RT Journal Article SR Electronic(1) A1 Demange, Antonin A1 Yajjou-Hamalian, Halima A1 Gallay, Kathy A1 Luengo, Catherine A1 Beven, Véronique A1 Leroux, Aurélie A1 Confort, Marie-Pierre A1 Al Andary, Elsy A1 Gouet, Patrice A1 Moreau, Karen A1 Ronfort, Corinne A1 Blanchard, YannickYR 2015 T1 Porcine endogenous retrovirus-A/C: biochemical properties of its integrase and susceptibility to raltegravir JF Journal of General Virology, VO 96 IS 10 SP 3124 OP 3130 DO https://doi.org/10.1099/jgv.0.000236 PB Microbiology Society, SN 1465-2099, AB Porcine endogenous retroviruses (PERVs) are present in the genomes of pig cells. The PERV-A/C recombinant virus can infect human cells and is a major risk of zoonotic disease in the case of xenotransplantation of pig organs to humans. Raltegravir (RAL) is a viral integrase (IN) inhibitor used in highly active antiretroviral treatment. In the present study, we explored the potential use of RAL against PERV-A/C. We report (i) a three-dimensional model of the PERV-A/C intasome complexed with RAL, (ii) the sensitivity of PERV-A/C IN to RAL in vitro and (iii) the sensitivity of a PERV-A/C-IRES-GFP recombinant virus to RAL in cellulo. We demonstrated that RAL is a potent inhibitor against PERV-A/C IN and PERV-A/C replication with IC50s in the nanomolar range. To date, the use of retroviral inhibitors remains the only way to control the risk of zoonotic PERV infection during pig-to-human xenotransplantation., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000236