1887

Abstract

Porcine circovirus type 2 (PCV2) is the pivotal pathogen causing porcine circovirus-associated diseases. In this study, 62 PCV2 isolates were identified from seven farms in southern China from 2013 to 2015 and phylogenetic trees were reconstructed based on whole-genome sequences or the gene. In this investigation, PCV2b was the main genotype in circulation throughout these farms. Furthermore, an emerging mutant (PCV2b-1C), isolated from PCV2-vaccinated farms, was the predominant strain prevalent on these farms. In addition, we isolated a new cluster that may represent evolution of the virus through recombination of PCV2b-1A/1B and PCV2b-1C. Finally, we discuss evidence that antigenicity and surface structure variation of the capsid resulted from mutation of the C-terminal loop (Loop CT) of the PCV2b-1C Cap .

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2016-04-01
2024-12-05
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References

  1. Alarcon P., Rushton J., Wieland B. 2013; Cost of post-weaning multi-systemic wasting syndrome and porcine circovirus type-2 subclinical infection in England – an economic disease model. Prev Vet Med 110:88–102 [View Article][PubMed]
    [Google Scholar]
  2. An D. J., Roh I. S., Song D. S., Park C. K., Park B. K. 2007; Phylogenetic characterization of porcine circovirus type 2 in PMWS and PDNS Korean pigs between 1999 and 2006. Virus Res 129:115–122 [View Article][PubMed]
    [Google Scholar]
  3. Beach N. M., Meng X. J. 2012; Efficacy and future prospects of commercially available and experimental vaccines against porcine circovirus type 2 (PCV2). Virus Res 164:33–42 [View Article][PubMed]
    [Google Scholar]
  4. Cai L., Ni J., Xia Y., Zi Z., Ning K., Qiu P., Li X., Wang B., Liu Q., other authors. 2012; Identification of an emerging recombinant cluster in porcine circovirus type 2. Virus Res 165:95–102 [View Article][PubMed]
    [Google Scholar]
  5. Chae C. 2005; A review of porcine circovirus 2-associated syndromes and diseases. Vet J 169:326–336 [View Article][PubMed]
    [Google Scholar]
  6. Cheung A. K. 2003; The essential and nonessential transcription units for viral protein synthesis and DNA replication of porcine circovirus type 2. Virology 313:452–459 [View Article][PubMed]
    [Google Scholar]
  7. Cheung A. K. 2006; Rolling-circle replication of an animal circovirus genome in a theta-replicating bacterial plasmid in Escherichia coli . J Virol 80:8686–8694 [View Article][PubMed]
    [Google Scholar]
  8. Cortey M., Olvera A., Grau-Roma L., Segalés J. 2011; Further comments on porcine circovirus type 2 (PCV2) genotype definition and nomenclature. Vet Microbiol 149:522–523 [View Article][PubMed]
    [Google Scholar]
  9. de Castro A. M., Cruz T. F., Salgado V. R., Kanashiro T. M., Ferrari K. L., Araujo J. P. Jr, Brandão P. E., Richtzenhain L. J. 2012; Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil. Acta Vet Scand 54:29 [View Article][PubMed]
    [Google Scholar]
  10. Eddicks M., Fux R., Szikora F., Eddicks L., Majzoub-Altweck M., Hermanns W., Sutter G., Palzer A., Banholzer E., Ritzmann M. 2015; Detection of a new cluster of porcine circovirus type 2b strains in domestic pigs in Germany. Vet Microbiol 176:337–343 [View Article][PubMed]
    [Google Scholar]
  11. Finsterbusch T., Mankertz A. 2009; Porcine circoviruses – small but powerful. Virus Res 143:177–183 [View Article][PubMed]
    [Google Scholar]
  12. Gillespie J., Opriessnig T., Meng X. J., Pelzer K., Buechner-Maxwell V. 2009; Porcine circovirus type 2 and porcine circovirus-associated disease. J Vet Intern Med 23:1151–1163 [View Article][PubMed]
    [Google Scholar]
  13. Grau-Roma L., Crisci E., Sibila M., López-Soria S., Nofrarias M., Cortey M., Fraile L., Olvera A., Segalés J. 2008; A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence. Vet Microbiol 128:23–35 [View Article][PubMed]
    [Google Scholar]
  14. Guo L., Fu Y., Wang Y., Lu Y., Wei Y., Tang Q., Fan P., Liu J., Zhang L., other authors. 2012; A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in capsid protein showed more virulence in vivo, compared with classical PCV2a/b strain. PLoS One 7:e41463 [View Article][PubMed]
