1887

Abstract

We previously showed that a mutated PB1 gene improved the growth kinetics of a H3N2 influenza reassortant. Here, we showed that the same mutations improved the growth kinetics of a virus containing the A/Vietnam/1203/2004 (H5N1) haemagglutinin and neuraminidase (NA). Total protein yield and NA activity were increased when a chimeric NA was included. These increases indicated that the synergistic effect was due to the gene constellation containing both the altered PB1 gene and the chimeric NA gene.

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2015-04-01
2020-01-22
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References

  1. Abt M., de Jonge J., Laue M., Wolff T.. ( 2011;). Improvement of H5N1 influenza vaccine viruses: influence of internal gene segments of avian and human origin on production and hemagglutinin content. . Vaccine 29:, 5153–5162. [CrossRef][PubMed]
    [Google Scholar]
  2. Adamo J. E., Liu T., Schmeisser F., Ye Z.. ( 2009;). Optimizing viral protein yield of influenza virus strain A/Vietnam/1203/2004 by modification of the neuraminidase gene. . J Virol 83:, 4023–4029. [CrossRef][PubMed]
    [Google Scholar]
  3. Baigent S. J., McCauley J. W.. ( 2001;). Glycosylation of haemagglutinin and stalk-length of neuraminidase combine to regulate the growth of avian influenza viruses in tissue culture. . Virus Res 79:, 177–185. [CrossRef][PubMed]
    [Google Scholar]
  4. Bourret V., Lyall J., Ducatez M. F., Guérin J. L., Tiley L.. ( 2012;). Development of an improved polykaryon-based influenza virus rescue system. . BMC Biotechnol 12:, 69. [CrossRef][PubMed]
    [Google Scholar]
  5. Cobbin J. C., Verity E. E., Gilbertson B. P., Rockman S. P., Brown L. E.. ( 2013;). The source of the PB1 gene in influenza vaccine reassortants selectively alters the hemagglutinin content of the resulting seed virus. . J Virol 87:, 5577–5585. [CrossRef][PubMed]
    [Google Scholar]
  6. Giesendorf B., Bosch F. X., Orlich M., Scholtissek C., Rott R.. ( 1986;). Studies on the temperature sensitivity of influenza A virus reassortants nonpathogenic for chicken. . Virus Res 5:, 27–42. [CrossRef][PubMed]
    [Google Scholar]
  7. Harvey R., Hamill M., Robertson J. S., Minor P. D., Vodeiko G. M., Weir J. P., Takahashi H., Harada Y., Itamura S.. & other authors ( 2012;). Application of deglycosylation to SDS PAGE analysis improves calibration of influenza antigen standards. . Biologicals 40:, 96–99. [CrossRef][PubMed]
    [Google Scholar]
  8. Hoffmann E., Neumann G., Kawaoka Y., Hobom G., Webster R. G.. ( 2000;). A DNA transfection system for generation of influenza A virus from eight plasmids. . Proc Natl Acad Sci U S A 97:, 6108–6113. [CrossRef][PubMed]
    [Google Scholar]
  9. Jing X., Phy K., Li X., Ye Z.. ( 2012;). Increased hemagglutinin content in a reassortant 2009 pandemic H1N1 influenza virus with chimeric neuraminidase containing donor A/Puerto Rico/8/34 virus transmembrane and stalk domains. . Vaccine 30:, 4144–4152. [CrossRef][PubMed]
    [Google Scholar]
  10. Li O. T., Chan M. C., Leung C. S., Chan R. W., Guan Y., Nicholls J. M., Poon L. L.. ( 2009;). Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation. . PLoS One 4:, e5658. [CrossRef][PubMed]
    [Google Scholar]
