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Journal of General Virology header logo Journal of General Virology

Volume 96, Issue 12

Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and agglutinin No Access

Abstract

Human immunodeficiency virus type 1 (HIV-1) transmission often results from infection by a single transmitted/founder (T/F) virus. Here, we investigated the sensitivity of T/F HIV-1 envelope glycoproteins (Envs) to microbicide candidate carbohydrate-binding agents (CBAs) griffithsin (GRFT), cyanovirin-N (CV-N) and agglutinin (GNA), showing that T/F Envs demonstrated different sensitivity to CBAs, with IC values ranging from 0.006 ± 0.0003 to >10 nM for GRFT, from 0.6 ± 0.2 to 28.9 ± 2.9 nM for CV-N and from 1.3 ± 0.2 to >500 nM for GNA. We further revealed that deglycosylation at position 295 or 448 decreased the sensitivity of T/F Env to GRFT, and at 339 to both CV-N and GNA. Mutation of all the three glcyans rendered a CBA-sensitive T/F Env largely resistant to GRFT, indicating that the sensitivity of T/F Env to GRFT is mainly determined by glycans at 295, 339 and 448. Our study identified specific T/F Env residues associated with CBA sensitivity.

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© 2015 The Authors
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/content/journal/jgv/10.1099/jgv.0.000299
2015-12-01
2025-12-11

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/content/journal/jgv/10.1099/jgv.0.000299
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Sensitivity of transmitted and founder human immunodeficiency virus type 1 envelopes to carbohydrate-binding agents griffithsin, cyanovirin-N and Galanthus nivalis agglutinin
J. Gen. Virol. 96, 3660 (2015); https://doi.org/10.1099/jgv.0.000299
/content/journal/jgv/10.1099/jgv.0.000299
/content/journal/jgv/10.1099/jgv.0.000299
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