@article{mbs:/content/journal/jgv/10.1099/jgv.0.000420, author = "Wang, Yixin and Li, Jianliang and Li, Yang and Fang, Lichun and Sun, Xiaolong and Chang, Shuang and Zhao, Peng and Cui, Zhizhong", title = "Identification of avian leukosis virus subgroup J-associated acutely transforming viruses carrying the v-src oncogene in layer chickens", journal= "Journal of General Virology", year = "2016", volume = "97", number = "5", pages = "1240-1248", doi = "https://doi.org/10.1099/jgv.0.000420", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000420", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "To elucidate the molecular basis for the rapid oncogenicity of an acutely transforming avian leukosis virus (ALV), isolated from fibrosarcomas in Hy-Line Brown commercial layer chickens infected with ALV subgroup J (ALV-J), the complete genomic structure of the provirus was determined. In addition to ALV-J replication-complete virus SDAU1102, five proviral DNA genomes, named SJ-1, SJ-2, SJ-3, SJ-4 and SJ-5, carrying different lengths of the v-src oncogene were amplified from original tumours and chicken embryo fibroblasts (CEFs) infected with viral stocks. The genomic sequences of the SJ-1–SJ-5 provirus were closely related to that of SDAU1102 but were defective. The results of Western blot analysis and immunohistochemical staining also showed overexpression of the p60v-src protein in infected CEFs and tumour tissue. To the best of our knowledge, this is the first report of the isolation and identification of acutely transforming viruses carrying the v-src oncogene with ALV-J as the helper virus. It also offers insight into the generation of acutely transforming ALVs carrying the v-src oncogene.", }