  11. Macken C. A., Webby R. J., Bruno W. J.. ( 2006;). Genotype turnover by reassortment of replication complex genes from avian influenza A virus. . J Gen Virol 87:, 2803–2815. [CrossRef][PubMed]
    [Google Scholar]
  12. Mitnaul L. J., Matrosovich M. N., Castrucci M. R., Tuzikov A. B., Bovin N. V., Kobasa D., Kawaoka Y.. ( 2000;). Balanced hemagglutinin and neuraminidase activities are critical for efficient replication of influenza A virus. . J Virol 74:, 6015–6020. [CrossRef][PubMed]
    [Google Scholar]
  13. Ngai K. L., Chan M. C., Chan P. K.. ( 2013;). Replication and transcription activities of ribonucleoprotein complexes reconstituted from avian H5N1, H1N1pdm09 and H3N2 influenza A viruses. . PLoS One 8:, e65038. [CrossRef][PubMed]
    [Google Scholar]
  14. Pan W., Dong Z., Meng W., Zhang W., Li T., Li C., Zhang B., Chen L.. ( 2012;). Improvement of influenza vaccine strain A/Vietnam/1194/2004 (H5N1) growth with the neuraminidase packaging sequence from A/Puerto Rico/8/34. . Hum Vaccin Immunother 8:, 252–259. [CrossRef][PubMed]
    [Google Scholar]
  15. Plant E. P., Liu T. M., Xie H., Ye Z.. ( 2012;). Mutations to A/Puerto Rico/8/34 PB1 gene improves seasonal reassortant influenza A virus growth kinetics. . Vaccine 31:, 207–212. [CrossRef][PubMed]
    [Google Scholar]
  16. Rudneva I. A., Timofeeva T. A., Shilov A. A., Kochergin-Nikitsky K. S., Varich N. L., Ilyushina N. A., Gambaryan A. S., Krylov P. S., Kaverin N. V.. ( 2007;). Effect of gene constellation and postreassortment amino acid change on the phenotypic features of H5 influenza virus reassortants. . Arch Virol 152:, 1139–1145. [CrossRef][PubMed]
    [Google Scholar]
  17. Sultana I., Gao J., Markoff L., Eichelberger M. C.. ( 2011;). Influenza neuraminidase-inhibiting antibodies are induced in the presence of zanamivir. . Vaccine 29:, 2601–2606. [CrossRef][PubMed]
    [Google Scholar]
  18. Varich N. L., Gitelman A. K., Shilov A. A., Smirnov Y. A., Kaverin N. V.. ( 2008;). Deviation from the random distribution pattern of influenza A virus gene segments in reassortants produced under non-selective conditions. . Arch Virol 153:, 1149–1154. [CrossRef][PubMed]
    [Google Scholar]
  19. Wagner R., Wolff T., Herwig A., Pleschka S., Klenk H. D.. ( 2000;). Interdependence of hemagglutinin glycosylation and neuraminidase as regulators of influenza virus growth: a study by reverse genetics. . J Virol 74:, 6316–6323. [CrossRef][PubMed]
    [Google Scholar]
  20. Wanitchang A., Kramyu J., Jongkaewwattana A.. ( 2010;). Enhancement of reverse genetics-derived swine-origin H1N1 influenza virus seed vaccine growth by inclusion of indigenous polymerase PB1 protein. . Virus Res 147:, 145–148. [CrossRef][PubMed]
    [Google Scholar]
  21. Wille M., Tolf C., Avril A., Latorre-Margalef N., Wallerström S., Olsen B., Waldenström J.. ( 2013;). Frequency and patterns of reassortment in natural influenza A virus infection in a reservoir host. . Virology 443:, 150–160. [CrossRef][PubMed]
    [Google Scholar]
  22. Zhou H., Yu Z., Hu Y., Tu J., Zou W., Peng Y., Zhu J., Li Y., Zhang A.. & other authors ( 2009;). The special neuraminidase stalk-motif responsible for increased virulence and pathogenesis of H5N1 influenza A virus. . PLoS One 4:, e6277. [CrossRef][PubMed]
    [Google Scholar]
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