1887

Abstract

KU80 is well-known as a key component of the non-homologous end-joining pathway used to repair DNA double-strand breaks. In addition, the KU80-containing DNA-dependent protein kinase complex in mammals can act as a cytoplasmic sensor for viral DNA to activate innate immune response. We have now, to our knowledge for the first time, demonstrated that the speed of a systemic infection with a plant DNA geminivirus in is KU80-dependent. The early emergence of Euphorbia yellow mosaic virus DNA was significantly increased in knockout mutants compared with wild-type sibling controls. The possible impact of KU80 on geminivirus multiplication by generating non-productive viral DNAs or its role as a pattern-recognition receptor against DNA virus infection is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000224
2015-09-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/9/2913.html?itemId=/content/journal/jgv/10.1099/jgv.0.000224&mimeType=html&fmt=ahah

References

  1. Ascencio-Ibáñez J.T. , Sozzani R. , Lee T.J. , Chu T.M. , Wolfinger R.D. , Cella R. , Hanley-Bowdoin L. . ( 2008;). Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol 148: 436–454 [CrossRef] [PubMed].
    [Google Scholar]
  2. Ferguson B.J. , Mansur D.S. , Peters N.E. , Ren H. , Smith G.L. . ( 2012;). DNA-PK is a DNA sensor for IRF-3-dependent innate immunity. eLife 1: e00047 [CrossRef] [PubMed].
    [Google Scholar]
  3. Gallego M.E. , Bleuyard J.Y. , Daoudal-Cotterell S. , Jallut N. , White C.I. . ( 2003a;). Ku80 plays a role in non-homologous recombination but is not required for T-DNA integration in Arabidopsis . Plant J 35: 557–565 [CrossRef] [PubMed].
    [Google Scholar]
  4. Gallego M.E. , Jalut N. , White C.I. . ( 2003b;). Telomerase dependence of telomere lengthening in Ku80 mutant Arabidopsis . Plant Cell 15: 782–789 [CrossRef] [PubMed].
    [Google Scholar]
  5. Glazov E. , Phillips K. , Budziszewski G.J. , Meins F. Jr , Levin J.Z. . ( 2003;). A gene encoding an RNase D exonuclease-like protein is required for post-transcriptional silencing in Arabidopsis . Plant J 35: 342–349 [CrossRef] [PubMed].
    [Google Scholar]
  6. Grundy G.J. , Moulding H.A. , Caldecott K.W. , Rulten S.L. . ( 2014;). One ring to bring them all—the role of Ku in mammalian non-homologous end joining. DNA Repair (Amst) 17: 30–38 [CrossRef] [PubMed].
    [Google Scholar]
  7. Hanley-Bowdoin L. , Bejarano E.R. , Robertson D. , Mansoor S. . ( 2013;). Geminiviruses: masters at redirecting and reprogramming plant processes. Nat Rev Microbiol 11: 777–788 [CrossRef] [PubMed].
    [Google Scholar]
  8. Hartung F. , Puchta H. . ( 2006;). The RecQ gene family in plants. J Plant Physiol 163: 287–296 [CrossRef] [PubMed].
    [Google Scholar]
  9. Hartung F. , Plchová H. , Puchta H. . ( 2000;). Molecular characterisation of RecQ homologues in Arabidopsis thaliana . Nucleic Acids Res 28: 4275–4282 [CrossRef] [PubMed] [PubMed].
    [Google Scholar]
  10. Hipp K. , Rau P. , Schäfer B. , Gronenborn B. , Jeske H. . ( 2014;). The RXL motif of the African cassava mosaic virus Rep protein is necessary for rereplication of yeast DNA and viral infection in plants. Virology 462-463: 189–198 [CrossRef] [PubMed].
    [Google Scholar]
  11. Jeske H. . ( 2007;). Replication of geminiviruses and the use of rolling circle amplification for their diagnosis. . In Tomato yellow leaf curl virus disease, pp. 141–156. Edited by Czosnek H. . Dordrecht: Springer; [CrossRef].
    [Google Scholar]
  12. Jeske H. . ( 2009;). Geminiviruses. . In Torque Teno Virus: The Still Elusive Human Pathogens, pp. 185–226. Edited by zur Hausen H. , de Villiers E.-M. . Berlin: Springer;.
    [Google Scholar]
  13. Jeske H. , Lütgemeier M. , Preiss W. . ( 2001;). DNA forms indicate rolling circle and recombination-dependent replication of Abutilon mosaic virus. EMBO J 20: 6158–6167 [CrossRef] [PubMed].
    [Google Scholar]
  14. Ketting R.F. , Haverkamp T.H. , van Luenen H.G. , Plasterk R.H. . ( 1999;). Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell 99: 133–141 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kittelmann K. , Rau P. , Gronenborn B. , Jeske H. . ( 2009;). Plant geminivirus Rep protein induces rereplication in fission yeast. J Virol 83: 6769–6778 [CrossRef] [PubMed].
    [Google Scholar]
