (VP3) at the nucleotide level. Comprehensive analysis of human RVC sequences available in the databases allowed us to reveal the existence of at least two major genome configurations, defined as type I and type II. Human RVCs of type I were all associated with the M3 VP3 genotype, including the Italian strain PR2593/2004. Conversely, human RVCs of type II were all associated with the M2 VP3 genotype, including the Italian strain PR713/2012. Reassortant RVC strains between these major genome configurations were identified. Although only a few full-genome sequences of human RVCs, mostly of Asian origin, are available, the analysis of human RVC sequences retrieved from the databases indicates that at least two intra-genotypic RVC lineages circulate in European countries. Gathering more sequence data is necessary to develop a standardized genotype and intra-genotypic lineage classification system useful for epidemiological investigations and avoiding confusion in the literature.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Full-Genome , Gastroenteritis , Genetic diversity , Group C Rotavirus and Phylogenetic analysis
© 2016 The Authors
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2016-08-01
2024-04-19
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Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment
J. Gen. Virol. 97, 1888 (2016); https://doi.org/10.1099/jgv.0.000497
/content/journal/jgv/10.1099/jgv.0.000497
/content/journal/jgv/10.1099/jgv.0.000497
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