1887

Abstract

Chikungunya virus (CHIKV) is a mosquito-borne pathogen responsible for epidemics of debilitating arthritic disease. The recent outbreak (2004–2014) resulted in an estimated 1.4–6.5 million cases, with imported cases reported in nearly 40 countries. The development of CHIKV-specific diagnostics and research tools is thus highly desirable. Herein we describe the generation and characterization of the first mAbs specific for the capsid protein (CP) of CHIKV. The antibodies recognized isolates representing the major genotypes of CHIKV, as well as several other alphaviruses, and were reactive in a range of assays including ELISA, Western blot, immunofluorescence and immunohistochemistry (IHC). We have also used the anti-CP mAb 5.5G9 in IHC studies to show that capsid antigen is persistently expressed 30 days post-infection in cells with macrophage morphology in a mouse model of chronic CHIKV disease. These antibodies may thus represent useful tools for further research, including investigations into the structure and function of CHIKV CP, and as valuable reagents for CHIKV detection in a range of settings.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000002
2015-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/3/507.html?itemId=/content/journal/jgv/10.1099/jgv.0.000002&mimeType=html&fmt=ahah

References

  1. Borgherini G., Poubeau P., Staikowsky F., Lory M., Le Moullec N., Becquart J. P., Wengling C., Michault A., Paganin F. 2007; Outbreak of chikungunya on Reunion Island: early clinical and laboratory features in 157 adult patients. Clin Infect Dis 44:1401–1407 [View Article][PubMed]
    [Google Scholar]
  2. Choi H. K., Tong L., Minor W., Dumas P., Boege U., Rossmann M. G., Wengler G. 1991; Structure of Sindbis virus core protein reveals a chymotrypsin-like serine proteinase and the organization of the virion. Nature 354:37–43 [View Article][PubMed]
    [Google Scholar]
  3. Clark D. C., Lobigs M., Lee E., Howard M. J., Clark K., Blitvich B. J., Hall R. A. 2007; In situ reactions of monoclonal antibodies with a viable mutant of Murray Valley encephalitis virus reveal an absence of dimeric NS1 protein. J Gen Virol 88:1175–1183 [View Article][PubMed]
    [Google Scholar]
  4. Economopoulou A., Dominguez M., Helynck B., Sissoko D., Wichmann O., Quenel P., Germonneau P., Quatresous I. 2009; Atypical chikungunya virus infections: clinical manifestations, mortality and risk factors for severe disease during the 2005–2006 outbreak on Réunion. Epidemiol Infect 137:534–541 [View Article][PubMed]
    [Google Scholar]
  5. Gardner J., Anraku I., Le T. T., Larcher T., Major L., Roques P., Schroder W. A., Higgs S., Suhrbier A. 2010; Chikungunya virus arthritis in adult wild-type mice. J Virol 848021–8032 [CrossRef]
    [Google Scholar]
  6. Goh L. Y., Hobson-Peters J., Prow N. A., Gardner J., Bielefeldt-Ohmann H., Pyke A. T., Suhrbier A., Hall R. A. 2013; Neutralizing monoclonal antibodies to the E2 protein of chikungunya virus protects against disease in a mouse model. Clin Immunol 149:487–497 [View Article][PubMed]
    [Google Scholar]
  7. Grandadam M., Caro V., Plumet S., Thiberge J. M., Souarès Y., Failloux A. B., Tolou H. J., Budelot M., Cosserat D. other authors 2011; Chikungunya virus, southeastern France. Emerg Infect Dis 17:910–913 [View Article][PubMed]
    [Google Scholar]
  8. Hall R. A., Burgess G. W., Kay B. H. 1988; Type-specific monoclonal antibodies produced to proteins of Murray Valley encephalitis virus. Immunol Cell Biol 66:51–56 [View Article][PubMed]
    [Google Scholar]
  9. Hoarau J. J., Jaffar Bandjee M. C., Krejbich Trotot P., Das T., Li-Pat-Yuen G., Dassa B., Denizot M., Guichard E., Ribera A. other authors 2010; Persistent chronic inflammation and infection by chikungunya arthritogenic alphavirus in spite of a robust host immune response. J Immunol 184:5914–5927 [View Article][PubMed]
    [Google Scholar]
  10. Hong E. M., Perera R., Kuhn R. J. 2006; Alphavirus capsid protein helix I controls a checkpoint in nucleocapsid core assembly. J Virol 80:8848–8855 [View Article][PubMed]
    [Google Scholar]
  11. Horwood P., Bande G., Dagina R., Guillaumot L., Aaskov J., Pavlin B. 2013; The threat of chikungunya in Oceania. Western Pacific Surveill Response J 4:8–10 [View Article][PubMed]
    [Google Scholar]
  12. Jaffar-Bandjee M. C., Ramful D., Gauzere B. A., Hoarau J. J., Krejbich-Trotot P., Robin S., Ribera A., Selambarom J., Gasque P. 2010; Emergence and clinical insights into the pathology of chikungunya virus infection. Expert Rev Anti Infect Ther 8:987–996 [View Article][PubMed]