  16. Knoll A. , Puchta H. . ( 2011;). The role of DNA helicases and their interaction partners in genome stability and meiotic recombination in plants. J Exp Bot 62: 1565–1579 [CrossRef] [PubMed].
    [Google Scholar]
  17. Kobbe D. , Blanck S. , Demand K. , Focke M. , Puchta H. . ( 2008;). AtRECQ2, a RecQ helicase homologue from Arabidopsis thaliana, is able to disrupt various recombinogenic DNA structures in vitro . Plant J 55: 397–405 [CrossRef] [PubMed].
    [Google Scholar]
  18. Li B. , Conway N. , Navarro S. , Comai L. , Comai L. . ( 2005;). A conserved and species-specific functional interaction between the Werner syndrome-like exonuclease atWEX and the Ku heterodimer in Arabidopsis . Nucleic Acids Res 33: 6861–6867 [CrossRef] [PubMed].
    [Google Scholar]
  19. Mannuss A. , Trapp O. , Puchta H. . ( 2012;). Gene regulation in response to DNA damage. Biochim Biophys Acta 1819: 154–165 [CrossRef] [PubMed].
    [Google Scholar]
  20. Nagar S. , Hanley-Bowdoin L. , Robertson D. . ( 2002;). Host DNA replication is induced by geminivirus infection of differentiated plant cells. Plant Cell 14: 2995–3007 [CrossRef] [PubMed].
    [Google Scholar]
  21. Paprotka T. , Deuschle K. , Pilartz M. , Jeske H. . ( 2015;). Form follows function in geminiviral minichromosome architecture. Virus Res 196: 44–55 [CrossRef] [PubMed].
    [Google Scholar]
  22. Plchova H. , Hartung F. , Puchta H. . ( 2003;). Biochemical characterization of an exonuclease from Arabidopsis thaliana reveals similarities to the DNA exonuclease of the human Werner syndrome protein. J Biol Chem 278: 44128–44138 [CrossRef] [PubMed].
    [Google Scholar]
  23. Preiss W. , Jeske H. . ( 2003;). Multitasking in replication is common among geminiviruses. J Virol 77: 2972–2980 [CrossRef] [PubMed].
    [Google Scholar]
  24. Puchta H. . ( 2005;). The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution. J Exp Bot 56: 1–14 [PubMed].[CrossRef]
    [Google Scholar]
  25. Richter K.S. , Kleinow T. , Jeske H. . ( 2014;). Somatic homologous recombination in plants is promoted by a geminivirus in a tissue-selective manner. Virology 452-453: 287–296 [CrossRef] [PubMed].
    [Google Scholar]
  26. Richter K.S. , Ende L. , Jeske H. . ( 2015;). Rad54 is not essential for any geminiviral replication mode in planta . Plant Mol Biol 87: 193–202 [CrossRef] [PubMed].
    [Google Scholar]
  27. Szittya G. , Burgyán J. . ( 2013;). RNA interference-mediated intrinsic antiviral immunity in plants. Curr Top Microbiol Immunol 371: 153–181 [PubMed].
    [Google Scholar]
  28. Tamura K. , Adachi Y. , Chiba K. , Oguchi K. , Takahashi H. . ( 2002;). Identification of Ku70 and Ku80 homologues in Arabidopsis thaliana: evidence for a role in the repair of DNA double-strand breaks. Plant J 29: 771–781 [CrossRef] [PubMed].
    [Google Scholar]
  29. Tops B.B. , Tabara H. , Sijen T. , Simmer F. , Mello C.C. , Plasterk R.H. , Ketting R.F. . ( 2005;). RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans . Nucleic Acids Res 33: 347–355 [CrossRef] [PubMed].
    [Google Scholar]
  30. Trinks D. , Rajeswaran R. , Shivaprasad P.V. , Akbergenov R. , Oakeley E.J. , Veluthambi K. , Hohn T. , Pooggin M.M. . ( 2005;). Suppression of RNA silencing by a geminivirus nuclear protein, AC2, correlates with transactivation of host genes. J Virol 79: 2517–2527 [CrossRef] [PubMed].
    [Google Scholar]
  31. West C.E. , Waterworth W.M. , Story G.W. , Sunderland P.A. , Jiang Q. , Bray C.M. . ( 2002;). Disruption of the Arabidopsis AtKu80 gene demonstrates an essential role for AtKu80 protein in efficient repair of DNA double-strand breaks in vivo . Plant J 31: 517–528 [CrossRef] [PubMed].
    [Google Scholar]
  32. Zellinger B. , Akimcheva S. , Puizina J. , Schirato M. , Riha K. . ( 2007;). Ku suppresses formation of telomeric circles and alternative telomere lengthening in Arabidopsis . Mol Cell 27: 163–169 [CrossRef] [PubMed].
    [Google Scholar]
  33. Zipfel C. . ( 2014;). Plant pattern-recognition receptors. Trends Immunol 35: 345–351 [CrossRef] [PubMed].
    [Google Scholar]
  34. Zvereva A.S. , Pooggin M.M. . ( 2012;). Silencing and innate immunity in plant defense against viral and non-viral pathogens. Viruses 4: 2578–2597 [CrossRef].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000224
Loading
/content/journal/jgv/10.1099/jgv.0.000224
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error