@article{mbs:/content/journal/jgv/10.1099/jgv.0.000236, author = "Demange, Antonin and Yajjou-Hamalian, Halima and Gallay, Kathy and Luengo, Catherine and Beven, Véronique and Leroux, Aurélie and Confort, Marie-Pierre and Al Andary, Elsy and Gouet, Patrice and Moreau, Karen and Ronfort, Corinne and Blanchard, Yannick", title = "Porcine endogenous retrovirus-A/C: biochemical properties of its integrase and susceptibility to raltegravir", journal= "Journal of General Virology", year = "2015", volume = "96", number = "10", pages = "3124-3130", doi = "https://doi.org/10.1099/jgv.0.000236", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000236", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Porcine endogenous retroviruses (PERVs) are present in the genomes of pig cells. The PERV-A/C recombinant virus can infect human cells and is a major risk of zoonotic disease in the case of xenotransplantation of pig organs to humans. Raltegravir (RAL) is a viral integrase (IN) inhibitor used in highly active antiretroviral treatment. In the present study, we explored the potential use of RAL against PERV-A/C. We report (i) a three-dimensional model of the PERV-A/C intasome complexed with RAL, (ii) the sensitivity of PERV-A/C IN to RAL in vitro and (iii) the sensitivity of a PERV-A/C-IRES-GFP recombinant virus to RAL in cellulo. We demonstrated that RAL is a potent inhibitor against PERV-A/C IN and PERV-A/C replication with IC50s in the nanomolar range. To date, the use of retroviral inhibitors remains the only way to control the risk of zoonotic PERV infection during pig-to-human xenotransplantation.", }