RT Journal Article SR Electronic(1) A1 Berrone, Elena A1 Corona, Cristiano A1 Mazza, Maria A1 Costassa, Elena Vallino A1 Faro, Monica Lo A1 Properzi, Francesca A1 Guglielmetti, Chiara A1 Maurella, Cristiana A1 Caramelli, Maria A1 Deregibus, Maria Chiara A1 Camussi, Giovanni A1 Casalone, CristinaYR 2015 T1 Detection of cellular prion protein in exosomes derived from ovine plasma JF Journal of General Virology, VO 96 IS 12 SP 3698 OP 3702 DO https://doi.org/10.1099/jgv.0.000291 PB Microbiology Society, SN 1465-2099, AB Prion protein (PrP) is present at extremely low levels in the blood of animals and its detection is complicated by the poor sensitivity of current standard methodologies. Interesting results have been obtained with recent advanced technologies that are able to detect minute amounts of the pathological PrP (PrPSc), but their efficiency is reduced by various factors present in blood. In this study, we were able to extract cellular PrP (PrPC) from plasma-derived exosomes by a simple, fast method without the use of differential ultracentrifugation and to visualize it by Western blotting, reducing the presence of most plasma proteins. This result confirms that blood is capable of releasing PrP in association with exosomes and could be useful to better study its role in the pathogenesis of transmissible spongiform encephalopathies., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000291