    [Google Scholar]
  13. Labadie K., Larcher T., Joubert C., Mannioui A., Delache B., Brochard P., Guigand L., Dubreil L., Lebon P. other authors 2010; Chikungunya disease in nonhuman primates involves long-term viral persistence in macrophages. J Clin Invest 120:894–906 [View Article][PubMed]
    [Google Scholar]
  14. Lam S. K., Chua K. B., Hooi P. S., Rahimah M. A., Kumari S., Tharmaratnam M., Chuah S. K., Smith D. W., Sampson I. A. 2001; Chikungunya infection – an emerging disease in Malaysia. Southeast Asian J Trop Med Public Health 32:447–451[PubMed]
    [Google Scholar]
  15. Lumsden W. H. 1955; An epidemic of virus disease in Southern Province, Tanganyika territory, in 1952–53. II. General description and epidemiology. Trans R Soc Trop Med Hyg 49:33–57 [View Article][PubMed]
    [Google Scholar]
  16. Mavalankar D., Shastri P., Bandyopadhyay T., Parmar J., Ramani K. V. 2008; Increased mortality rate associated with chikungunya epidemic, Ahmedabad, India. Emerg Infect Dis 14:412–415 [View Article][PubMed]
    [Google Scholar]
  17. Munasinghe D. R., Amarasekera P. J., Fernando C. F. 1966; An epidemic of dengue-like fever in Ceylon (chikungunya – a clinical and haematological study. Ceylon Med J 11:129–142[PubMed]
    [Google Scholar]
  18. Oliveira N. M., Broom A. K., Mackenzie J. S., Smith D. W., Lindsay M. D., Kay B. H., Hall R. A. 2006; Epitope-blocking enzyme-linked immunosorbent assay for detection of antibodies to Ross River virus in vertebrate sera. Clin Vaccine Immunol 13:814–817 [View Article][PubMed]
    [Google Scholar]
  19. Renault P., Solet J. L., Sissoko D., Balleydier E., Larrieu S., Filleul L., Lassalle C., Thiria J., Rachou E. other authors 2007; A major epidemic of chikungunya virus infection on Réunion Island, France, 2005–2006. Am J Trop Med Hyg 77:727–731[PubMed]
    [Google Scholar]
  20. Rezza G., Nicoletti L., Angelini R., Romi R., Finarelli A. C., Panning M., Cordioli P., Fortuna C., Boros S. other authors 2007; Infection with chikungunya virus in Italy: an outbreak in a temperate region. Lancet 370:1840–1846 [View Article][PubMed]
    [Google Scholar]
  21. Robinson M. C. 1955; An epidemic of virus disease in Southern Province, Tanganyika territory, in 1952–1953. I. Clinical features. Trans R Soc Trop Med Hyg 49:28–32 [View Article][PubMed]
    [Google Scholar]
  22. Rudd P. A., Wilson J., Gardner J., Larcher T., Babarit C., Le T. T., Anraku I., Kumagai Y., Loo Y. M. other authors 2012; Interferon response factors 3 and 7 protect against chikungunya virus hemorrhagic fever and shock. J Virol 86:9888–9898 [View Article][PubMed]
    [Google Scholar]
  23. Schuffenecker I., Iteman I., Michault A., Murri S., Frangeul L., Vaney M. C., Lavenir R., Pardigon N., Reynes J. M. other authors 2006; Genome microevolution of chikungunya viruses causing the Indian Ocean outbreak. PLoS Med 3:e263 [View Article][PubMed]
    [Google Scholar]
  24. Staples J. E., Breiman R. F., Powers A. M. 2009; Chikungunya fever: an epidemiological review of a re-emerging infectious disease. Clin Infect Dis 49:942–948 [View Article][PubMed]
    [Google Scholar]
  25. Strauss J. H., Strauss E. G. 1994; The alphaviruses: gene expression, replication, and evolution. Microbiol Rev 58:491–562[PubMed]
    [Google Scholar]
  26. Suhrbier A., Jaffar-Bandjee M. C., Gasque P. 2012; Arthritogenic alphaviruses – an overview. Nature Rev Rheumatol 8:420–429 [View Article][PubMed]
    [Google Scholar]
  27. Tandale B. V., Sathe P. S., Arankalle V. A., Wadia R. S., Kulkarni R., Shah S. V., Shah S. K., Sheth J. K., Sudeep A. B. other authors 2009; Systemic involvements and fatalities during chikungunya epidemic in India, 2006. J Clin Virol 46:145–149 [View Article][PubMed]
    [Google Scholar]
  28. Tesh R. B. 1982; Arthritides caused by mosquito-borne viruses. Annu Rev Med 33:31–40 [View Article][PubMed]
    [Google Scholar]
  29. Van Bortel W., Dorleans F., Rosine J., Blateau A., Rousset D., Matheus S., Leparc-Goffart I., Flusin O., Prat C. other authors 2014; Chikungunya outbreak in the Caribbean region, December 2013 to March 2014, and the significance for Europe. Euro Surveill 19:[PubMed]
    [Google Scholar]
  30. Viennet E., Knope K., Faddy H. M., Williams C. R., Harley D. 2013; Assessing the threat of chikungunya virus emergence in Australia. Commun Dis Intell Q Rep 37:E136–E143[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000002
Loading
/content/journal/jgv/10.1099/jgv.0.000002
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error