1887

Abstract

Hantavirus pulmonary syndrome (HPS) has been recognized increasingly as a significant public health problem in South America since Andes virus was first discovered in Argentina. Here, the isolation of Andes virus is reported from an infected rodent captured in Argentina in close vicinity to the place of the first HPS case, AH1. The complete nucleotide sequences of the virus M segment, partial L segment and the termini of the S, M and L segment genome RNAs were determined. The Andes virus M RNA segment is 3671 nt in length and is predicted to encode a glycoprotein precursor 1138 aa in length; it generally resembles the other HPS-associated hantaviruses in its organization. Relative to the G1 glycoprotein of other HPS-associated hantaviruses, an additional potential glycosylation site was found but this is located in the predicted cytoplasmic domain and is therefore unlikely to be glycosylated. In phylogenetic analyses, Andes virus, together with the more related hantaviruses, represented a monophyletic lineage. The S-terminal nucleotides were conserved relative to other New World hantaviruses. The M and L segment RNA termini had short deletions in the region believed to contain the sequence and structural features necessary for initiation of virus RNA replication and transcription. Clinical manifestations of Andes virus infections range from fulminant respiratory disease with high lethality to mild course without sequelae. Andes virus has also been associated with person-to-person transmission. Accumulation of Andes virus genetic data will be essential for understanding the factors that regulate virus replication and transmission and to determine the pathogenesis of HPS.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-9-2117
2002-09-01
2019-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/9/0832117a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-9-2117&mimeType=html&fmt=ahah

References

  1. Enria, D., Padula, P., Segura, E. L., Pini, N., Edelstein, A., Posse, C. R. & Weissenbacher, M. C. ( 1996; ). Hantavirus pulmonary syndrome in Argentina. Possibility of person to person transmission. Medicina 56, 709-711.
    [Google Scholar]
  2. Garcin, D., Lezzi, M., Dobbs, M., Elliott, R. M., Schmaljohn, C., Kang, C. Y. & Kolakofsky, D. ( 1995; ). The 5′ ends of Hantaan virus (Bunyaviridae) RNAs suggest a prime-and-realign mechanism for the initiation of RNA synthesis. Journal of Virology 69, 5754-5762.
    [Google Scholar]
  3. Johnson, A. M., Bowen, M. D., Ksiazek, T. G., Williams, R. J., Bryan, R. T., Mills, J. N., Peters, C. J. & Nichol, S. T. ( 1997; ). Laguna Negra virus associated with HPS in western Paraguay and Bolivia. Virology 238, 115-127.[CrossRef]
    [Google Scholar]
  4. Kukkonen, S. K., Vaheri, A. & Plyusnin, A. ( 1998; ). Completion of the Tula hantavirus genome sequence: properties of the L segment and heterogeneity found in the 3′ termini of S and L genome RNAs. Journal of General Virology 79, 2615-2622.
    [Google Scholar]
  5. Levis, S., Morzunov, S. P., Rowe, J. E., Enria, D., Pini, N., Calderon, G., Sabattini, M. & St Jeor, S. C. ( 1998; ). Genetic diversity and epidemiology of hantaviruses in Argentina. Journal of Infectious Diseases 177, 529-538.[CrossRef]
    [Google Scholar]
  6. López, N., Padula, P., Rossi, C., Lázaro, M. E. & Franze-Fernández, M. T. ( 1996; ). Genetic identification of a new hantavirus causing severe pulmonary syndrome in Argentina. Virology 220, 223-226.[CrossRef]
    [Google Scholar]
  7. López, N., Padula, P., Rossi, C., Miguel, S., Edelstein, A., Ramı́rez, E. & Franze-Fernández, M. T. ( 1997; ). Genetic characterization and phylogeny of Andes virus and variants from Argentina and Chile. Virus Research 50, 77-84.[CrossRef]
    [Google Scholar]
  8. Meyer, B. J. & Southern, P. J. ( 1994; ). Sequence heterogeneity in the termini of lymphocytic choriomeningitis virus genomic and antigenomic RNAs. Journal of Virology 68, 7659-7664.
    [Google Scholar]
  9. Meyer, B. J. & Southern, P. J. ( 1997; ). A novel type of defective viral genome suggests a unique strategy to establish and maintain persistent lymphocytic choriomeningitis virus infections. Journal of Virology 71, 6757-6764.
    [Google Scholar]
  10. Meyer, B. J. & Schmaljohn, C. S. ( 2000; ). Persistent hantavirus infections: characteristics and mechanisms. Trends in Microbiology 8, 61-67.[CrossRef]
    [Google Scholar]
  11. Padula, P. J., Edelstein, A., Miguel, S. D. L., López, N. M., Rossi, C. M. & Rabinovich, R. D. ( 1998; ). Hantavirus pulmonary syndrome outbreak in Argentina: molecular evidence for person-to-person transmission of Andes virus. Virology 241, 323-330.[CrossRef]
    [Google Scholar]
  12. Padula, P. J., Colavecchia, S. B., Martinez, V. P., Gonzalez Della Valle, M. O., Edelstein, A., Miguel, S. D., Russi, J., Riquelme, J. M., Colucci, N., Almiron, M. & Rabinovich, R. D. ( 2000; ). Genetic diversity, distribution, and serological features of hantavirus infection in five countries in South America. Journal of Clinical Microbiology 38, 3029-3035.
    [Google Scholar]
  13. Parrington, M. A., Lee, P. W. & Kang, C. Y. ( 1991; ). Molecular characterization of the Prospect Hill virus M RNA segment: comparison with the M RNA segments of other hantaviruses. Journal of General Virology 72, 1845-1854.[CrossRef]
    [Google Scholar]
  14. Schmaljohn, C. S. ( 1996; ). Molecular biology of hantaviruses. In The Bunyaviridae , pp. 63-90. Edited by R. M. Elliott. New York: Plenum.
  15. Toro, J., Vega, J. D., Khan, A. S., Mills, J. N., Padula, P. J., Terry, W., Yadón, Z., Valderrama, R., Ellis, B. A., Pavletic, C., Cerda, R., Zaki, S., Shieh, W.-J., Meyer, R., Tapia, M., Mansilla, C., Baro, M., Vergara, J. A., Concha, M., Calderón, G., Enria, D., Peters, C. J. & Ksiazek, T. G. ( 1998; ). An outbreak of hantavirus pulmonary syndrome, Chile, 1997. Emerging Infectious Diseases 4, 687-694.[CrossRef]
    [Google Scholar]
  16. Wells, R. M., Sosa Estani, S. S., Yadón, Z. E., Enria, D., Padula, P., Pini, N., Mills, J. N., Peters, C. J. & Segura, E. L. ( 1997; ). An unusual hantavirus outbreak in southern Argentina: person-to-person transmission? Hantavirus Pulmonary Syndrome Study Group for Patagonia. Emerging Infectious Diseases 3, 171-174.[CrossRef]
    [Google Scholar]
  17. Zuker, M. ( 1989; ). On finding all suboptimal foldings of an RNA molecule. Science 224, 48-52.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-9-2117
Loading
/content/journal/jgv/10.1099/0022-1317-83-9-2117
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error