@article{mbs:/content/journal/jgv/10.1099/jgv.0.000291, author = "Berrone, Elena and Corona, Cristiano and Mazza, Maria and Costassa, Elena Vallino and Faro, Monica Lo and Properzi, Francesca and Guglielmetti, Chiara and Maurella, Cristiana and Caramelli, Maria and Deregibus, Maria Chiara and Camussi, Giovanni and Casalone, Cristina", title = "Detection of cellular prion protein in exosomes derived from ovine plasma", journal= "Journal of General Virology", year = "2015", volume = "96", number = "12", pages = "3698-3702", doi = "https://doi.org/10.1099/jgv.0.000291", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000291", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Prion protein (PrP) is present at extremely low levels in the blood of animals and its detection is complicated by the poor sensitivity of current standard methodologies. Interesting results have been obtained with recent advanced technologies that are able to detect minute amounts of the pathological PrP (PrPSc), but their efficiency is reduced by various factors present in blood. In this study, we were able to extract cellular PrP (PrPC) from plasma-derived exosomes by a simple, fast method without the use of differential ultracentrifugation and to visualize it by Western blotting, reducing the presence of most plasma proteins. This result confirms that blood is capable of releasing PrP in association with exosomes and could be useful to better study its role in the pathogenesis of transmissible spongiform encephalopathies.", }