1887

Abstract

Polydnaviruses are unique viruses: they are essential for successful parasitism by tens of thousands of species of parasitoid wasps. These viruses are obligatorily associated with the wasps and are injected into the host during oviposition. Molecular analyses have shown that each virus sequence in the segmented polydnavirus genome is present in the wasp DNA in two forms: a circular form found in the virus particles and an integrated form found in the wasp chromosomes. Recent studies performed on polydnaviruses from braconid wasps suggested that the circular forms were excised from the chromosome. The different forms of the EP1 circle of polydnavirus during the pupal–adult development of the parasitoid wasp were analysed. Unexpectedly, an off-size fragment formerly used to diagnose the integration of the EP1 sequence into wasp genomic DNA was found to be amplified in female wasps undergoing virus replication. The EP1 sequence is amplified within a larger molecule comprising at least two virus segments. The amplified molecule is different from the EP1 chromosomally integrated form and is not encapsidated into virus particles. These findings shed light on a new step towards EP1 circle production: the amplification of virus sequences preceding individual circle excision.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-8-2035
2002-08-01
2020-09-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/8/0832035a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-8-2035&mimeType=html&fmt=ahah

References

  1. Albrecht U., Wyler T., Pfister-Wilhelm R., Gruber A., Stettler P., Heiniger P., Kurt E., Schümperli D., Lanzrein B.. 1994; Polydnavirus of the parasitic wasp Chelonus inanitus (Braconidae): characterization, genome organization and time point of replication. Journal of General Virology75:3353–3363
    [Google Scholar]
  2. Beckage N. E.. 1998; Modulation of immune responses to parasitoids by polydnaviruses. Parasitology116:57–64
    [Google Scholar]
  3. Beckage N. E., Tan F. F., Schleifer K. W., Lane R. D., Cherubin L. L.. 1994; Characterisation and biological effects of Cotesia congregata polydnavirus on host larvae of the tobacco hornworm, Manduca sexta . Archives of Insect Biochemistry and Physiology26:165–195
    [Google Scholar]
  4. Belle E., Beckage N. E., Rousselet J., Poirié M., Lemeunier F., Drezen J.-M.. 2002; Visualization of polydnavirus sequences in a parasitoid wasp chromosome. Journal of Virology76:5793–5796
    [Google Scholar]
  5. Bostock C. J.. 1986; Mechanisms of DNA sequence amplification and their evolutionary consequences. Philosophical Transactions of the Royal Society of London B Biological Sciences312:261–273
    [Google Scholar]
  6. Cui L., Webb B. A.. 1997; Homologous sequences in the Campoletis sonorensis polydnavirus genome are implicated in replication and nesting of the W segment family. Journal of Virology71:8504–8513
    [Google Scholar]
  7. De Buron I., Beckage N. E.. 1992; Characterization of a polydnavirus (PDV) and virus-like filamentous particle (VLFP) in the braconid wasp Cotesia congregata (Hymenoptera: Braconidae). Journal of Invertebrate Pathology59:315–327
    [Google Scholar]
  8. Deng L., Stoltz D. B., Webb B. A.. 2000; A gene encoding a polydnavirus structural polypeptide is not encapsidated. Virology269:440–450
    [Google Scholar]
  9. Dowton M., Austin A. D.. 1998; Phylogenetic relationships among the microgastroid wasps (Hymenoptera: braconidae): combined analysis of 16S and 28S rDNA genes and morphological data. Molecular Phylogenetics and Evolution10:354–366
    [Google Scholar]
  10. Drezen J.-M., Huguet E., Dupuy C., Poirié M.. 2000; Polydnaviruses: how viruses evolve after domestication. In Research Advances in Virology pp45–77 Edited by Mohan R.. Puthen Chalai: Global Research Network;
    [Google Scholar]
  11. Dushay M. S., Beckage N. E.. 1993; Dose-dependent separation of Cotesia congregata associated polydnavirus effects on Manduca sexta larval development and immunity. Journal of Insect Physiology39:1029–1040
    [Google Scholar]
  12. Fleming J. A. G. W., Summers M. D.. 1991; Polydnavirus DNA is integrated in the DNA of its parasitoid wasp host. Proceedings of the National Academy of Sciences, USA88:9770–9774
