RT Journal Article SR Electronic(1) A1 Léonard, Simon A1 Chisholm, Joan A1 Laliberté, Jean-François A1 Sanfaçon, HélèneYR 2002 T1 Interaction in vitro between the proteinase of Tomato ringspot virus (genus Nepovirus) and the eukaryotic translation initiation factor iso4E from Arabidopsis thaliana JF Journal of General Virology, VO 83 IS 8 SP 2085 OP 2089 DO https://doi.org/10.1099/0022-1317-83-8-2085 PB Microbiology Society, SN 1465-2099, AB Eukaryotic initiation factor eIF(iso)4E binds to the cap structure of mRNAs leading to assembly of the translation complex. This factor also interacts with the potyvirus VPg and this interaction has been correlated with virus infectivity. In this study, we show an interaction between eIF(iso)4E and the proteinase (Pro) of a nepovirus (Tomato ringspot virus; ToRSV) in vitro. The ToRSV VPg did not interact with eIF(iso)4E although its presence on the VPg-Pro precursor increased the binding affinity of Pro for the initiation factor. A major determinant of the interaction was mapped to the first 93 residues of Pro. Formation of the complex was inhibited by addition of m7GTP (a cap analogue), suggesting that Pro-containing molecules compete with cellular mRNAs for eIF(iso)4E binding. The possible implications of this interaction for translation and/or replication of the virus genome are discussed., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-83-8-2085