RT Journal Article SR Electronic(1) A1 Wang, Lina A1 Yin, Zifei A1 Wang, Yuan A1 Lu, Yuan A1 Zhang, Daniel A1 Srivastava, Arun A1 Ling, Changquan A1 Aslanidi, George V. A1 Ling, ChenYR 2015 T1 Productive life cycle of adeno-associated virus serotype 2 in the complete absence of a conventional polyadenylation signal JF Journal of General Virology, VO 96 IS 9 SP 2780 OP 2787 DO https://doi.org/10.1099/jgv.0.000229 PB Microbiology Society, SN 1465-2099, AB We showed that WT adeno-associated virus serotype 2 (AAV2) genome devoid of a conventional polyadenylation [poly(A)] signal underwent complete genome replication, encapsidation and progeny virion production in the presence of adenovirus. The infectivity of the progeny virion was also retained. Using recombinant AAV2 vectors devoid of a human growth hormone poly(A) signal, we also demonstrated that a subset of mRNA transcripts contained the inverted terminal repeat (ITR) sequence at the 3′ end, which we designated ITR in RNA (ITRR). Furthermore, AAV replication (Rep) proteins were able to interact with the ITRR. Taken together, our studies suggest a new function of the AAV2 ITR as an RNA element to mediate transgene expression from poly(A)-deleted mRNA., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000229