1887

Abstract

Pestiviruses are some of the most significant pathogens affecting ruminants and swine. Here, we assembled a 11 276 bp contig encoding a predicted 3635 aa polyprotein from porcine serum with 68 % pairwise identity to that of a recently partially characterized Rhinolophus affinis pestivirus (RaPV) and approximately 25–28 % pairwise identity to those of other pestiviruses. The virus was provisionally named atypical porcine pestivirus (APPV). Metagenomic sequencing of 182 serum samples identified four additional APPV-positive samples. Positive samples originated from five states and ELISAs using recombinant APPV Erns found cross-reactive antibodies in 94 % of a collection of porcine serum samples, suggesting widespread distribution of APPV in the US swine herd. The molecular and serological results suggest that APPV is a novel, highly divergent porcine pestivirus widely distributed in US pigs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000251
2015-10-01
2020-04-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/10/2994.html?itemId=/content/journal/jgv/10.1099/jgv.0.000251&mimeType=html&fmt=ahah

References

  1. Becher P., Avalos Ramirez R., Orlich M., Cedillo Rosales S., König M., Schweizer M., Stalder H., Schirrmeier H., Thiel H.J. 2003; Genetic and antigenic characterization of novel pestivirus genotypes: implications for classification. Virology 311:96–104 [CrossRef][PubMed]
    [Google Scholar]
  2. Behrens S.E., Grassmann C.W., Thiel H.J., Meyers G., Tautz N. 1998; Characterization of an autonomous subgenomic pestivirus RNA replicon. J Virol 72:2364–2372[PubMed]
    [Google Scholar]
  3. Chander V., Nandi S., Ravishankar C., Upmanyu V., Verma R. 2014; Classical swine fever in pigs: recent developments and future perspectives. Anim Health Res Rev 15:87–101 [CrossRef][PubMed]
    [Google Scholar]
  4. Firth C., Bhat M., Firth M.A., Williams S.H., Frye M.J., Simmonds P., Conte J.M., Ng J., Garcia J., other authors. 2014; Detection of zoonotic pathogens and characterization of novel viruses carried by commensal Rattus norvegicus in New York city. MBio 5:e01933–e01914 [CrossRef][PubMed]
    [Google Scholar]
  5. Gladue D.P., Holinka L.G., Largo E., Fernandez Sainz I., Carrillo C., O'Donnell V., Baker-Branstetter R., Lu Z., Ambroggio X., other authors. 2012; Classical swine fever virus p7 protein is a viroporin involved in virulence in swine. J Virol 86:6778–6791 [CrossRef][PubMed]
    [Google Scholar]
  6. Hause B.M., Collin E.A., Anderson J., Hesse R.A., Anderson G. 2015; Bovine rhinitis viruses are common in U.S. cattle with bovine respiratory disease. PLoS One 10:e0121998 [CrossRef][PubMed]
    [Google Scholar]
  7. Iqbal M., Poole E., Goodbourn S., McCauley J.W. 2004; Role for bovine viral diarrhea virus Erns glycoprotein in the control of activation of beta interferon by double-stranded RNA. J Virol 78:136–145 [CrossRef][PubMed]
    [Google Scholar]
  8. Ivanyi-Nagy R., Lavergne J.P., Gabus C., Ficheux D., Darlix J.L. 2008; RNA chaperoning and intrinsic disorder in the core proteins of Flaviviridae . Nucleic Acids Res 36:712–725 [CrossRef][PubMed]
    [Google Scholar]
  9. Kirkland P.D., Frost M.J., Finlaison D.S., King K.R., Ridpath J.F., Gu X. 2007; Identification of a novel virus in pigs—Bungowannah virus: a possible new species of pestivirus. Virus Res 129:26–34 [CrossRef][PubMed]
    [Google Scholar]
  10. Lackner T., Müller A., Pankraz A., Becher P., Thiel H.J., Gorbalenya A.E., Tautz N. 2004; Temporal modulation of an autoprotease is crucial for replication and pathogenicity of an RNA virus. J Virol 78:10765–10775 [CrossRef][PubMed]
    [Google Scholar]
  11. Lamp B., Riedel C., Wentz E., Tortorici M.A., Rümenapf T. 2013; Autocatalytic cleavage within classical swine fever virus NS3 leads to a functional separation of protease and helicase. J Virol 87:11872–11883 [CrossRef][PubMed]
    [Google Scholar]
  12. Liang D., Sainz I.F., Ansari I.H., Gil L.H.V.G., Vassilev V., Donis R.O. 2003; The envelope glycoprotein E2 is a determinant of cell culture tropism in ruminant pestiviruses. J Gen Virol 84:1269–1274 [CrossRef][PubMed]
