@article{mbs:/content/journal/jgv/10.1099/jgv.0.000293, author = "Zahin, Maryam and Ghim, Shin-je and Khanal, Sujita and Bossart, Gregory D. and Jenson, Alfred B. and Joh, Joongho", title = "Molecular characterization of novel mucosotropic papillomaviruses from a Florida manatee (Trichechus manatus latirostris)", journal= "Journal of General Virology", year = "2015", volume = "96", number = "12", pages = "3545-3553", doi = "https://doi.org/10.1099/jgv.0.000293", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000293", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "We isolated two new manatee papillomavirus (PV) types, TmPV3 and TmPV4, from a Florida manatee (Trichechus manatus latirostris). Two PV types were previously isolated from this species. TmPV1 is widely dispersed amongst manatees and a close-to-root PV; not much is known about TmPV2. The genomes of TmPV3 and TmPV4 were 7622 and 7771 bp in size, respectively. Both PVs had a genomic organization characteristic of all PVs, with one non-coding region and seven ORFs, including the E7 ORF that is absent in other cetacean PVs. Although these PVs were isolated from separate genital lesions of the same manatee, an enlarged E2/E4 ORF was found only in the TmPV4 genome. The full genome and L1 sequence similarities between TmPV3 and TmPV4 were 63.2 and 70.3 %, respectively. These genomes shared only 49.1 and 50.2 % similarity with TmPV1. The pairwise alignment of L1 nucleotide sequences indicated that the two new PVs nested in a monophyletic group of the genus Rhopapillomavirus, together with the cutaneotropic TmPV1 and TmPV2.", }