The incorporation of [3H]uridine into the four species of RNA specified by Sendai virus in monolayers of chick embryo fibroblasts was inhibited to an equal degree by addition of purified chick interferon to cultures before infection with virus. The inhibition of RNA synthesis was dose-dependent. Similarly, [3H]uridine incorporation into RNA of virus nucleocapsid and polyribosomes was completely inhibited by pretreatment with interferon. When interferon was added 2 hr after infection, there were only small effects on the synthesis of total virus-specific RNA and significantly greater reduction was observed in [3H]uridine incorporation into the RNA of nucleocapsid and polyribosomes. Although Sendai infection does not interfere with host-cell protein or RNA synthesis, interferon added 6 hr or later after infection did not affect any Sendai replicative functions.
BlairC. D.,
RobinsonW. S.1968; Replication of Sendai Virus I. Composition of viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology 35:537
FriedmanR. M.,
SonnabendJ. A.1965; Inhibition by interferon of production of double-stranded Semliki Forest virus ribonucleic acid. Nature, London 206:532
GordonI.,
ChenaultS. S.,
StevensonD.,
ActonJ. D.1966; Effect of interferon on polymerization of single-stranded and double-stranded Mengo virus ribonucleic acid. Journal of Bacteriology 91:1230
JoklikW. K.,
MeriganT. C.1966; Concerning the mechanism of action of interferon. Proceedings of the National Academy of Sciences of the United States of America 56:558
LampsonG. P.,
TytellA. A.,
NemesM. M.,
HillemanM. R.1963; Purification and characterization of chick embryo interferon. Proceedings of the Society for Experimental Biology and Medicine 112:468
LevineS.,
MageeW. E.,
HamiltonR. D.,
MillerO. V.1967; Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection. Virology 32:33
LockartR. Z.Jun.1964; The necessity for cellular RNA and protein synthesis for viral inhibition resulting from interferon. Biochemical and Biophysical Research Communications 15:513
MécsE.,
SonnabendJ. A.,
MartinE. M.,
FantesK. T.1967; The effect of interferon on the synthesis of RNA in chick cells infected with Semliki Forest virus. Journal of General Virology 1:25
MinerN.,
RayW. J.,
SimonE. H.1966; Effect of interferon on the production and action of viral RNA polymerase. Biochemical and Biophysical Research Communications 24:264
SonnabendJ. A.,
MartinE. M.,
MécsE.,
FantesK. H.1967; The effect of interferon on the synthesis and activity of an RNA polymerase isolated from chick cells infected with Semliki Forest virus. Journal of General Virology 1:41