    [Google Scholar]
  15. Huang L. P., Lu Y. H., Wei Y. W., Guo L. J., Liu C. M. 2011; Identification of one critical amino acid that determines a conformational neutralizing epitope in the capsid protein of porcine circovirus type 2. BMC Microbiol 11:188 [View Article][PubMed]
    [Google Scholar]
  16. Juhan N. M., LeRoith T., Opriessnig T., Meng X. J. 2010; The open reading frame 3 (ORF3) of porcine circovirus type 2 (PCV2) is dispensable for virus infection but evidence of reduced pathogenicity is limited in pigs infected by an ORF3-null PCV2 mutant. Virus Res 147:60–66 [View Article][PubMed]
    [Google Scholar]
  17. Khayat R., Brunn N., Speir J. A., Hardham J. M., Ankenbauer R. G., Schneemann A., Johnson J. E. 2011; The 2.3-angstrom structure of porcine circovirus 2. J Virol 85:7856–7862 [View Article][PubMed]
    [Google Scholar]
  18. Lekcharoensuk P., Morozov I., Paul P. S., Thangthumniyom N., Wajjawalku W., Meng X. J. 2004; Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2. J Virol 78:8135–8145 [View Article][PubMed]
    [Google Scholar]
  19. Liu J., Chen I., Kwang J. 2005; Characterization of a previously unidentified viral protein in porcine circovirus type 2-infected cells and its role in virus-induced apoptosis. J Virol 79:8262–8274 [View Article][PubMed]
    [Google Scholar]
  20. Liu J., Huang L., Wei Y., Tang Q., Liu D., Wang Y., Li S., Guo L., Wu H., Liu C. 2013; Amino acid mutations in the capsid protein produce novel porcine circovirus type 2 neutralizing epitopes. Vet Microbiol 165:260–267 [View Article][PubMed]
    [Google Scholar]
  21. Ma C. M., Hon C. C., Lam T. Y., Li V. Y., Wong C. K., de Oliveira T., Leung F. C. 2007; Evidence for recombination in natural populations of porcine circovirus type 2 in Hong Kong and mainland China. J Gen Virol 88:1733–1737 [View Article][PubMed]
    [Google Scholar]
  22. Manokaran G., Lin Y. N., Soh M. L., Lim E. A., Lim C. W., Tan B. H. 2008; Detection of porcine circovirus type 2 in pigs imported from Indonesia. Vet Microbiol 132:165–170 [View Article][PubMed]
    [Google Scholar]
  23. Misinzo G., Delputte P. L., Meerts P., Lefebvre D. J., Nauwynck H. J. 2006; Porcine circovirus 2 uses heparan sulfate and chondroitin sulfate B glycosaminoglycans as receptors for its attachment to host cells. J Virol 80:3487–3494 [View Article][PubMed]
    [Google Scholar]
  24. Mu C., Yang Q., Zhang Y., Zhou Y., Zhang J., Martin D. P., Xia P., Cui B. 2012; Genetic variation and phylogenetic analysis of porcine circovirus type 2 infections in central China. Virus Genes 45:463–473 [View Article][PubMed]
    [Google Scholar]
  25. Nawagitgul P., Morozov I., Bolin S. R., Harms P. A., Sorden S. D., Paul P. S. 2000; Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J Gen Virol 81:2281–2287 [View Article][PubMed]
    [Google Scholar]
  26. Olvera A., Cortey M., Segalés J. 2007; Molecular evolution of porcine circovirus type 2 genomes: phylogeny and clonality. Virology 357:175–185 [View Article][PubMed]
    [Google Scholar]
  27. Opriessnig T., Xiao C. T., Gerber P. F., Halbur P. G. 2013; Emergence of a novel mutant PCV2b variant associated with clinical PCVAD in two vaccinated pig farms in the U.S. concurrently infected with PPV2. Vet Microbiol 163:177–183 [View Article][PubMed]
    [Google Scholar]
  28. Opriessnig T., Gerber P. F., Xiao C. T., Mogler M., Halbur P. G. 2014a; A commercial vaccine based on PCV2a and an experimental vaccine based on a variant mPCV2b are both effective in protecting pigs against challenge with a 2013 U.S. variant mPCV2b strain. Vaccine 32:230–237 [View Article][PubMed]
    [Google Scholar]
  29. Opriessnig T., Xiao C. T., Gerber P. F., Halbur P. G., Matzinger S. R., Meng X. J. 2014b; Mutant USA strain of porcine circovirus type 2 (mPCV2) exhibits similar virulence to the classical PCV2a and PCV2b strains in caesarean-derived, colostrum-deprived pigs. J Gen Virol 95:2495–2503 [View Article][PubMed]
    [Google Scholar]
  30. Pérez L. J., de Arce H. D., Cortey M., Domínguez P., Percedo M. I., Perera C. L., Tarradas J., Frías M. T., Segalés J., other authors. 2011; Phylogenetic networks to study the origin and evolution of porcine circovirus type 2 (PCV2) in Cuba. Vet Microbiol 151:245–254 [View Article][PubMed]
