1887

Abstract

At least two genetically different porcine epidemic diarrhoea virus (PEDV) strains have been identified in the USA: US PEDV prototype and S-INDEL-variant strains. The objective of this study was to compare the pathogenicity differences of the US PEDV prototype and S-INDEL-variant strains in conventional neonatal piglets under experimental infections. Fifty PEDV-negative 5-day-old pigs were divided into five groups of ten pigs each and were inoculated orogastrically with three US PEDV prototype isolates (IN19338/2013, NC35140/2013 and NC49469/2013), an S-INDEL-variant isolate (IL20697/2014), and virus-negative culture medium, respectively, with virus titres of 10 TCID ml, 10 ml per pig. All three PEDV prototype isolates tested in this study, regardless of their phylogenetic clades, had similar pathogenicity and caused severe enteric disease in 5-day-old pigs as evidenced by clinical signs, faecal virus shedding, and gross and histopathological lesions. Compared with pigs inoculated with the three US PEDV prototype isolates, pigs inoculated with the S-INDEL-variant isolate had significantly diminished clinical signs, virus shedding in faeces, gross lesions in small intestines, caeca and colons, histopathological lesions in small intestines, and immunohistochemistry staining in ileum. However, the US PEDV prototype and the S-INDEL-variant strains induced similar viraemia levels in inoculated pigs. Whole genome sequences of the PEDV prototype and S-INDEL-variant strains were determined, but the molecular basis of virulence differences between these PEDV strains remains to be elucidated using a reverse genetics approach.

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2016-05-01
2020-01-22
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References

  1. Boniotti M. B., Papetti A., Lavazza A., Alborali G., Sozzi E., Chiapponi C., Faccini S., Bonilauri P., Cordioli P., Marthaler D.. 2016; Porcine epidemic diarrhea virus and discovery of a recombinant swine enteric coronavirus, Italy. Emerg Infect Dis22:83–87 [CrossRef][PubMed]
    [Google Scholar]
  2. Chen Q., Li G., Stasko J., Thomas J. T., Stensland W. R., Pillatzki A. E., Gauger P. C., Schwartz K. J., Madson D., other authors. 2014; Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States. J Clin Microbiol52:234–243 [CrossRef][PubMed]
    [Google Scholar]
  3. Chen Q., Gauger P., Stafne M., Thomas J., Arruda P., Burrough E., Madson D., Brodie J., Magstadt D., other authors. 2015; Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets. Virology482:51–59 [CrossRef][PubMed]
    [Google Scholar]
  4. Cima G.. 2014; PED virus reinfecting U.S. herds. Virus estimated to have killed 7 million-plus pigs. J Am Vet Med Assoc245:166–167[PubMed]
    [Google Scholar]
  5. Dastjerdi A., Carr J., Ellis R. J., Steinbach F., Williamson S.. 2015; Porcine epidemic diarrhea virus among farmed pigs, Ukraine. Emerg Infect Dis21:2235–2237 [CrossRef][PubMed]
    [Google Scholar]
  6. Goede D., Murtaugh M. P., Nerem J., Yeske P., Rossow K., Morrison R.. 2015; Previous infection of sows with a mild strain of porcine epidemic diarrhea virus confers protection against infection with a severe strain. Vet Microbiol176:161–164 [CrossRef][PubMed]
    [Google Scholar]
  7. Grasland B., Bigault L., Bernard C., Quenault H., Toulouse O., Fablet C., Rose N., Touzain F., Blanchard Y.. 2015; Complete genome sequence of a porcine epidemic diarrhea s gene indel strain isolated in France in December 2014. Genome Announc3:e00535-15 [CrossRef][PubMed]
    [Google Scholar]
  8. Hall T. A.. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser41:95–98
    [Google Scholar]
  9. Hanke D., Jenckel M., Petrov A., Ritzmann M., Stadler J., Akimkin V., Blome S., Pohlmann A., Schirrmeier H., other authors. 2015; Comparison of porcine epidemic diarrhea viruses from Germany and the United States, 2014. Emerg Infect Dis21:493–496 [CrossRef][PubMed]