    [Google Scholar]
  13. Gruber A., Stettler P., Heiniger P., Schumperli D., Lanzrein B.. 1996; Polydnavirus DNA of the braconid wasp Chelonus inanitus is integrated in the wasp′s genome and excised only in later pupal and adult stages of the female. Journal of General Virology77:2873–2879
    [Google Scholar]
  14. Hamm J., Styer E. L., Lewis W. J.. 1990; Comparative virogenesis of filamentous virus and polydnavirus in the female reproductive tract of Cotesia marginiventris (Hymenoptera: Braconidae). Journal of Invertebrate Pathology55:357–374
    [Google Scholar]
  15. Harwood S. H., Grosovsky A. J., Cowles E. A., Davis J. W., Beckage N. E.. 1994; An abundantly expressed hemolymph glycoprotein isolated from newly parasitized Manduca sexta larvae is a polydnavirus gene product. Virology205:381–392
    [Google Scholar]
  16. Osheim Y. N., Miller O. L. Jr, Beyer A. L.. 1988; Visualization of Drosophila melanogaster chorion genes undergoing amplification. Molecular and Cellular Biology8:2811–2821
    [Google Scholar]
  17. Savary S., Beckage N. E., Tan F., Periquet G., Drezen J. M.. 1997; Excision of the polydnavirus chromosomal integrated EP1 sequence of the parasitoid wasp Cotesia congregata (Braconidae, Microgastrinae) at potential recombinase binding sites. Journal of General Virology78:3125–3134
    [Google Scholar]
  18. Savary S., Drezen J. M., Tan F., Beckage N. E., Periquet G.. 1999; The excision of polydnavirus sequences from the genome of the wasp Cotesia congregata (Braconidae, Microgastrinae) is developmentally regulated but not strictly restricted to the ovaries in the adult. Insect Molecular Biology8:319–327
    [Google Scholar]
  19. Shelby K. S., Webb B. A.. 1999; Polydnavirus-mediated suppression of insect immunity. Journal of Insect Physiology45:507–514
    [Google Scholar]
  20. Soller M., Lanzrein B.. 1996; Polydnavirus and venom of the egg-larval parasitoid Chelonus inanitus (Braconidae) induce developmental arrest in the prepupa of its host Spodoptera littoralis (Noctuidae). Journal of Insect Physiology42:471–481
    [Google Scholar]
  21. Spradling A. C.. 1981; The organization and amplification of two chromosomal domains containing Drosophila chorion genes. Cell27:193–201
    [Google Scholar]
  22. Stoltz D. B.. 1993; The polydnavirus life cycle. In Parasites and Pathogens of Insects . pp80–101 Edited by Beckage N. E., Thomson S. N., Federici B. A.. San Diego: Academic Press;
  23. Summers M. D., Dib-Hajj S. D.. 1995; Polydnavirus-facilitated endoparasite protection against host immune defences. Proceedings of the National Academy of Sciences, USA92:29–36
    [Google Scholar]
  24. Theilmann D. A., Summers M. D.. 1986; Molecular analysis of Campoletis sonorensis virus DNA in the lepidopteran host Heliothis virescens . Journal of General Virology67:1961–1969
    [Google Scholar]
  25. Volkoff A. N., Ravallec M., Bossy J., Cerutti P., Rocher J., Cerutti M., Devauchelle G.. 1995; The replication of Hyposoter didymator polydnavirus: cytopathology of the calyx cells in the parasitoid. Biological Cell83:1–13
    [Google Scholar]
  26. Webb B. A.. 1998; Polydnavirus biology, genome structure, and evolution. In The Insect Viruses pp105–139 Edited by Miller L. K., Ball L. A.. New York: Plenum;
    [Google Scholar]
  27. Webb B. A., Summers M. D.. 1992; Stimulation of polydnavirus replication by 20-hydroxyecdysone. Experientia (Basel)48:1018–1022
    [Google Scholar]
  28. Whitfield J. B.. 1997; Molecular and morphological data suggest a single origin of the polydnaviruses among braconid wasps. Naturwissenschaften84:502–507
    [Google Scholar]
  29. Whitfield J. B.. 2000; Phylogeny of microgastroid braconid wasps, and what it tells us about polydnavirus evolution. In The Hymenoptera: Evolution Biodiversity and Biological Control pp97–105 Edited by Austin A. D., Dowton M.. Melbourne: CSIRO;
    [Google Scholar]
  30. Xu D., Stoltz D. B.. 1991; Evidence for a chromosomal location of polydnavirus DNA in the ichneumonid parasitoid Hyposoter fugitivus . Journal of Virology65:6693–6704
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-8-2035
Loading
/content/journal/jgv/10.1099/0022-1317-83-8-2035
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error