1887

Abstract

Pseudorabies virus is the causative agent of Aujeszky's disease. Domestic pigs and wild boars are its natural hosts, and strains circulating within both populations differ in their capacity to induce clinical disease. Cell biological and molecular explanations for the observed differences in virulence are, however, lacking. Different virulence determinants that can be assessed were determined for five domestic swine strains, four wild boar strains and the NIA3 reference strain. Replication kinetics and plaque formation capacity in continuous swine testicular cells and different primary porcine cell lines were highly similar for isolates from both populations. Treatment of these cell lines with IFNα, IFNγ or a combination of both provoked similar plaque-reducing effects for all strains. In conclusion, our results indicate that isolates from domestic swine and wild boar differ neither in intrinsic replication and dissemination capacity nor in sensitivity to antiviral effects of IFNs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000348
2016-02-01
2019-09-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/97/2/473.html?itemId=/content/journal/jgv/10.1099/jgv.0.000348&mimeType=html&fmt=ahah

References

  1. Brukman A., Enquist L. W.. ( 2006a;). Suppression of the interferon-mediated innate immune response by pseudorabies virus. J Virol 80: 6345–6356 [CrossRef] [PubMed].
    [Google Scholar]
  2. Brukman A., Enquist L. W.. ( 2006b;). Pseudorabies virus EP0 protein counteracts an interferon-induced antiviral state in a species-specific manner. J Virol 80: 10871–10873 [CrossRef] [PubMed].
    [Google Scholar]
  3. Czaplicki G., Dufey J., Saegerman C.. ( 2006;). Le Sanglier Wallon est-il un reservoir potential du virus de la maladie d'Aujeszky pour les elevages porcins?. Epidémiol Santé Anim 49: 89–101 (in French).
    [Google Scholar]
  4. De Regge N., Favoreel H. W., Geenen K., Nauwynck H. J.. ( 2006;). A homologous in vitro model to study interactions between alphaherpesviruses and trigeminal ganglion neurons. Vet Microbiol 113: 251–255 [CrossRef] [PubMed].
    [Google Scholar]
  5. De Regge N., Van Opdenbosch N., Nauwynck H. J., Efstathiou S., Favoreel H. W.. ( 2010;). Interferon alpha induces establishment of alphaherpesvirus latency in sensory neurons in vitro. PLoS One 5: e13076 [CrossRef] [PubMed].
    [Google Scholar]
  6. Glorieux S., Favoreel H. W., Meesen G., de Vos W., Van den Broeck W., Nauwynck H. J.. ( 2009;). Different replication characteristics of historical pseudorabies virus strains in porcine respiratory nasal mucosa explants. Vet Microbiol 136: 341–346 [CrossRef] [PubMed].
    [Google Scholar]
  7. Goldberg T. L., Weigel R. M., Hahn E. C., Scherba G.. ( 2001;). Comparative utility of restriction fragment length polymorphism analysis and gene sequencing to the molecular epidemiological investigation of a viral outbreak. Epidemiol Infect 126: 415–424 [CrossRef] [PubMed].
    [Google Scholar]
  8. Hahn E. C., Page G. R., Hahn P. S., Gillis K. D., Romero C., Annelli J. A., Gibbs E. P.. ( 1997;). Mechanisms of transmission of Aujeszky's disease virus originating from feral swine in the USA. Vet Microbiol 55: 123–130 [CrossRef] [PubMed].
    [Google Scholar]
  9. Hahn E. C., Fadl-Alla B., Lichtensteiger C. A.. ( 2010;). Variation of Aujeszky's disease viruses in wild swine in USA. Vet Microbiol 143: 45–51 [CrossRef] [PubMed].
    [Google Scholar]
  10. Klopfleisch R., Klupp B. G., Fuchs W., Kopp M., Teifke J. P., Mettenleiter T. C.. ( 2006;). Influence of pseudorabies virus proteins on neuroinvasion and neurovirulence in mice. J Virol 80: 5571–5576 [CrossRef] [PubMed].
