1887

Abstract

SUMMARY

Treatment of Semliki Forest virus with Nonidet-P40, sodium deoxycholate or Tween 80 + ether destroyed virus infectivity but preserved complement fixing, haem-agglutinating and neutralizing antibody blocking activities. All treatments split the virus into intact cores and fragments of envelope, but Nonidet gave the best separation of these components. Only the virus envelope had haemagglutinating and neutralizing antibody-blocking activities. Immunodiffusion tests showed that the envelope and core proteins were serologically distinct.

Further treatment of envelope fragments with dilute trypsin liberated at least three antigens, which could be separated by column chromatography. In immuno-diffusion tests, antigens 2 and 3 were serologically distinct, but both gave reactions of partial identity with antigen 1. All antigens fixed complement, but only antigen 1 was associated with haemagglutinating and neutralizing antibody-blocking activities. Sephadex gel filtration indicated that antigen 1 had a particle weight greater than 200,000 daltons, that antigen 3 had a molecular weight of about 9000, and that antigen 2 was intermediate in size.

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1970-12-01
2024-10-14
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References

  1. Andrews P. 1964; Estimation, of molecular weight of proteins by Sephadex gel-filtration. Biochemical Journal 91:222
    [Google Scholar]
  2. Appleyard G., Stanley J. L. 1970; Immunogenic subunits from Semliki Forest Virus. (In the Press)
    [Google Scholar]
  3. Clarke D. H., Casals J. 1958; Technique for haemagglutination and haemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561
    [Google Scholar]
  4. Crowle A. J. 1958; A simplified micro double-diffusion agar precipitin technique. Journal of Laboratory and Clinical Medicine 52:784
    [Google Scholar]
  5. Friedman R. M. 1968; Structural and nonstructural proteins of an arbovirus. Journal of Virology 2:1076
    [Google Scholar]
  6. Friedman R. M., Levy H. B., Carter W. B. 1966; Replication of Semliki Forest vims. Three forms of viral RNA produced during infection. Proceedings of the National Academy of Sciences of the United States of America 56:440
    [Google Scholar]
  7. Hay A. J., Skehel J. J., Burke D. C. 1968; Proteins synthesized in chick cells following infection with Semliki Forest virus. Journal of General Virology 3:175
    [Google Scholar]
  8. Horzinek M., Mussgay M. 1969; Studies on the nucleocapsid structure of a group A arbovirus. Journal of Virology 4:514
    [Google Scholar]
  9. Kääriäinen L., Simons K., Von Bonsdorff C.-H. 1969; Studies in subviral components of Semliki Forest virus. Annales Medicinae Experimentales et Biologiae Fenniae 47:235
    [Google Scholar]
  10. Lennette E. H. 1964 In Diagnostic Procedures for Viral and Rickettsial Diseases Ed. by Lennette E. H., Schmidt N. J. 3rd edition, p 57 New York: American Public Health Association, Inc;
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265
    [Google Scholar]
  12. Mussgay M., Horzinek M. 1966; Investigations on complement-fixing subunits of a group A arbovirus (Sindbis). Virology 29:199
    [Google Scholar]
  13. Mussgay M., Rott R. 1964; Studies on the structure of a haemaggiutinating component of a group A arbovirus (Sindbis). Virology 23:573
    [Google Scholar]
  14. Noll H., Aoyagi T., Orlando J. 1962; The structural relationship of sialidase to the influenza virus surface. Virology 18:154
    [Google Scholar]
  15. Robinson H. K., Wade H. E. 1968; The preparation of ribosomal ribonucleic acid from whole bacteria. Biochemical Journal 106:897
    [Google Scholar]
  16. Simons K., Kääriäinen L. 1970; Characterization of the Semliki Forest virus core and envelope proteins. Biochemical and Biophysical Research Communications 38:981
    [Google Scholar]
  17. Strauss J. H., Burge B. W., Darnell J. E. 1969; Sindbis virus infection of chick and hamster cells: synthesis of virus-specific proteins. Virology 37:367
    [Google Scholar]
  18. Strauss J. H., Burge B. W., Pfefferkorn E. R., Darnell J. E. 1968; Identification of the membrane protein and core protein of Sindbis virus. Proceedings of the National Academy of Sciences of the United States of America 50:533
    [Google Scholar]
  19. Zwartouw H. T., Algar D. J. 1968; Growth of high-titre Semliki Forest virus in concentrated suspensions of chick embryo cells. Journal of General Virology 2243
    [Google Scholar]
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