@article{mbs:/content/journal/jgv/10.1099/jgv.0.000423, author = "Suzuki, Ryosuke and Saito, Kenji and Matsuda, Mami and Sato, Mitsuru and Kanegae, Yumi and Shi, Guoli and Watashi, Koichi and Aizaki, Hideki and Chiba, Joe and Saito, Izumu and Wakita, Takaji and Suzuki, Tetsuro", title = "Single-domain intrabodies against hepatitis C virus core inhibit viral propagation and core-induced NFκB activation", journal= "Journal of General Virology", year = "2016", volume = "97", number = "4", pages = "887-892", doi = "https://doi.org/10.1099/jgv.0.000423", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000423", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Hepatitis C virus (HCV) core plays a key role in viral particle formation and is involved in viral pathogenesis. Here, constructs for single-domain intrabodies consisting of variable regions derived from mouse mAbs against HCV core were established. Expressed single-domain intrabodies were shown to bind to HCV core, and inhibit the growth of cell culture-produced HCV derived from JFH-1 (genotype 2a) and a TH (genotype 1b)/JFH-1 chimera. Adenovirus vectors expressing intrabodies were also capable of reducing HCV propagation. Intrabody expression did not affect viral entry or genome replication of single-round infectious trans-complemented HCV particles. However, intrabody expression reduced intracellular and extracellular infectious titres in CD81-defective Huh7-25 cells transfected with the HCV genome, suggesting that these intrabodies impair HCV assembly. Furthermore, intrabody expression suppressed HCV core-induced NFκB promoter activity. These intrabodies may therefore serve as tools for elucidating the role of core in HCV pathogenesis.", }