1887

Abstract

Full-length and partial genome sequences of four members of the genus , family (Golden shiner reovirus, Grass carp reovirus, Striped bass reovirus and golden ide reovirus) were characterized. Based on sequence comparison, the unclassified Grass carp reovirus was shown to be a member of the species . The status of golden ide reovirus, another unclassified aquareovirus, was also examined. Sequence analysis showed that it did not belong to the species or , but assessment of its relationship to the species , , and was hampered by the absence of genetic data from these species. In agreement with previous reports of ultrastructural resemblance between aquareoviruses and orthoreoviruses, genetic analysis revealed homology in the genes of the two groups. This homology concerned eight of the 11 segments of the aquareovirus genome (amino acid identity 17–42%), and similar genetic organization was observed in two other segments. The conserved terminal sequences in the genomes of members of the two groups were also similar. These data are undoubtedly an indication of the common evolutionary origin of these viruses. This clear genetic relatedness between members of distinct genera is unique within the family . Such a genetic relationship is usually observed between members of a single genus. However, the current taxonomic classification of aquareoviruses and orthoreoviruses in two different genera is supported by a number of characteristics, including their distinct G+C contents, unequal numbers of genome segments, absence of an antigenic relationship, different cytopathic effects and specific econiches.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-8-1941
2002-08-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/8/0831941a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-8-1941&mimeType=html&fmt=ahah

References

  1. Anzola, J. V., Xu, Z. K., Asamizu, T. & Nuss, D. L. ( 1987; ). Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Proceedings of the National Academy of Sciences, USA 84, 8301-8305.[CrossRef]
    [Google Scholar]
  2. Attoui, H., Billoir, F., Cantaloube, J.-F., Biagini, P., de Micco, P. & de Lamballerie, X. ( 2000; ). Strategies for the sequence determination of viral dsRNA genomes. Journal of Virological Methods 89, 147-158.[CrossRef]
    [Google Scholar]
  3. Attoui, H., Mohd Jaafar, F., Biagini, P., Cantaloube, J.-F., de Micco, P., Murphy, F. A. & de Lamballerie, X. ( 2002; ). Genus Coltivirus (family Reoviridae): genomic and morphologic characterization of Old World and New World viruses. Archives of Virology 147, 533-561.[CrossRef]
    [Google Scholar]
  4. Chen, B. S. & Jiang, Y. ( 1984; ). Morphological and physicochemical characterization of the hemorrhagic virus of grass carp. Kexue Tongbao 29, 832–835 (in Chinese).
    [Google Scholar]
  5. Fang, Q., Ke, L. H. & Cai, Y. Q. ( 1989; ). Growth characterization and high titre culture of GCHV. Virologica Sinica 3, 315-319.
    [Google Scholar]
  6. Fang, Q., Attoui, H., Cantaloube, J. F., Biagini, P., Zhu, Z., de Micco, P. & de Lamballerie, X. ( 2000; ). Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (genus Aquareovirus, family Reoviridae). Biochemical and Biophysical Research Communications 274, 762-766.[CrossRef]
    [Google Scholar]
  7. Francki, R. I. B., Fauquet, C. M., Knudson, D. L. & Brown, F. (1991). Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Wien: Springer-Verlag.
  8. Ke, L. H., Fang, Q. & Cai, Y. Q. ( 1990; ). Characteristics of a novel isolate of grass carp hemorrhage virus [J]. Acta Hydrobiologica Sinica 14, 153-159.
    [Google Scholar]
  9. Kumar, S., Tamura, K. & Nei, M. (1993). Molecular Evolutionary Genetics Analysis, version 1.01. Pennsylvania State University.
  10. Kyte, J. & Doolittle, R. F. ( 1982; ). A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157, 105-132.[CrossRef]
    [Google Scholar]
  11. Mertens, P. P. C., Arella, M., Attoui, H., Belloncik, S., Bergoin, M., Boccardo, G., Booth, T. F., Chiu, W., Diprose, J. M., Duncan, R. and 34 others (2000). Family Reoviridae. In Virus Taxonomy. Seventh Report of the International Committee for the Taxonomy of Viruses, pp. 395–480. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego: Academic Press.
  12. Montanie, H., Bossy, J.-P. & Bonami, J.-R. ( 1993; ). Morphological and genomic characterization of two reoviruses (P and W2) pathogenic for marine crustaceans; do they constitute a novel genus of the Reoviridae family? Journal of General Virology 74, 1555-1561.[CrossRef]
    [Google Scholar]
  13. Neukirch, M., Haas, L., Lehmann, H. & von Messeling, V. ( 1999; ). Preliminary characterization of a reovirus isolated from golden ide Leuciscus idus melanotus. Diseases of Aquatic Organisms 35, 159-164.[CrossRef]
    [Google Scholar]
  14. Page, R. D. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357-358.
    [Google Scholar]
  15. Plumb, J. A., Bowser, P. R., Grizzle, J. M. & Mitchell, A. J. ( 1979; ). Fish viruses: a double stranded RNA icosahedral virus from a North American cyprinid. Journal of the Fisheries Research Board of Canada 36, 1390-1394.[CrossRef]
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406-425.
    [Google Scholar]
  17. Shaw, A. L., Samal, S. K., Subramanian, K. & Prasad, B. V. V. ( 1996; ). The structure of aquareovirus shows how the different geometries of the two layers of the capsid are reconciled to provide symmetrical interactions and stabilization. Structure 4, 957-967.[CrossRef]
    [Google Scholar]
  18. Subramanian, K., McPhillips, T. H. & Samal, S. K. ( 1994; ). Characterization of the polypeptides and determination of the genome coding assignments of an aquareovirus. Virology 205, 75-81.[CrossRef]
    [Google Scholar]
  19. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673-4680.[CrossRef]
    [Google Scholar]
  20. Winton, J. R., Lannan, C. N., Fryer, J. L., Hedrick, R. P., Meyers, T. R., Plumb, J. A. & Yamamoto, T. ( 1987; ). Morphological and biochemical properties of four members of a novel group of reoviruses isolated from aquatic animals. Journal of General Virology 68, 353-364.[CrossRef]
    [Google Scholar]
  21. Xu, Z. K., Anzola, J. V., Nalin, C. M. & Nuss, D. L. ( 1989; ). The 3′-terminal sequence of a wound tumor virus transcript can influence conformational and functional properties associated with the 5′-terminus. Virology 170, 511-522.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-8-1941
Loading
/content/journal/jgv/10.1099/0022-1317-83-8-1941
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error