    [Google Scholar]
  10. Huang Y. W., Dickerman A. W., Piñeyro P., Li L., Fang L., Kiehne R., Opriessnig T., Meng X. J.. 2013; Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States. MBio4:e00737-13 [CrossRef][PubMed]
    [Google Scholar]
  11. Jung K., Wang Q., Scheuer K. A., Lu Z., Zhang Y., Saif L. J.. 2014; Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs. Emerg Infect Dis20:662–665 [CrossRef][PubMed]
    [Google Scholar]
  12. Jung K., Annamalai T., Lu Z., Saif L. J.. 2015a; Comparative pathogenesis of US porcine epidemic diarrhea virus (PEDV) strain PC21A in conventional 9-day-old nursing piglets vs. 26-day-old weaned pigs. Vet Microbiol178:31–40 [CrossRef][PubMed]
    [Google Scholar]
  13. Jung K., Eyerly B., Annamalai T., Lu Z., Saif L. J.. 2015b; Structural alteration of tight and adherens junctions in villous and crypt epithelium of the small and large intestine of conventional nursing piglets infected with porcine epidemic diarrhea virus. Vet Microbiol177:373–378 [CrossRef][PubMed]
    [Google Scholar]
  14. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., other authors. 2007; Clustal W and Clustal_X version 2.0. Bioinformatics23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  15. Lee S., Lee C.. 2014; Outbreak-related porcine epidemic diarrhea virus strains similar to US strains, South Korea, 2013. Emerg Infect Dis20:1223–1226 [CrossRef][PubMed]
    [Google Scholar]
  16. Lee S., Park G. S., Shin J. H., Lee C.. 2014; Full-genome sequence analysis of a variant strain of porcine epidemic diarrhea virus in South Korea. Genome Announc2:e01116-14 [CrossRef][PubMed]
    [Google Scholar]
  17. Li W., Li H., Liu Y., Pan Y., Deng F., Song Y., Tang X., He Q.. 2012; New variants of porcine epidemic diarrhea virus, China, 2011. Emerg Infect Dis18:1350–1353 [CrossRef][PubMed]
    [Google Scholar]
  18. Lin C. N., Chung W. B., Chang S. W., Wen C. C., Liu H., Chien C. H., Chiou M. T.. 2014; US-like strain of porcine epidemic diarrhea virus outbreaks in Taiwan, 2013–2014. J Vet Med Sci76:1297–1299 [CrossRef][PubMed]
    [Google Scholar]
  19. Lin C. M., Annamalai T., Liu X., Gao X., Lu Z., El-Tholoth M., Hu H., Saif L. J., Wang Q.. 2015a; Experimental infection of a US spike-insertion deletion porcine epidemic diarrhea virus in conventional nursing piglets and cross-protection to the original US PEDV infection. Vet Res46:134 [CrossRef][PubMed]
    [Google Scholar]
  20. Lin C. M., Gao X., Oka T., Vlasova A. N., Esseili M. A., Wang Q., Saif L. J.. 2015b; Antigenic relationships among porcine epidemic diarrhea virus and transmissible gastroenteritis virus strains. J Virol89:3332–3342 [CrossRef][PubMed]
    [Google Scholar]
  21. Liu X., Lin C. M., Annamalai T., Gao X., Lu Z., Esseili M. A., Jung K., El-Tholoth M., Saif L. J., Wang Q.. 2015; Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain. Vet Res46:109 [CrossRef][PubMed]
    [Google Scholar]
  22. Lowe J., Gauger P., Harmon K., Zhang J., Connor J., Yeske P., Loula T., Levis I., Dufresne L., Main R.. 2014; Role of transportation in spread of porcine epidemic diarrhea virus infection, United States. Emerg Infect Dis20:872–874 [CrossRef][PubMed]
    [Google Scholar]
  23. Madson D. M., Magstadt D. R., Arruda P. H., Hoang H., Sun D., Bower L. P., Bhandari M., Burrough E. R., Gauger P. C., other authors. 2014; Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs. Vet Microbiol174:60–68 [CrossRef][PubMed]
    [Google Scholar]
  24. Madson D. M., Arruda P. H., Magstadt D. R., Burrough E. R., Hoang H., Sun D., Bower L. P., Bhandari M., Gauger P. C., other authors. 2016; Characterization of porcine epidemic diarrhea virus isolate US/Iowa/18984/2013 infection in 1-day-old Cesarean-derived colostrum-deprived piglets. Vet Pathol53:44–52 [CrossRef][PubMed]
    [Google Scholar]
