1887

Abstract

Bovine viral diarrhoea virus (BVDV) envelope glycoprotein E interacts with highly sulphated heparin-like glycosaminoglycans (GAGs) located on the cell surface as an early step in virus infection of cells. Site-directed mutagenesis of recombinant E was undertaken and analysis of mutants by heparin-affinity chromatography and cell surface binding showed that a cluster of basic amino acids (KKLENKSK) near the C terminus of E was essential for binding. Mutants with amino acid substitutions of lysine residues 481 and 485 in E reduced the binding of E to immobilized heparin and cellular GAGs but retained ribonuclease activity. In contrast to normal E, E that was unable to bind to cells also failed to inhibit BVDV infection of cells when the cells were pre-incubated with E. It is proposed that the cluster of basic residues (KKLENKSK) localized at the C-terminal end of E constitutes a GAG-binding site.

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2002-09-01
2020-01-22
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