    [Google Scholar]
  11. Klupp B. G., Granzow H., Mettenleiter T. C.. ( 2000;). Primary envelopment of pseudorabies virus at the nuclear membrane requires the UL34 gene product. J Virol 74: 10063–10073 [CrossRef] [PubMed].
    [Google Scholar]
  12. Müller T. F., Teuffert J., Zellmer R., Conraths F. J.. ( 2001;). Experimental infection of European wild boars and domestic pigs with pseudorabies viruses with differing virulence. Am J Vet Res 62: 252–258 [CrossRef] [PubMed].
    [Google Scholar]
  13. Müller T., Klupp B. G., Freuling C., Hoffmann B., Mojcicz M., Capua I., Palfi V., Toma B., Lutz W., other authors. ( 2010;). Characterization of pseudorabies virus of wild boar origin from Europe. Epidemiol Infect 138: 1590–1600 [CrossRef] [PubMed].
    [Google Scholar]
  14. Müller T., Hahn E. C., Tottewitz F., Kramer M., Klupp B. G., Mettenleiter T. C., Freuling C.. ( 2011;). Pseudorabies virus in wild swine: a global perspective. Arch Virol 156: 1691–1705 [CrossRef] [PubMed].
    [Google Scholar]
  15. Nauwynck H., Glorieux S., Favoreel H., Pensaert M.. ( 2007;). Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract. Vet Res 38: 229–241 [CrossRef] [PubMed].
    [Google Scholar]
  16. Pannwitz G., Freuling C., Denzin N., Schaarschmidt U., Nieper H., Hlinak A., Burkhardt S., Klopries M., Dedek J., other authors. ( 2012;). A long-term serological survey on Aujeszky's disease virus infections in wild boar in East Germany. Epidemiol Infect 140: 348–358 [CrossRef] [PubMed].
    [Google Scholar]
  17. Pomeranz L. E., Reynolds A. E., Hengartner C. J.. ( 2005;). Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine. Microbiol Mol Biol Rev 69: 462–500 [CrossRef] [PubMed].
    [Google Scholar]
  18. Van Minnebruggen G., Favoreel H. W., Jacobs L., Nauwynck H. J.. ( 2003;). Pseudorabies virus US3 protein kinase mediates actin stress fiber breakdown. J Virol 77: 9074–9080 [CrossRef] [PubMed].
    [Google Scholar]
  19. Van Opdenbosch N., De Regge N., Van Poucke M., Peelman L., Favoreel H. W.. ( 2011;). Effects of interferon on immediate-early mRNA and protein levels in sensory neuronal cells infected with herpes simplex virus type 1 or pseudorabies virus. Vet Microbiol 152: 401–406 [CrossRef] [PubMed].
    [Google Scholar]
  20. Vandekerckhove A. P., Glorieux S., Gryspeerdt A. C., Steukers L., Duchateau L., Osterrieder N., Van de Walle G. R., Nauwynck H. J.. ( 2010;). Replication kinetics of neurovirulent versus non-neurovirulent equine herpesvirus type 1 strains in equine nasal mucosal explants. J Gen Virol 91: 2019–2028 [CrossRef] [PubMed].
    [Google Scholar]
  21. Verpoest S., Cay A. B., De Regge N.. ( 2014;). Molecular characterization of Belgian pseudorabies virus isolates from domestic swine and wild boar. Vet Microbiol 172: 72–77 [CrossRef] [PubMed].
    [Google Scholar]
  22. Verpoest S., Cay A. B., Van Campe W., Mostin L., Welby S., Favoreel H., De Regge N.. ( 2016;). Age and strain dependent differences in the outcome of experimental infections of domestic pigs with Belgian wild boar pseudorabies virus isolates. J Gen Virol97 480–486 [CrossRef].
    [Google Scholar]
  23. Yao Q., Qian P., Cao Y., He Y., Si Y., Xu Z., Chen H.. ( 2007;). Synergistic inhibition of pseudorabies virus replication by porcine alpha/beta interferon and gamma interferon in vitro. Eur Cytokine Netw 18: 71–77 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000348
Loading
/content/journal/jgv/10.1099/jgv.0.000348
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error