    [Google Scholar]
  31. Pogranichnyy R. M., Yoon K. J., Harms P. A., Swenson S. L., Zimmerman J. J., Sorden S. D. 2000; Characterization of immune response of young pigs to porcine circovirus type 2 infection. Viral Immunol 13:143–153 [View Article][PubMed]
    [Google Scholar]
  32. Reiner G., Hofmeister R., Willems H. 2015; Genetic variability of porcine circovirus 2 (PCV2) field isolates from vaccinated and non-vaccinated pig herds in Germany. Vet Microbiol 180:41–48 [View Article][PubMed]
    [Google Scholar]
  33. Segalés J. 2012; Porcine circovirus type 2 (PCV2) infections: clinical signs, pathology and laboratory diagnosis. Virus Res 164:10–19 [View Article][PubMed]
    [Google Scholar]
  34. Segalés J., Allan G. M., Domingo M. 2005; Porcine circovirus diseases. Anim Health Res Rev 6:119–142 [View Article][PubMed]
    [Google Scholar]
  35. Segalés J., Olvera A., Grau-Roma L., Charreyre C., Nauwynck H., Larsen L., Dupont K., McCullough K., Ellis J., other authors. 2008; PCV-2 genotype definition and nomenclature. Vet Rec 162:867–868 [View Article][PubMed]
    [Google Scholar]
  36. Segalés J., Kekarainen T., Cortey M. 2013; The natural history of porcine circovirus type 2: from an inoffensive virus to a devastating swine disease?. Vet Microbiol 165:13–20 [View Article][PubMed]
    [Google Scholar]
  37. Seo H. W., Park C., Kang I., Choi K., Jeong J., Park S. J., Chae C. 2014; Genetic and antigenic characterization of a newly emerging porcine circovirus type 2b mutant first isolated in cases of vaccine failure in Korea. Arch Virol 159:3107–3111 [View Article][PubMed]
    [Google Scholar]
  38. Shang S. B., Jin Y. L., Jiang X. T., Zhou J. Y., Zhang X., Xing G., He J. L., Yan Y. 2009; Fine mapping of antigenic epitopes on capsid proteins of porcine circovirus, and antigenic phenotype of porcine circovirus type 2. Mol Immunol 46:327–334 [View Article][PubMed]
    [Google Scholar]
  39. Suomalainen M., Greber U. F. 2013; Uncoating of non-enveloped viruses. Curr Opin Virol 3:27–33 [View Article][PubMed]
    [Google Scholar]
  40. Tischer I., Rasch R., Tochtermann G. 1974; Characterization of papovavirus- and picornavirus-like particles in permanent pig kidney cell lines. Zentralbl Bakteriol Orig A 226:153–167[PubMed]
    [Google Scholar]
  41. Tischer I., Mields W., Wolff D., Vagt M., Griem W. 1986; Studies on epidemiology and pathogenicity of porcine circovirus. Arch Virol 91:271–276 [View Article][PubMed]
    [Google Scholar]
  42. Trible B. R., Rowland R. R. 2012; Genetic variation of porcine circovirus type 2 (PCV2) and its relevance to vaccination, pathogenesis and diagnosis. Virus Res 164:68–77 [View Article][PubMed]
    [Google Scholar]
  43. Wang C., Pang V. F., Lee F., Huang T. S., Lee S. H., Lin Y. J., Lin Y. L., Lai S. S., Jeng C. R. 2013; Prevalence and genetic variation of porcine circovirus type 2 in Taiwan from 2001 to 2011. Res Vet Sci 94:789–795 [View Article][PubMed]
    [Google Scholar]
  44. Wei C., Zhang M., Chen Y., Xie J., Huang Z., Zhu W., Xu T., Cao Z., Zhou P., other authors. 2013; Genetic evolution and phylogenetic analysis of porcine circovirus type 2 infections in southern China from 2011 to 2012. Infect Genet Evol 17:87–92 [View Article][PubMed]
    [Google Scholar]
  45. Wiederkehr D. D., Sydler T., Buergi E., Haessig M., Zimmermann D., Pospischil A., Brugnera E., Sidler X. 2009; A new emerging genotype subgroup within PCV-2b dominates the PMWS epizooty in Switzerland. Vet Microbiol 136:27–35 [View Article][PubMed]
    [Google Scholar]
  46. Xiao C. T., Halbur P. G., Opriessnig T. 2012; Complete genome sequence of a novel porcine circovirus type 2b variant present in cases of vaccine failures in the United States. J Virol 86:12469 [View Article][PubMed]
    [Google Scholar]
  47. Zhai S. L., Chen S. N., Xu Z. H., Tang M. H., Wang F. G., Li X. J., Sun B. B., Deng S. F., Hu J., other authors. 2014; Porcine circovirus type 2 in China: an update on and insights to its prevalence and control. Virol J 11:88 [View Article][PubMed]
    [Google Scholar]
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