    [Google Scholar]
  13. Lin M., Trottier E., Pasick J. 2005; Antibody responses of pigs to defined Erns fragments after infection with classical swine fever virus. Clin Diagn Lab Immunol 12:180–186[PubMed]
    [Google Scholar]
  14. Maurer K., Krey T., Moennig V., Thiel H.J., Rümenapf T. 2004; CD46 is a cellular receptor for bovine viral diarrhea virus. J Virol 78:1792–1799 [CrossRef][PubMed]
    [Google Scholar]
  15. Murray C.L., Marcotrigiano J., Rice C.M. 2008; Bovine viral diarrhea virus core is an intrinsically disordered protein that binds RNA. J Virol 82:1294–1304 [CrossRef][PubMed]
    [Google Scholar]
  16. Neill J.D., Bayles D.O., Ridpath J.F. 2014; Simultaneous rapid sequencing of multiple RNA virus genomes. J Virol Methods 201:68–72 [CrossRef][PubMed]
    [Google Scholar]
  17. Origgi F.C., Klein P.A., Mathes K., Blahak S., Marschang R.E., Tucker S.J., Jacobson E.R. 2001; Enzyme-linked immunosorbent assay for detecting herpesvirus exposure in Mediterranean tortoises (spur-thighed tortoise [Testudo graeca] and Hermann's tortoise [Testudo hermanni]). J Clin Microbiol 39:3156–3163 [CrossRef][PubMed]
    [Google Scholar]
  18. Ridpath J.F., Bolin S.R. 1997; Comparison of the complete genomic sequence of the border disease virus, BD31, to other pestiviruses. Virus Res 50:237–243 [CrossRef][PubMed]
    [Google Scholar]
  19. Ronecker S., Zimmer G., Herrler G., Greiser-Wilke I., Grummer B. 2008; Formation of bovine viral diarrhea virus E1-E2 heterodimers is essential for virus entry and depends on charged residues in the transmembrane domains. J Gen Virol 89:2114–2121 [CrossRef][PubMed]
    [Google Scholar]
  20. Rümenapf T., Unger G., Strauss J.H., Thiel H.J. 1993; Processing of the envelope glycoproteins of pestiviruses. J Virol 67:3288–3294[PubMed]
    [Google Scholar]
  21. Rümenapf T., Stark R., Heimann M., Thiel H.J. 1998; N-terminal protease of pestiviruses: identification of putative catalytic residues by site-directed mutagenesis. J Virol 72:2544–2547[PubMed]
    [Google Scholar]
  22. Ryan M.D., Monaghan S., Flint M. 1998; Virus-encoded proteinases of the Flaviviridae . J Gen Virol 79:947–959[PubMed] [CrossRef]
    [Google Scholar]
  23. Schneider R., Unger G., Stark R., Schneider-Scherzer E., Thiel H.J. 1993; Identification of a structural glycoprotein of an RNA virus as a ribonuclease. Science 261:1169–1171 [CrossRef][PubMed]
    [Google Scholar]
  24. Simmonds P., Becher P., Collett M.S., Gould E.A., Heinz F.X., Meyers G., Monath T., Pletnev A., Rice C.M., other authors. 2011; Family Flaviviridae. In Virus Taxonomy-Ninth Report of the International Committee on the Taxonomy of Viruses pp. 1003–1020 Edited by King A. M. Q., Adams M. J., Carstens E. B., Lefkowitz E. J. San Diego, CA: Elsevier Academic Press;
    [Google Scholar]
  25. Stark R., Meyers G., Rümenapf T., Thiel H.J. 1993; Processing of pestivirus polyprotein: cleavage site between autoprotease and nucleocapsid protein of classical swine fever virus. J Virol 67:7088–7095[PubMed]
    [Google Scholar]
  26. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  27. Tao J., Liao J., Wang Y., Zhang X., Wang J., Zhu G. 2013; Bovine viral diarrhea virus (BVDV) infections in pigs. Vet Microbiol 165:185–189 [CrossRef][PubMed]
    [Google Scholar]
  28. Tijssen P., Pellerin C., Lecomte J., van den Hurk J. 1996; Immunodominant E2 (gp53) sequences of highly virulent bovine viral diarrhea group II viruses indicate a close resemblance to a subgroup of border disease viruses. Virology 217:356–361 [CrossRef][PubMed]
    [Google Scholar]
  29. Vilcek S., Ridpath J.F., Van Campen H., Cavender J.L., Warg J. 2005; Characterization of a novel pestivirus originating from a pronghorn antelope. Virus Res 108:187–193 [CrossRef][PubMed]
    [Google Scholar]
  30. Wu Z., Ren X., Yang L., Hu Y., Yang J., He G., Zhang J., Dong J., Sun L., other authors. 2012; Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces. J Virol 86:10999–11012 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000251
Loading
/content/journal/jgv/10.1099/jgv.0.000251
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error