1887

Abstract

Cucurbit chlorotic yellows virus (CCYV), a recently identified bipartite crinivirus, causes economic losses in cucurbit plants. CCYV is naturally transmitted only by whitefly . Here we constructed full-length cDNA clones of CCYV (RNA1 and RNA2) fused to the T7 RNA polymerase promoter and the cauliflower mosaic virus 35S promoter. CCYV replicated and accumulated efficiently in protoplasts transfected with transcripts. Without RNA2, RNA1 replicated efficiently in protoplasts. Agroinoculation with the infectious cDNA clones of CCYV resulted in systemic infection in the host plants of and. Virus derived from the infectious clones could be transmitted between cucumber plants by vector whiteflies. This system will greatly enhance the reverse genetic studies of CCYV gene functions.

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2016-06-01
2020-01-28
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