RT Journal Article SR Electronic(1) A1 Suzuki, Ryosuke A1 Saito, Kenji A1 Matsuda, Mami A1 Sato, Mitsuru A1 Kanegae, Yumi A1 Shi, Guoli A1 Watashi, Koichi A1 Aizaki, Hideki A1 Chiba, Joe A1 Saito, Izumu A1 Wakita, Takaji A1 Suzuki, TetsuroYR 2016 T1 Single-domain intrabodies against hepatitis C virus core inhibit viral propagation and core-induced NFκB activation JF Journal of General Virology, VO 97 IS 4 SP 887 OP 892 DO https://doi.org/10.1099/jgv.0.000423 PB Microbiology Society, SN 1465-2099, AB Hepatitis C virus (HCV) core plays a key role in viral particle formation and is involved in viral pathogenesis. Here, constructs for single-domain intrabodies consisting of variable regions derived from mouse mAbs against HCV core were established. Expressed single-domain intrabodies were shown to bind to HCV core, and inhibit the growth of cell culture-produced HCV derived from JFH-1 (genotype 2a) and a TH (genotype 1b)/JFH-1 chimera. Adenovirus vectors expressing intrabodies were also capable of reducing HCV propagation. Intrabody expression did not affect viral entry or genome replication of single-round infectious trans-complemented HCV particles. However, intrabody expression reduced intracellular and extracellular infectious titres in CD81-defective Huh7-25 cells transfected with the HCV genome, suggesting that these intrabodies impair HCV assembly. Furthermore, intrabody expression suppressed HCV core-induced NFκB promoter activity. These intrabodies may therefore serve as tools for elucidating the role of core in HCV pathogenesis., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000423