%0 Journal Article %A Schumacher, Daniel %A Tischer, B. Karsten %A Teifke, Jens-Peter %A Wink, Kerstin %A Osterrieder, Nikolaus %T Generation of a permanent cell line that supports efficient growth of Marek′s disease virus (MDV) by constitutive expression of MDV glycoprotein E %D 2002 %J Journal of General Virology, %V 83 %N 8 %P 1987-1992 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-83-8-1987 %I Microbiology Society, %X A recombinant cell line (SOgE) was established, which was derived from the permanent quail muscle cell line QM7 and constitutively expressed the glycoprotein E (gE) gene of Marek′s disease virus serotype 1 (MDV-1). The SOgE cell line supported growth of virulent (RB-1B) and vaccine (CVI988, 584Ap80C) MDV-1 strains at a level comparable with that of primary chicken embryo cells (CEC). The SOgE cell line was used to produce a vaccine against Marek′s disease. Chickens were immunized at 1 day old with 103 p.f.u. CVI988 produced on either CEC or SOgE cells. Challenge infection was performed at day 12 with hypervirulent Italian MDV-1 strain EU1. Whereas 7/7 or 6/6 animals, respectively, immunized with SOgE or QM7 cells alone developed Marek′s disease, only 1/8 animals from both CVI988-immunized groups exhibited signs of disease, suggesting that SOgE cells are a valuable permanent cell culture system for MDV-1 vaccine production. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-83-8-1987