  25. Mesquita J. R., Hakze-van der Honing R., Almeida A., Lourenço M., van der Poel W. H., Nascimento M. S.. 2015; Outbreak of porcine epidemic diarrhea virus in Portugal, 2015. Transbound Emerg Dis62:586–588 [CrossRef][PubMed]
    [Google Scholar]
  26. Ojkic D., Hazlett M., Fairles J., Marom A., Slavic D., Maxie G., Alexandersen S., Pasick J., Alsop J., Burlatschenko S.. 2015; The first case of porcine epidemic diarrhea in Canada. Can Vet J56:149–152[PubMed]
    [Google Scholar]
  27. Oldham J.. 1972; Epidemic diarrhoea: How it all began. Letter to the editor. Pig farming(Suppl.)72–73
    [Google Scholar]
  28. Saif L. J.. 2011; Coronaviridae. In Fenner's Veterinary Virology, 4th edn. pp393–413Edited by MacLachlan N. J., Dubovi E. J.. ch. 24 Cambridge, Massachusetts, USA.: Elsevier-Academic Press;
    [Google Scholar]
  29. Saif L. J., Pensaert M. B., Sestak K., Yeo S. G., Jung K.. 2012; Coronaviruses. In Diseases of Swine, 10th edn. pp501–524Edited by Zimmerman J. J., Karriker L. A., Ramirez A., Schwartz K. J., Stevenson G. W.. Chichester, West Sussex, UK.: Wiley-Blackwell;
    [Google Scholar]
  30. Song D., Park B.. 2012; Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines. Virus Genes44:167–175 [CrossRef][PubMed]
    [Google Scholar]
  31. Stadler J., Zoels S., Fux R., Hanke D., Pohlmann A., Blome S., Weissenböck H., Weissenbacher-Lang C., Ritzmann M., Ladinig A.. 2015; Emergence of porcine epidemic diarrhea virus in southern Germany. BMC Vet Res11:142 [CrossRef][PubMed]
    [Google Scholar]
  32. Steinrigl A., Revilla Fernández S., Stoiber F., Pikalo J., Sattler T., Schmoll F.. 2015; First detection, clinical presentation and phylogenetic characterization of porcine epidemic diarrhea virus in Austria. BMC Vet Res11:310 [CrossRef][PubMed]
    [Google Scholar]
  33. Stevenson G. W., Hoang H., Schwartz K. J., Burrough E. R., Sun D., Madson D., Cooper V. L., Pillatzki A., Gauger P., other authors. 2013; Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences. J Vet Diagn Invest25:649–654 [CrossRef][PubMed]
    [Google Scholar]
  34. Sun R. Q., Cai R. J., Chen Y. Q., Liang P. S., Chen D. K., Song C. X.. 2012; Outbreak of porcine epidemic diarrhea in suckling piglets, China. Emerg Infect Dis18:161–163 [CrossRef][PubMed]
    [Google Scholar]
  35. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  36. Theuns S., Conceição-Neto N., Christiaens I., Zeller M., Desmarets L. M., Roukaerts I. D., Acar D. D., Heylen E., Matthijnssens J., Nauwynck H. J.. 2015; Complete genome sequence of a porcine epidemic diarrhea virus from a novel outbreak in Belgium, January 2015. Genome Announc3:e00506–e00515 [CrossRef][PubMed]
    [Google Scholar]
  37. Thomas J. T., Chen Q., Gauger P. C., Giménez-Lirola L. G., Sinha A., Harmon K. M., Madson D. M., Burrough E. R., Magstadt D. R., other authors. 2015; Effect of porcine epidemic diarrhea virus infectious doses on infection outcomes in naı¨ve conventional neonatal and weaned pigs. PLoS One10:e0139266 [CrossRef][PubMed]
    [Google Scholar]
  38. Van Diep N., Norimine J., Sueyoshi M., Lan N. T., Hirai T., Yamaguchi R.. 2015; US-like isolates of porcine epidemic diarrhea virus from Japanese outbreaks between 2013 and 2014. SpringerPlus4:756 [CrossRef][PubMed]
    [Google Scholar]
  39. Vlasova A. N., Marthaler D., Wang Q., Culhane M. R., Rossow K. D., Rovira A., Collins J., Saif L. J.. 2014; Distinct characteristics and complex evolution of PEDV strains, North America, May 2013–February 2014. Emerg Infect Dis20:1620–1628 [CrossRef][PubMed]
    [Google Scholar]
  40. Wang L., Byrum B., Zhang Y.. 2014; New variant of porcine epidemic diarrhea virus, United States, 2014. Emerg Infect Dis20:917–919 [CrossRef][PubMed]
    [Google Scholar]
  41. Yamamoto R., Soma J., Nakanishi M., Yamaguchi R., Niinuma S.. 2015; Isolation and experimental inoculation of an S INDEL strain of porcine epidemic diarrhea virus in Japan. Res Vet Sci103:103–106 [CrossRef][PubMed]
    [Google Scholar]
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