1887

Journal of General Virology header logo

Volume 97, Issue 7

Application of the thermofluor PaSTRy technique for improving foot-and-mouth disease virus vaccine formulation Open Access

Abstract

Foot-and-mouth disease (FMD) has a major economic impact throughout the world and is a considerable threat to food security. Current FMD virus (FMDV) vaccines are made from chemically inactivated virus and need to contain intact viral capsids to maximize efficacy. FMDV exists as seven serotypes, each made up by a number of constantly evolving subtypes. A lack of immunological cross-reactivity between serotypes and between some strains within a serotype greatly complicates efforts to control FMD by vaccination. Thus, vaccines for one serotype do not afford protection against the others, and multiple-serotype-specific vaccines are required for effective control. The FMDV serotypes exhibit variation in their thermostability, and the capsids of inactivated preparations of the O, C and SAT serotypes are particularly susceptible to dissociation at elevated temperature. Methods to quantify capsid stability are currently limited, lack sensitivity and cannot accurately reflect differences in thermostability. Thus, new, more sensitive approaches to quantify capsid stability would be of great value for the production of more stable vaccines and to assess the effect of production conditions on vaccine preparations. Here we have investigated the application of a novel methodology (termed PaSTRy) that utilizes an RNA-binding fluorescent dye and a quantitative (q)PCR machine to monitor viral genome release and hence dissociation of the FMDV capsid during a slow incremental increase in temperature. PaSTRy was used to characterize capsid stability of all FMDV serotypes. Furthermore, we have used this approach to identify stabilizing factors for the most labile FMDV serotypes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): capsid stability , FMDV , PaSTRy and vaccine production
© 2016 The Authors
Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000462
2016-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/97/7/1557.html?itemId=/content/journal/jgv/10.1099/jgv.0.000462&mimeType=html&fmt=ahah

References

  1. Adebayo A. A., Sim-Brandenburg J. W., Emmel H., Olaleye D. O., Niedrig M. 1998; Stability of 17D yellow fever virus vaccine using different stabilizers. Biologicals 26:309–316 [View Article][PubMed]
     [Google Scholar]
  2. Brehm K. E., Ferris N. P., Lenk M., Riebe R., Haas B. 2009; Highly sensitive fetal goat tongue cell line for detection and isolation of foot-and-mouth disease virus. J Clin Microbiol 47:3156–3160 [View Article][PubMed]
     [Google Scholar]
  3. Dar P., Kalaivanan R., Sied N., Mamo B., Kishore S., Suryanarayana V. V., Kondabattula G. 2013; Montanide ISA 201 adjuvanted FMD vaccine induces improved immune responses and protection in cattle. Vaccine 31:3327–3332 [View Article][PubMed]
     [Google Scholar]
  4. Doel T. R., Baccarini P. J. 1981; Thermal stability of foot-and-mouth disease virus. Arch Virol 70:21–32[PubMed] [CrossRef]
     [Google Scholar]
  5. Ericsson U. B., Hallberg B. M., Detitta G. T., Dekker N., Nordlund P. 2006; Thermofluor-based high-throughput stability optimization of proteins for structural studies. Anal Biochem 357:289–298 [View Article][PubMed]
     [Google Scholar]
  6. Grosz D. D., van Geelen A., Gallup J. M., Hostetter S. J., Derscheid R. J., Ackermann M. R. 2014; Sucrose stabilization of Respiratory Syncytial Virus (RSV) during nebulization and experimental infection. BMC Res Notes 7:158 [View Article][PubMed]
     [Google Scholar]
  7. Jackson T., Sheppard D., Denyer M., Blakemore W., King A. M. 2000; The epithelial integrin alphavbeta6 is a receptor for foot-and-mouth disease virus. J Virol 74:4949–4956[PubMed] [CrossRef]
     [Google Scholar]
  8. Kotecha A., Seago J., Scott K., Burman A., Loureiro S., Ren J., Porta C., Ginn H. M., Jackson T. et al. 2015; Structure-based energetics of protein interfaces guides foot-and-mouth disease virus vaccine design. Nat Struct Mol Biol 22:788–794 [View Article][PubMed]
     [Google Scholar]
  9. Mezzasalma T. M., Kranz J. K., Chan W., Struble G. T., Schalk-Hihi C., Deckman I. C., Springer B. A., Todd M. J. 2007; Enhancing recombinant protein quality and yield by protein stability profiling. J Biomol Screen 12:418–428 [View Article][PubMed]
     [Google Scholar]
  10. Mirchamsy H., Shafyi A., Mahinpour M., Nazari P. 1978; Stabilizing effect of magnesium chloride and sucrose on Sabin live polio vaccine. Dev Biol Stand 41:255–257[PubMed]
     [Google Scholar]
  11. Niesen F. H., Berglund H., Vedadi M. 2007; The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability. Nat Protoc 2:2212–2221 [View Article][PubMed]
     [Google Scholar]
  12. Riyesh T., Balamurugan V., Sen A., Bhanuprakash V., Venkatesan G., Yadav V., Singh R. K. 2011; Evaluation of efficacy of stabilizers on the thermostability of live attenuated thermo-adapted Peste des petits ruminants vaccines. Virol Sin 26:324–337 [View Article][PubMed]
     [Google Scholar]
  13. Rombaut B., Verheyden B., Andries K., Boeyé A. 1994; Thermal inactivation of oral polio vaccine: contribution of RNA and protein inactivation. J Virol 68:6454–6457[PubMed]
     [Google Scholar]
  14. Senisterra G. A., Finerty P. J. 2009; High throughput methods of assessing protein stability and aggregation. Mol Biosyst 5:217–223 [View Article][PubMed]
     [Google Scholar]
  15. Srivastava A. K. 1989; Stabilization of the attenuated poliovirus type 3 vaccine strain by sucrose. Acta Virol 33:188–190[PubMed]
     [Google Scholar]
  16. Terry G. M., Syred A., Rweyemamu M. M. 1983; Studies on the stability of foot-and-mouth disease virus using absorbance – temperature profiles. Dev Biol Stand 55:117–120[PubMed]
     [Google Scholar]
  17. Vedadi M., Niesen F. H., Allali-Hassani A., Fedorov O. Y., Finerty P. J., Wasney G. A., Yeung R., Arrowsmith C., Ball L. J. et al. 2006; Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination. Proc Natl Acad Sci U S A 103:15835–15840 [View Article][PubMed]
     [Google Scholar]
  18. Walter T. S., Ren J., Tuthill T. J., Rowlands D. J., Stuart D. I., Fry E. E. 2012; A plate-based high-throughput assay for virus stability and vaccine formulation. J Virol Methods 185:166–170 [View Article][PubMed]
     [Google Scholar]
  19. Wiggan O., Livengood J. A., Silengo S. J., Kinney R. M., Osorio J. E., Huang C. Y., Stinchcomb D. T. 2011; Novel formulations enhance the thermal stability of live-attenuated flavivirus vaccines. Vaccine 29:7456–7462 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000462
Loading
Application of the thermofluor PaSTRy technique for improving foot-and-mouth disease virus vaccine formulation
J. Gen. Virol. 97, 1557 (2016); https://doi.org/10.1099/jgv.0.000462
/content/journal/jgv/10.1099/jgv.0.000462
/content/journal/jgv/10.1099/jgv.0.000462
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error