1887

Abstract

Borna disease virus (BDV) is a non-segmented, negative-strand RNA virus that establishes persistent infection in the nucleus. Although BDV forms viral inclusion bodies, termed viral speckles of transcripts (vSPOTs), which are associated with chromatin in the nucleus, the host factors involved in the maintenance of vSPOTs remain largely unknown. In this study, we identified X-linked RNA-binding motif protein (RBMX) as a nuclear factor interacting with BDV nucleoprotein. Interestingly, knockdown of RBMX led to disruption of the formation of vSPOTs and reduced both transcription and replication of BDV. Our results indicate that RBMX is involved in the maintenance of the structure of the virus factory in the nucleus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000273
2015-11-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/11/3198.html?itemId=/content/journal/jgv/10.1099/jgv.0.000273&mimeType=html&fmt=ahah

References

  1. Briese T., de la Torre J.C., Lewis A., Ludwig H., Lipkin W.I. 1992; Borna disease virus, a negative-strand RNA virus, transcribes in the nucleus of infected cells. Proc Natl Acad Sci U S A 89:11486–11489 [View Article][PubMed]
    [Google Scholar]
  2. Briese T., Schneemann A., Lewis A.J., Park Y.S., Kim S., Ludwig H., Lipkin W.I. 1994; Genomic organization of Borna disease virus. Proc Natl Acad Sci U S A 91:4362–4366 [View Article][PubMed]
    [Google Scholar]
  3. Chase G., Mayer D., Hildebrand A., Frank R., Hayashi Y., Tomonaga K., Schwemmle M. 2007; Borna disease virus matrix protein is an integral component of the viral ribonucleoprotein complex that does not interfere with polymerase activity. J Virol 81:743–749 [View Article][PubMed]
    [Google Scholar]
  4. Cubitt B., de la Torre J.C. 1994; Borna disease virus (BDV), a nonsegmented RNA virus, replicates in the nuclei of infected cells where infectious BDV ribonucleoproteins are present. J Virol 68:1371–1381[PubMed]
    [Google Scholar]
  5. Cubitt B., Oldstone C., de la Torre J.C. 1994a; Sequence and genome organization of Borna disease virus. J Virol 68:1382–1396[PubMed]
    [Google Scholar]
  6. Cubitt B., Oldstone C., Valcarcel J., de la Torre C.J. 1994b; RNA splicing contributes to the generation of mature mRNAs of Borna disease virus, a non-segmented negative strand RNA virus. Virus Res 34:69–79 [View Article][PubMed]
    [Google Scholar]
  7. Heinrich B., Zhang Z., Raitskin O., Hiller M., Benderska N., Hartmann A.M., Bracco L., Elliott D., Ben-Ari S., other authors. 2009; Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA. J Biol Chem 284:14303–14315 [View Article][PubMed]
    [Google Scholar]
  8. Herzog S., Rott R. 1980; Replication of Borna disease virus in cell cultures. Med Microbiol Immunol (Berl) 168:153–158 [View Article][PubMed]
    [Google Scholar]
  9. Hirai Y., Louvet E., Oda T., Kumeta M., Watanabe Y., Horigome T., Takeyasu K. 2013; Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21. Genes Cells 18:780–797 [View Article][PubMed]
    [Google Scholar]
  10. Hofmann Y., Wirth B. 2002; hnRNP-G promotes exon 7 inclusion of survival motor neuron (SMN) via direct interaction with Htra2-β1. Hum Mol Genet 11:2037–2049 [View Article][PubMed]
    [Google Scholar]
  11. Kamitani W., Shoya Y., Kobayashi T., Watanabe M., Lee B.J., Zhang G., Tomonaga K., Ikuta K. 2001; Borna disease virus phosphoprotein binds a neurite outgrowth factor, amphoterin/HMG-1. J Virol 75:8742–8751 [View Article][PubMed]
    [Google Scholar]
  12. Kobayashi T., Zhang G., Lee B.J., Baba S., Yamashita M., Kamitani W., Yanai H., Tomonaga K., Ikuta K. 2003; Modulation of Borna disease virus phosphoprotein nuclear localization by the viral protein X encoded in the overlapping open reading frame. J Virol 77:8099–8107 [View Article][PubMed]
    [Google Scholar]
  13. Matsumoto Y., Hayashi Y., Omori H., Honda T., Daito T., Horie M., Ikuta K., Fujino K., Nakamura S., other authors. 2012; Bornavirus closely associates and segregates with host chromosomes to ensure persistent intranuclear infection. Cell Host Microbe 11:492–503 [View Article][PubMed]
    [Google Scholar]
  14. Matsunaga S., Takata H., Morimoto A., Hayashihara K., Higashi T., Akatsuchi K., Mizusawa E., Yamakawa M., Ashida M., other authors. 2012; RBMX: a regulator for maintenance and centromeric protection of sister chromatid cohesion. Cell Reports 1:299–308 [View Article][PubMed]
    [Google Scholar]
  15. Nakamura Y., Takahashi H., Shoya Y., Nakaya T., Watanabe M., Tomonaga K., Iwahashi K., Ameno K., Momiyama N., other authors. 2000; Isolation of Borna disease virus from human brain tissue. J Virol 74:4601–4611 [View Article][PubMed]
    [Google Scholar]
  16. Nasim M.T., Chernova T.K., Chowdhury H.M., Yue B.G., Eperon I.C. 2003; HnRNP G and Tra2β: opposite effects on splicing matched by antagonism in RNA binding. Hum Mol Genet 12:1337–1348 [View Article][PubMed]
    [Google Scholar]
  17. Schneemann A., Schneider P.A., Lamb R.A., Lipkin W.I. 1995; The remarkable coding strategy of Borna disease virus: a new member of the nonsegmented negative strand RNA viruses. Virology 210:1–8 [View Article][PubMed]
    [Google Scholar]
  18. Schneider U. 2005; Novel insights into the regulation of the viral polymerase complex of neurotropic Borna disease virus. Virus Res 111:148–160 [View Article][PubMed]
    [Google Scholar]
  19. Schneider P.A., Schneemann A., Lipkin W.I. 1994; RNA splicing in Borna disease virus, a nonsegmented, negative-strand RNA virus. J Virol 68:5007–5012[PubMed]
    [Google Scholar]
  20. Tomonaga K., Kobayashi T., Lee B.J., Watanabe M., Kamitani W., Ikuta K. 2000; Identification of alternative splicing and negative splicing activity of a nonsegmented negative-strand RNA virus, Borna disease virus. Proc Natl Acad Sci U S A 97:12788–12793 [View Article][PubMed]
    [Google Scholar]
  21. Tomonaga K., Kobayashi T., Ikuta K. 2002; Molecular and cellular biology of Borna disease virus infection. Microbes Infect 4:491–500 [View Article][PubMed]
    [Google Scholar]
  22. Wang Y., Wang J., Gao L., Stamm S., Andreadis A. 2011; An SRp75/hnRNPG complex interacting with hnRNPE2 regulates the 5′ splice site of tau exon 10, whose misregulation causes frontotemporal dementia. Gene 485:130–138 [View Article][PubMed]
    [Google Scholar]
  23. Watanabe M., Zhong Q., Kobayashi T., Kamitani W., Tomonaga K., Ikuta K. 2000; Molecular ratio between Borna disease viral-p40 and -p24 proteins in infected cells determined by quantitative antigen capture ELISA. Microbiol Immunol 44:765–772 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000273
Loading
/content/journal/jgv/10.1099/jgv.0.000273
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error